Genetic risk stratification and risk factors of early menopause in women: a multi-center study utilizing polygenic risk scores.

Objective: This study aims to evaluate the utility of polygenic risk scores (PRS) in women with early menopause (EM) and to investigate the clinical characteristics and risk factors associated with EM based on genetic risk.

Study Design: Genotyping data and clinical data from women with EM and women with normal age of menopause retrieved from UK Biobank were used for early menopause risk prediction model establishment. Subsequently, 99 women diagnosed with EM and 1027 control women underwent PGT-M were recruited for model validation from across eight hospitals in China.

Manipulating Charge Distribution of Graphitic Carbon Nitride for Boosting NIR-II Light-Activated Reactive Oxygen Species Generation.

Structure engineering is of great importance to enhance the carrier separation efficiency of multiphoton absorption (MPA) materials for near-infrared (NIR) light-driven reactive oxygen species (ROS) generation. In this study, the MPA-responsive potassium/cyano group-functionalized graphitic carbon nitride was investigated, demonstrating charge redistribution and improved carrier separation efficiency by density functional theory calculations and experimental results. With various types of boosted ROS generation under UV-vis or NIR-II light irradiation, the potassium/cyano group-functionalized graphitic carbon nitride could achieve efficient multiphoton photodynamic therapy after reducing the particle size.

Personalized embryo transfer based on RNA sequencing endometrial receptivity test in repeated implantation failure patients: artificial cycle versus natural cycle.

Introduction: Embryo implantation provides an efficient way for patients with repeated implantation failure (RIF) to achieve pregnancy. The aim of this study is to compare the implantation outcomes of RIF patients in artificial cycle to those in natural cycle, both were treated with RNA sequencing endometrial receptivity test (rsERT) based personalized embryo implantation.

Methods: The endometrial receptivity (ER) analysis was performed using rsERT followed by personalized embryo transfer at optimal window of implantation (WOI).

Anterior uterine incarceration complicated by placenta previa and placenta accreta spectrum disorder: a case report.

Background: Uterine incarceration is a rare obstetric complication that is always associated with retroversion and prone to misdiagnosis. Pelvic examination and imaging methods including ultrasound and magnetic resonance imaging (MRI) are used as the primary diagnostic tool. We present an asymptomatic anterior uterine incarceration complicated by placenta previa and placenta accreta spectrum (PAS) disorder, which could be diagnosed during the pregnancy periods, but was first diagnosed during the cesarean section (CS) and got the surgeons into trouble.

Efficacy of low-dose hCG on FET cycle in patients with recurrent implantation failure.

Objective: To study patients' new treatment methods and mechanisms of repeated implantation failure.

Design: A retrospective study.

Setting: fertilization (IVF) unit in a Three-A hospital.

Long-term vaccine efficacy of a 2-dose varicella vaccine in China from 2011 to 2021: A retrospective observational study.

Objective: A 2-dose varicella vaccine immunization strategy has been implemented in many cities in China, but there is few evidence on a long-term evaluation of the efficacy of the 2-dose varicella vaccine from China. This study aims to assess the long-term vaccine efficacy of the two doses varicella vaccine and analysis of its influencing factors.

Methods: A retrospective study was carried out in 837,144 children born between 2011 and 2017 in Ningbo, Easten China.

The mining and construction of a knowledge base for gene-disease association in mitochondrial diseases.

Mitochondrial diseases are a group of heterogeneous genetic metabolic diseases caused by mitochondrial DNA (mtDNA) or nuclear DNA (nDNA) gene mutations. Mining the gene-disease association of mitochondrial diseases is helpful for understanding the pathogenesis of mitochondrial diseases, for carrying out early clinical diagnosis for related diseases, and for formulating better treatment strategies for mitochondrial diseases. This project researched the relationship between genes and mitochondrial diseases, combined the Malacards, Genecards, and MITOMAP disease databases to mine the knowledge on mitochondrial diseases and genes, used database integration and the sequencing method of the phenolyzer tool to integrate disease-related genes from different databases, and sorted the disease-related candidate genes.

Electrofusion Stimulation Is an Independent Factor of Chromosome Abnormality in Mice Oocytes Reconstructed Spindle Transfer.

Oocytes reconstructed by spindle transfer (ST) are prone to chromosome abnormality, which is speculated to be caused by mechanical interference or premature activation, the mechanism is controversial. In this study, C57BL/6N oocytes were used as the model, and electrofusion ST was performed under normal conditions, Ca free, and at room temperature, respectively. The effect of enucleation and electrofusion stimulation on MPF activity, spindle morphology, γ-tubulin localization and chromosome arrangement was compared.

Varicella breakthrough infection and effectiveness of 2-dose varicella vaccine in China.

Background: 2-dose varicella vaccination has been available in Ningbo city, China since 2014 in the private sector. This study aimed to evaluate the breakthrough varicella infection rate, and to examine overall and incremental effectiveness of 2-dose varicella vaccination among Chinese children.

Methods: A retrospective investigation was done among native children born from 2008 to 2013 in active surveillance area in Ningbo, China.

Comprehensive chromosomal and mitochondrial copy number profiling in human IVF embryos.

Single cell whole genome sequencing helps to decipher the genome heterogeneity within a cell population and facilitates the analysis of trace amounts of genetic material, such as is found in human embryos. The mitochondrial genome, although an important part of the genetic composition of eukaryotic cells, is often neglected in single cell genome analysis. A recently developed single cell whole genome amplification method was used, known as multiple annealing and looping based amplification cycles (MALBAC-NGS), for simultaneous analysis of chromosomal and mitochondrial genomes at the single cell level.

Individualized Hormone Adjustment in the Treatment of Recurrent Spontaneous Abortions.

Our goal was to develop a safe, efficient, and practical clinical plan for successful pregnancies for patients with recurrent spontaneous miscarriages by adjustment of their hormone levels after ovulation. We treated 61 patients with recurrent miscarriages and 110 patients with two miscarriages. All patients had miscarriages before or during the 12th week of pregnancy, and unsuccessfully underwent progesterone therapy.