Evaluation of Cyprinid Herpesvirus 2 Latency and Reactivation in .

Cyprinid herpesvirus 2 (CyHV-2, species ) causes severe mortality in ornamental goldfish, crucian carp (), and gibel carp (). It has been shown that the genomic DNA of CyHV-2 could be detected in subclinical fish, which implied that CyHV-2 could establish persistent infection. In this study, the latency of CyHV-2 was investigated in the survival fish after primary infection.

Effect of oxidized LDL on the expression of connexins in cultured human umbilical-vein endothelial cells.

The role of connexins in atherogenesis has attracted increasing attention in recent years; however, whether oxidized LDL (low density lipoprotein) is one of the pro-atherosclerotic factors that can influence the expression of endothelial connexins remains unclear. To investigate the effect of oxidized LDL on the gene expression of the gap junction proteins connexin37, connexin40 and connexin43 in cultured HUVEC (human umbilical-vein endothelial cells) in vitro, HUVEC were cultured and divided into groups before being stimulated with various concentrations of oxidized LDL for different times. mRNA expression of connexin37, connexin40 and connexin43 in each group were determined semi-quantitatively by RT-PCR (reverse transcription-PCR), while protein expression of the three connexins was investigated by immunocytochemical staining.