Complement components of nerve regeneration conditioned fluid influence the microenvironment of nerve regeneration.

Nerve regeneration conditioned fluid is secreted by nerve stumps inside a nerve regeneration chamber. A better understanding of the proteinogram of nerve regeneration conditioned fluid can provide evidence for studying the role of the microenvironment in peripheral nerve regeneration. In this study, we used cylindrical silicone tubes as the nerve regeneration chamber model for the repair of injured rat sciatic nerve.

Construction and identification of multiple genes Co silence of plasmid shRNA.

Objective To construct and identify the eukaryotic vector expressing shRNA (Plasmid-1), which expressed the VEGF, C-myc, Survivin and hTERT gene at the same time. To detect its interference effects on the nasopharyngeal carcinoma cell line (CNE-2Z) compared with single gene plasmid VEFG (Plasmid-2). Methods According to the sequence of VEGF, C-myc, Survivin and hTERT gene, we designed 2 oligonucleotide sequences and synthesized a complementary DNA chain, then inserted it into the eukaryotic vector expressing pGenesil 1.

Idiopathic recurrent facial palsy: Facial nerve decompression via middle cranial fossa approach.

Objective: To introduce our experience of preventing further episodes of idiopathic recurrent facial palsy by facial nerve decompression via middle cranial fossa approach.

Methods: Twelve cases (surgery group) who had idiopathic recurrent facial palsy underwent facial nerve decompression via middle cranial fossa approach, and 6 cases (control group) who declined surgery accepted conservative treatment. Further episodes of facial palsy and final outcomes of facial nerve were recorded.

[Analyses prognostic factors relevant to sudden sensorineural hearing loss].

Objective: To investigate the prognostic factors relevant to sudden sensorineural hearing loss.

Methods: The internationally accepted standardized clinical research methods, unified design, and unified program were adopted to conduct the prospective clinical multi-center study. The sudden deafness patients between 18 to 65 years old, with the course of this disorder less than two weeks, and without any medical treatments were collected, and then, divided into four types according to the hearing curve: type A, acute sensorineural hearing loss in low tone frequencies; type B, acute sensorineural hearing loss in high tone frequencies; type C, acute sensorineural hearing loss in all frequencies; and type D, total deafness.

Effects of different combinations of growth factors on the differentiation of neural stem cells into hair-like cells.

The differentiation of neural stem cells (NSCs) is influenced by a variety of factors. Therefore, it is important to explore the best external conditions that will induce NSCs to differentiate into hair cells. In this study, we investigated the best in vitro conditions for differentiation of NSCs derived from the hippocampus of newborn guinea pigs into hair-like cells.

Lipopolysaccharide-induced middle ear inflammation disrupts the cochlear intra-strial fluid-blood barrier through down-regulation of tight junction proteins.

Middle ear infection (or inflammation) is the most common pathological condition that causes fluid to accumulate in the middle ear, disrupting cochlear homeostasis. Lipopolysaccharide, a product of bacteriolysis, activates macrophages and causes release of inflammatory cytokines. Many studies have shown that lipopolysaccharides cause functional and structural changes in the inner ear similar to that of inflammation.

Structural changes in thestrial blood-labyrinth barrier of aged C57BL/6 mice.

Tight control over cochlear blood flow (CoBF) and the blood-labyrinth barrier (BLB) in the striavascularis is critical for maintaining the ionic, fluid and energy balance necessary for hearing function. Inefficient CoBF and disruption of BLB integrity have long been considered major etiologic factors in a variety of hearing disorders. In this study, we investigate structural changes in the BLB of the striavascularis in age-graded C57BL/6 mice (1 to 21 months) with a focus on changes in two blood barrier accessory cells, namely pericytes (PCs) and perivascular-resident macrophage-like melanocytes (PVM/Ms).

Ethical issues in Chinese aesthetic surgery.

Guided by the medical ethics principles of "four principles plus scope," Chinese aesthetic medical practitioners have proposed some extremely valuable ethical principles combined with the construction of aesthetic medicine and the requirements of clinical practice such as the principle of general nonmaleficence, the principle of local minimal invasiveness, the principle of informed consent, and the principle of respect and confidentiality. Chinese aesthetic surgical ethics provide valuable guidance for the practice of aesthetic medicine. Adherence to the ethics of Chinese aesthetic surgery provides an essential guide for the practice of aesthetic medicine in China.

[Clinical analysis of 26 patients with relapsing polychondritis].

Objective: To discuss the clinical characteristics and early diagnosis and treatment of relapsing polychondritis(RP).

Method: Twenty-six RP patients received the therapies of antibiotics, glucocorticosteroid, immunosuppressive agent,tracheotomy, tracheal sent implantation or non-invasive ventilation.

Result: Twenty-six cases (88.

Internal Mammary Artery Perforator-Supercharged Prefabricated Cervicothoracic Flap for Face and Neck Reconstruction.

Background: Prefabricated flap is an important technique to reconstruct massive face and neck skin defects. But its vascularization remains unpredictable and often leads to abnormal blood supply of the harvested flap, even necrosis. Flap supercharging and turbo supercharging techniques are effectively used to improve flap blood supply.

Suppression of T-type Ca2+ channels inhibited human laryngeal squamous cell carcinoma cell proliferation running title: roles of T-type Ca2+ channels in LSCC cell proliferation.

Background: Despite the tight correlation between T-type Ca2+ channels and a great variety of tumors, the roles of alpha1G subunit of T-type Ca2+ channels in laryngeal squamous cell carcinoma (LSCC) have not yet been investigated.

Methods: In the present study, we examined the expression of alpha1G subunit of T-type Ca2+ channel in human LSCC tissues and cell lines. One human laryngeal squamous cell carcinoma cell line, Hep-2, was also examined for T-type channels using voltage-clamp recordings.

[Effect of H-uvulopalatopharyngoplasty on endothelium dependent flow-mediated dilation in patients with moderate to severe obstructive sleep apnea syndrome].

Objective: This study evaluates the effects of H-uvulopalatopharyngoplasty (H-UPPP) on endothelial function by endothelium dependent flow-mediated dilation(FMD) in patients with moderate to severe obstructive sleep apnea syndrome (OSAHS).

Method: Forty-five healthy subjects and 49 patients with moderately to severe OSAHS who desired H-UPPP were prospectively enrolled. FMD was measured by high-resolution B-mode ultrasonography.

[The observation on curative effect of 90 cases of sudden hearing loss].

Objective: To evaluate the efficiency between two treatments of sudden hearing loss.

Method: All patients were divided into two groups randomly, basic drug group was treated with ganglioside and vinpocetine injection, combined therapy group was treated with intratympanic dexamethasone and what was used in basic drug group.

Result: The effective rate of combined therapy group (73.

Effect of Han-uvulopalatopharyngoplasty on flow-mediated dilatation in patients with moderate or severe obstructive sleep apnea syndrome.

Conclusion: Flow-mediated dilatation (FMD) is decreased in patients with moderate or severe obstructive sleep apnea syndrome (OSAS), and Han-uvulopalatopharyngoplasty (H-UPPP) can improve FMD.

Objective: To evaluate FMD in patients with moderate or severe OSAS and observe the effect of H-UPPP on FMD in these patients.

Methods: Forty-nine patients who were first diagnosed with moderate or severe OSAS by polysomnography (PSG) and had no other diseases served as the experimental group, and 35 individuals with normal PSG as the control group.

Cancer stem-like side population cells in the human nasopharyngeal carcinoma cell line cne-2 possess epithelial mesenchymal transition properties in association with metastasis.

It has been recently reported that side population (SP) cells in nasopharyngeal carcinoma (NPC) cell lines display characteristics of cancer stem-like cells. However, the biological behavior and the significance of these cells for NPC progression remain unclear. In this study, we isolated SP cells from the NPC cell line CNE-2 by flow cytometry and investigated their biological characteristics.

[The effects of the plastic operation on upper respiratory tract at one-stage for patients with OSAHS through the assessment of 3-D reconstruction of CT images].

Objective: The method of three-dimensional reconstruction of CT images is adopted to evaluate the effects of the plastic operation on upper respiratory tract at one-stage (stage one) for patients with multi-level obstructive sleep apnea hypopnea syndrome.

Method: Polysomnography is used to diagnose patients with OSAHS. The obstruction parts of patients with OSAHS are determined by CT and endoscopy examinations in the upper air way.

[Computerized three-dimensional reconstruction of cochlear microstructures].

Objective: To explore an useful and practical method for three-dimensional reconstruction of cochlear microstructure.

Method: A digital image dataset of magnified forty times of human cochlear tissue can be get from the serial sections of transverse cedukol images of male cadavers temporal bone. Photoshop 6.

[Quantitative interpretation of dexamethasone pharmacokinetics in human inner ear perilymph using computer simulations].

Objective: To study the dexamethasone pharmacokinetics of human inner ear perilymph under different drug administration using computer simulations.

Method: The dexamethasone pharmacokinetics in guinea pigs inner ear perilymph under an intratympanic application with high-performance liquid chromatography. Dexamethasone pharmacokinetics in the guinea pigs cochlear fluid were simulated with a computer model, the Washington University Cochlear Fluids Simulator, version 1.

[Effect of silencing VEGF, c-myc, survivin and hTERT by multiple shRNA expression vectors on the xenografted human nasopharyngeal carcinoma in nude mice].

Objective: To investigate the effects of multiple short hairpin (shRNA) expression vectors, targeting VEGF, c-myc, survivin and hTERT, genes on the xenografted human nasopharyngeal carcinoma (CNE-2Z) in nude mice.

Methods: The shRNA expression vectors were constructed and subsequently transfected by direct injections into the tumors formed by CNE-2Z cells implanted in nude mice. The expressions of the targeted genes in tumor tissues and the apoptosis of tumor cells were evaluated.

[Effects of RNA interference targeting four different genes on the growth and proliferation of nasopharyngeal carcinoma CNE-2Z cells].

Objective: To explore the effects of RNA interference (RNAi) targeting four different genes (VEGF, c-myc, survivin, hTERT) on the growth and proliferation of nasopharyngeal carcinoma (NPC) CNE-2Z cells.

Methods: Plasmid-1 targeted all four genes, plasmid-2, 3, 4 and 5 targeted VEGF, c-myc, survivin and hTERT respectively. These plasmids were transfected separately into human NPC CNE-2Z cells and xenograft tumors in nude mice.

Stem cell and peripheral nerve injury and repair.

Peripheral motor nerve injuries are a significant source of morbidity. Neural stem cells (NSCs), a group of relatively primitive cells, possess self-renewal ability and multidifferentiation potential. NSCs may be successfully separated from the human embryo and central nervous system (CNS) and differentiated into mature neurons and gliacytes by in vitro induction or transplantation into the body and may be differentiated into Schwann-like cells under specific conditions.

[Effects of sodium valproate on proliferation and apoptosis of Hep-2 cells and associated mechanism].

Objective: To observe the effect of sodium valproate (VPA) on proliferation and apoptosis of human laryngeal carcinoma Hep-2 cells and its associated mechanism.

Method: Methabenzthiazuron (MTT) was used to observe the proliferation of human laryngeal carcinoma Hep-2 cells treated with various concentrations of VPA at different times. Flow cytometry(FCM) and RT-PCR were used to measure the apoptosis rate and the expression of Survivin mRNA in the Hep-2 cells treated with VPA at 3 mmol/L for different times.

[Clinical features of parathyroid space-occupying lesions].

Objective: To investigate the clinical manifestation, diagnosis, treatment of parathyroid occupying lesions.

Methods: The clinical data of 42 patients with parathyroid occupying lesions were retrospectively analyzed, including the clinical symptoms and signs, laboratory results, pathologic and imaging results and treatment.

Results: The number of males and females were 8 and 34, with females: males ratio being 1:5.

[Clinical pathology and anatomy features of parapharyngeal region neoplasms: a report of 69 cases].

Objective: To analyze the relationship between clinical pathology and anatomy features of primary neoplasms in the parapharyngeal regions.

Method: A total of 69 cases of primary neoplasms in the parapharyngeal regions were included. Fifty-eight cases were benign, and 11 cases were malignancy.