Failure characteristics and control technology of surrounding rock during roadway passing collapse column.

The collapse column may result in failure of the surrounding rock during roadway excavation. It is of considerable importance to elucidate the failure characteristics and develop the corresponding control technologies for the surrounding rock. A discrete fracture mesh (DFN) and a strain-softening constitutive model are employed to simulate the properties of a collapse column.

Modelling of enhanced gas extraction in low permeability coal seam by controllable shock wave fracturing.

The controlled shock wave (CSW) fracturing is an effective method for enhancing permeability of coal seam to promote gas extraction. Based on Fick's law, Darcy's law, the ideal gas law and the Langmuir equation, a damage-seepage-deformation coupling mathematical model of CSW fracturing in coal seam combined with the maximum tensile stress and the Mohr-Coulomb criterion is established. This model is implemented into COMSOL Multiphysics to simulate the coal seam CSW fracturing and subsequent gas extraction.

Integrated transcriptome and microRNA analysis reveals molecular responses to high-temperature stress in the liver of American shad (Alosa sapidissima).

Background: Fish reproduction, development and growth are directly affected by temperature, investigating the regulatory mechanisms behind high temperature stress is helpful to construct a finer molecular network. In this study, we systematically analyzed the transcriptome and miRNA information of American shad (Alosa sapidissima) liver tissues at different cultivation temperatures of 24 ℃ (Low), 27 ℃ (Mid) and 30 ℃ (High) based on a high-throughput sequencing platform.

Results: The results showed that there were 1594 differentially expressed genes (DEGs) and 660 differentially expressed miRNAs (DEMs) in the LowLi vs.

Proteomics and metabolomics analysis of American shad (Alosa sapidissima) liver responses to heat stress.

The dramatic changes in the global climate pose a major threat to the survival of many organisms, including fish. To date, the regulatory mechanisms behind the physiological responses of fish to temperature changes have been studied, and a comprehensive analysis of the regulatory mechanisms of temperature tolerance will help to propose effective strategies for fish to cope with global warming. In this study, we investigated the expression profiles of proteins and metabolites in liver tissues of American shad (Alosa sapidissima) corresponding to different water temperatures (24 °C, 27 °C and 30 °C) at various times (1-month intervals) under natural culture conditions.

Molecular characterization and function of hif1a and fih1 in response to acute thermal stress in American shad (Alosa sapidissima).

In order to evaluate the function of hypoxia-inducible factor-1 alpha (hif1α) and factor inhibiting hif1α (fih1) in response to thermal stress, we first conducted a functional analysis of A. sapidissima hif1α and fih1, and determined hif1α and fih1 expressions in different tissues in response to thermal stress based on identified housekeeping genes (HKGs). The results showed that hif1α and fih1 were mainly located in the nucleus and cytoplasm.

High-temperature stress response: Insights into the molecular regulation of American shad (Alosa sapidissima) using a multi-omics approach.

High temperature is an important abiotic stressor that limits the survival and growth of aquatic organisms. American shad (Alosa sapidissima), a migratory fish suitable for culturing at low temperatures, is known for its delicious taste and thus has high economic value. Studies concerning changes in A.

Research on main influencing factors and complete support technology for dynamic pressure and large deformation roadway.

To determine the main factors influencing dynamic pressure and large deformation roadways, a targeted set of support technologies was designed. The 2603 air inlet roadway of the Zhangcun coal mine in Lu'an, Shanxi Province, was taken as an example. The influence of the Wenwangshan South normal fault and in situ stress field on the dynamic pressure roadway was analyzed theoretically, and the main factors influencing this dynamic pressure and large deformation roadway under natural geological conditions were determined.

microRNA regulation of skin pigmentation in golden-back mutant of crucian carp from a rice-fish integrated farming system.

Background: MicroRNAs (miRNAs) are endogenous small non-coding RNAs (21-25 nucleotides) that act as essential components of several biological processes. Golden-back crucian carp (GBCrC, Carassius auratus) is a naturally mutant species of carp that has two distinct body skin color types (golden and greenish-grey), making it an excellent model for research on the genetic basis of pigmentation. Here, we performed small RNA (sRNA) analysis on the two different skin colors via Illumina sequencing.

Supporting optimization of thick seam roadway with top coal based on orthogonal matrix analysis.

Aiming at the problem of large deformation and difficulty in surrounding rock control of the top coal roadway in thick seam, theoretical analysis, theoretical analysis, numerical simulation, orthogonal matrix analysis and other methods were used to study the roof deformation and support parameter optimization of the top coal roadway in thick seam. Firstly, the structural model and roof mechanical model of the top coal roadway in thick seam were established, and the deformation coefficient T was defined based on the relationship between curvature radius and bending moment, maximum bending moment and ultimate tensile strength of beam. According to the ratio of deformation rate between T and beam to determine the roof deformation mode of top coal roadway, the discriminant conditions of roadway roof stability under two deformation conditions are obtained.

Bolt-Cable-Mesh Integrated Support Technology for Water Drenching Roadway in Thick Coal Seam.

Aiming at the problems of large deformation and difficult control of the mining roadway water drenching in a thick coal seam, the principle of double-arch zoning cooperative surrounding rock control is studied by using the combined method of theoretical analysis, numerical simulation, and industrial experiment. The water-rock interaction of the surrounding rock of water-drenching roadway is proposed, taking into account the damage to the mechanical parameters of the surrounding rock caused by soaking water. The water-mechanical-damage coupling model for surrounding rock of a coal seam roadway is constructed, and is numerically solved by the code development using the FISH language.

Studies on the molecular level changes and potential resistance mechanism of under temperature stimulation.

In this study, we used transcriptome and proteome technology to analyze molecular level changes in tissues of cultured at high temperature (HT) and low temperature (LT). We also screened for specific anti-stress genes and proteins and evaluated the relationships between them. We identified 201,803 unigenes and 10,623 proteins.

Molecular and functional analysis of the microphthalmia-associated transcription factor (mitf) gene duplicates in red tilapia.

In vertebrates, the microphthalmia-associated transcription factor (mitf) is at the hub of the melanin synthesis regulation network. However, little information is known about its molecular characterization, expression, location, or function in skin color differentiation and variation of red tilapia. The full-length cDNA sequences (1977 bp and 1999 bp) of mitfa and mitfb, encoding polypeptides of 491 and 514 amino acids, were effectively identified from red tilapia in this study.

Transcriptome analysis of skin color variation during and after overwintering of Malaysian red tilapia.

The commercial value of red tilapia is hampered by variations in skin color during overwintering. In this study, three types of skin of red tilapia, including the skin remained pink color during and after overwintering (P), the skin changed from pink color to black color during overwintering and remained black color after overwintering (P-B), and the skin changed from pink color to black color during overwintering but recovered to pink color when the temperature rose after overwintering (P-B-P), were used to analyze their molecular mechanisms of color variation. The transcriptome results revealed that the P, P-B, and P-B-P libraries had 43, 42, and 43 million clean reads, respectively.

Dynamic Expression and Gene Regulation of MicroRNAs During Bighead Carp () Early Development.

The early development of fish is regulated through dynamic and complex mechanisms involving the regulation of various genes. Many genes are subjected to post-transcriptional regulation by microRNAs (miRNAs). In the Chinese aquaculture industry, the native species bighead carp () is important.

Comparative analysis of the gut microbiota in bighead carp under different culture patterns.

Aims: To investigate the phylogenetic composition and functional potential of bighead carp (Hypophthalmichthys nobilis) gut microbiome in two rearing patterns (bighead carp polycultured with Oreochromis niloticus in pond A and bighead carp polycultured with Cyprinus carpio in pond B, respectively), as well as the changes of plankton in the cultured water at four different time points.

Methods And Results: The intestinal contents were sequenced using Illumina HiSeq of bacterial 16S rRNA. Cyanophyta and Chlorophyta were the prevalent phytoplankton in the water, whereas Rotifers and Protozoa were the predominant zooplankton.

Evolution and genetics of bighead and silver carps: Native population conservation versus invasive species control.

Bighead carp () and silver carp (, collectively called bigheaded carps, are cyprinids native mainly to China and have been introduced to over 70 countries. Paleontological and molecular phylogenetic analyses demonstrate bighead and silver carps originated from the Yangtze-Huanghe River basins and modern populations may have derived from the secondary contact of geographically isolated fish during the last glacial events. Significant genetic differences are found among populations of native rivers (Yangtze, Pearl, and Amur) as well as introduced/invasive environments (Mississippi R.

MicroRNA-206 Regulation of Skin Pigmentation in Koi Carp ( L.).

MicroRNAs (miRNAs) are ∼22 nucleotide non-coding RNA molecules that act as crucial roles in plenty of biological processes. However, the molecular and cellular mechanisms of miRNAs to regulate skin color differentiation and pigmentation in fish have not been fully understood. Herein, we revealed that miR-206, a skin-enriched miRNA, regulates melanocortin 1 receptor (, a key regulator of melanogenesis) expression by binding to its 3'-untranslated (UTR) region through bioinformatics and luciferase reporter assay in koi carp ( L.

Dynamic transcriptome sequencing and analysis during early development in the bighead carp (Hypophthalmichthys nobilis).

Background: Early development is a key process of the life history of fish. However, the relationship between the transcriptome and the dynamic regulation of early development is still uncharacterized in the bighead carp (Hypophthalmichthys nobilis). In the present study, we performed transcriptome analysis of six development stages in H.

Integrated analysis of long non-coding RNA and mRNA expression in different colored skin of koi carp.

Background: Long non-coding RNAs (lncRNAs) perform crucial roles in biological process involving complex mechanisms. However, information regarding their abundance, characteristics and potential functions linked to fish skin color is limited. Herein, Illumina sequencing and bioinformatics were conducted on black, white, and red skin of Koi carp (Cyprinus carpio L.