LINC00963 Represses Osteogenic Differentiation of hBMSCs via the miR-10b-5p/RAP2A/AKT Axis.

Osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) is important for human bone formation. Long non-coding RNAs (lncRNAs) are critical regulators in osteogenic differentiation. This study aimed to explore the function and mechanisms of long intergenic non-protein coding RNA 963 (LINC00963) in affecting osteogenesis.

Effects of the lncRNA MALAT1 gene region rs664589 site mutation on acute myocardial infarction in Chinese Han.

We aimed to study the association between the non-coding region of the lncRNA MALAT1 gene, the non-coding region rs664589 C>G variant, and the risk of acute myocardial infarction (AMI) in the Chinese Han population. 165 NSTEMI and 135 STEMI patients were enrolled in the study. An additional 150 healthy individuals were enrolled as the controls.

Ultrasound Microbubble Delivery Targeting Intraplaque Neovascularization Inhibits Atherosclerotic Plaque in an APOE-deficient Mouse Model.

Background/aim: Intraplaque neovascularization is often associated with plaque formation, development and instability, and clinical symptoms in atherosclerosis. The aim of the present study was to investigate a new strategy for treating athrosclerosis by ultrasound-targeted microbubble delivery (UTMD) targeting intraplaque neovascularization in an APOE-deficient mouse model of atherosclerosis.

Materials And Methods: A mouse model of atherosclerosis was induced by feeding Apoe mice a hypercholesterolemic diet and was verified with hematoxylin and eosin staining and intercellular adhesion molecule 1 (ICAM-1) expression.

[Value of serum soluble lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) and LOX-1 mRNA in peripheral mononuclear cells in early diagnosis of acute coronary syndrome].

Objective: To assess the value of serum soluble lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) levels and LOX-1 mRNA expression in peripheral blood mononuclear cells in early diagnosis of acute coronary syndrome (ACS).

Methods: Enzyme-linked immunosorbent assay was used to detect the levels of plasma ox-LDL and LOX-1 in 95 patients with ACS, 60 with stable angina pectoris (SAP) and 40 normal control subjects. The expression of LOX-1 mRNA in peripheral blood mononuclear cells was detected by RT-PCR in the 3 groups.

[Clinical utilization of veiled incision and sternocleidomastoid flap in parotidectomy of parotid benign tumors].

Purpose: To describe a modified technique of parotidectomy using face-lift approach and sternocleidomastoid flap.

Methods: Forty-six patients were divided into two groups; group 1 (23 cases) using veiled incision and sternocleidomastoid flap, group 2 (23 cases) using traditional incision (Blair's approach) without sternocleidomastoid flap. Postoperative complications included temporary facial paralysis and salivary fistula.

[p38 MAPK mediates high glucose-induced renal tubular epithelial-mesenchymal transition.].

The aim of the present study was to investigate the role of p38 MAPK in the renal tubular epithelial-mesenchymal transition (TEMT) induced by high glucose. In in vivo study, the rats were randomly divided into control (C), diabetes mellitus (DM) and insulin-treated DM groups. Immunohistochemical staining and Western blot were employed to determine the expression of p38 MAPK and p-p38 MAPK protein in renal cortex of rats.

[The enhancement and inhibition of lucigenin chemiluminescence by phenol and aniline derivatives].

The effect of phenol and aniline derivatives on the lucigenin-H2O2-Co2+ chemiluminescence (CL) at different pH was studied. In NaHCO-Na2CO3 medium, some compounds enhanced the lucigenin CL while others inhibited the lucigenin CL. In NaOH medium, all tested compounds inhibited the lucigenin CL.

[Transforming growth factor-β1 and Snail1 mediate tubular epithelial-mesenchymal transition in diabetic rats].

The present study was aimed to explore the expressions of transforming growth factor-β1 (TGF-β1) and Snail1 in renal tissues of diabetic rats, and their role in tubular epithelial-mesenchymal transition (TEMT). Induced diabetic rats were randomly divided into 2-, 4-, 8-, 12-, 16-, 20-, 24-week and 16wA, 20wA, 24wA groups. The rats in 16wA, 20wA and 24wA groups were treated with insulin to control blood glucose to the normal level from the 13th week.

[Alprazolam use improves psychological status and reduces hospitalization costs in patients with paroxysmal supraventricular tachycardia underwent radiofrequency catheter ablation].

Objective: To evaluate the effect of alprazolam use on psychological status and hospitalization cost in patient with paroxysmal supraventricular tachycardia underwent electrophysiology studies or radiofrequency catheter ablation.

Methods: In this prospective, randomized, double-blind, placebo-controlled study, 142 inpatients [77 males, mean age (43.1 +/- 14.

Chemiluminescent reactions induced by gold nanoparticles.

The reaction of gold nanoparticles with a potassium periodate-sodium hydroxide-carbonate system undergoes chemiluminescence with three emission bands at 380-390, 430-450, and 490-500 nm, respectively. It was found that the light intensity increased linearly with the concentration of the gold nanoparticles, and the CL intensity increased dramatically when the citrate ions on the nanoparticle surface were replaced by SCN(-). The shape, size, and oxidation state of gold nanoparticles after the chemiluminescent reaction were characterized by UV-visible absorption spectrometry, transmission electron microscopy (TEM), and X-ray photoelectron spectrometry (XPS).

Chemiluminescence accompanied by the reaction of gold nanoparticles with potassium permanganate.

It was found that potassium permanganate (KMnO(4)) could react with gold nanoparticles in a strong acid medium to generate particle size-dependent chemiluminescence (CL). For gold nanoparticles with the size of 2.6 or 6.

Light emission of gold nanoparticles induced by the reaction of bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide.

Light emission at approximately 415 nm was observed for gold particles with diameters of 2.6-6.0 nm dispersed in a solution containing bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide.

Effect of pH on inhibition and enhancement of luminol-H2O2-Co2+ chemiluminescence by phenolic compounds and amino acids.

The effect of pH on inhibition and enhancement of luminol-H2O2-Co2+ chemiluminescence (CL) by 18 phenolic compounds and 20 amino acids was studied. It was found that most of the tested compounds showed an inhibiting effect at lower pH and an enhancing effect at higher pH. At a midrange pH, for some phenolic compounds with two ortho-position -OH, both an inhibiting and an enhancing peak were simultaneously observed.