rare variant augments aortic growth and induces cardiac hypertrophy and heart failure with pressure overload.

Smooth muscle cell-specific myosin heavy chain, encoded by , is selectively expressed in smooth muscle cells (s). Pathogenic variants in predispose to a number of disorders, including heritable thoracic aortic disease associated with patent ductus arteriosus, visceral myopathy, and megacystis-microcolon-intestinal hypoperistalsis syndrome. Rare variants of uncertain significance occur throughout the gene, including p.

Hippo-deficient cardiac fibroblasts differentiate into osteochondroprogenitors.

Cardiac fibrosis, a common pathophysiology associated with various heart diseases, occurs from the excess deposition of extracellular matrix (ECM) . Cardiac fibroblasts (CFs) are the primary cells that produce, degrade, and remodel ECM during homeostasis and tissue repair . Upon injury, CFs gain plasticity to differentiate into myofibroblasts and adipocyte-like and osteoblast-like cells, promoting fibrosis and impairing heart function .

A prospective comparison of a modified miniaturised hand-held epifluorescence microscope and touch imprint cytology for evaluation of axillary sentinel lymph nodes intraoperatively in breast cancer patients.

Background: The management of axillary lymph nodes in early-stage breast cancer patients has changed considerably, with the primary focus shifting from the examination of sentinel lymph nodes (SLNs) to toward the detection of all macro-metastases. However, current methods, such as touch imprint cytology (TIC) and frozen sections, are inadequate for clinical needs. To address this issue, we proposed a novel miniaturised epifluorescence widefield microscope (MEW-M) to assess SLN status intraoperatively for improved diagnostic efficiency.

Emerging Signaling Regulation of Sinoatrial Node Dysfunction.

Purpose Of Review: The sinoatrial node (SAN), the natural pacemaker of the heart, is responsible for generating electrical impulses and initiating each heartbeat. Sinoatrial node dysfunction (SND) causes various arrhythmias such as sinus arrest, SAN block, and tachycardia/bradycardia syndrome. Unraveling the underlying mechanisms of SND is of paramount importance in the pursuit of developing effective therapeutic strategies for patients with SND.

DeepMiceTL: a deep transfer learning based prediction of mice cardiac conduction diseases using early electrocardiograms.

Cardiac conduction disease is a major cause of morbidity and mortality worldwide. There is considerable clinical significance and an emerging need of early detection of these diseases for preventive treatment success before more severe arrhythmias occur. However, developing such early screening tools is challenging due to the lack of early electrocardiograms (ECGs) before symptoms occur in patients.

Exploring the role of sphingolipid-related genes in clinical outcomes of breast cancer.

Background: Despite tremendous advances in cancer research, breast cancer (BC) remains a major health concern and is the most common cancer affecting women worldwide. Breast cancer is a highly heterogeneous cancer with potentially aggressive and complex biology, and precision treatment for specific subtypes may improve survival in breast cancer patients. Sphingolipids are important components of lipids that play a key role in the growth and death of tumor cells and are increasingly the subject of new anti-cancer therapies.

Integrating single-cell RNA-seq and bulk RNA-seq to construct prognostic signatures to explore the role of glutamine metabolism in breast cancer.

Background: Although breast cancer (BC) treatment has entered the era of precision therapy, the prognosis is good in the case of comprehensive multimodal treatment such as neoadjuvant, endocrine, and targeted therapy. However, due to its high heterogeneity, some patients still cannot benefit from conventional treatment and have poor survival prognoses. Amino acids and their metabolites affect tumor development, alter the tumor microenvironment, play an increasingly obvious role in immune response and regulation of immune cell function, and are involved in acquired and innate immune regulation; therefore, amino acid metabolism is receiving increasing attention.

Luminescence and energy transfer of single-phase white-emitting phosphor BaMg(PO):Ce, Eu for white LEDs.

A series of BaMg(PO):Ce, Eu phosphors were synthesized by the traditional high temperature solid-state method, and the crystal structures and luminescence properties of the samples were discussed systematically. The energy transfer from Ce to Eu in BaMg(PO) was proved to be of resonant type a dipole-dipole interaction mechanism. With a precisely controlled relative proportion of Ce/Eu, the emission color of the samples can vary from blue (0.

Research on partially coherent spatial light interference microscopy.

Based on partial coherence theory, this study rigorously deduces the principle of spatial light interference microscopy (SLIM) and improves the calculation method of SLIM. The main problem we found with SLIM is that it simply defaults the phase of the direct light to 0. To address this problem, we propose and experimentally demonstrate a double four-step phase shift method.

Hippo-Yap Signaling Maintains Sinoatrial Node Homeostasis.

Background: The sinoatrial node (SAN) functions as the pacemaker of the heart, initiating rhythmic heartbeats. Despite its importance, the SAN is one of the most poorly understood cardiac entities because of its small size and complex composition and function. The Hippo signaling pathway is a molecular signaling pathway fundamental to heart development and regeneration.

Yap and Taz promote osteogenesis and prevent chondrogenesis in neural crest cells in vitro and in vivo.

Neural crest cells (NCCs) are multipotent stem cells that can differentiate into multiple cell types, including the osteoblasts and chondrocytes, and constitute most of the craniofacial skeleton. Here, we show through in vitro and in vivo studies that the transcriptional regulators Yap and Taz have redundant functions as key determinants of the specification and differentiation of NCCs into osteoblasts or chondrocytes. Primary and cultured NCCs deficient in and switched from osteogenesis to chondrogenesis, and NCC-specific deficiency for and resulted in bone loss and ectopic cartilage in mice.

Identification of expression as a novel prognostic biomarker in breast cancer correlated with immune infiltration.

has been identified as a prognostic biomarker in many neoplasms, but its role in breast cancer (BC) has not been fully explained. We aimed to investigate the molecular mechanism and prognostic value of in BC. expression profile was extracted from TIMER, Oncomine, UALCAN databases, and verified by the Gene Expression Omnibus (GEO) database and quantitative real-time polymerase chain reaction (qRT-PCR).

Optimal Selection of Imaging Examination for Lymph Node Detection of Breast Cancer With Different Molecular Subtypes.

Objective: Axillary lymph node management is an important part of breast cancer surgery and the accuracy of preoperative imaging evaluation can provide adequate information to guide operation. Different molecular subtypes of breast cancer have distinct imaging characteristics. This article was aimed to evaluate the predictive ability of imaging methods in accessing the status of axillary lymph node in different molecular subtypes.

Comparative analysis of transient receptor potential channel 5 opposite strand-induced gene expression patterns and protein-protein interactions in triple-negative breast cancer.

In patients with triple-negative breast cancer (TNBC), high tumour mutation burden and aberrant oncogene expression profiles are some of the causes of poor prognosis. Therefore, it is necessary to identify aberrantly expressed oncogenes, since they have the potential to serve as therapeutic targets. Transient receptor potential channel 5 opposite strand (TRPC5OS) has been previously shown to function as a novel tumour inducer.

TRPC5OS induces tumorigenesis by increasing ENO1-mediated glucose uptake in breast cancer.

Breast cancer is the most common malignant tumor worldwide and the leading cause of cancer-related deaths in female. Metabolic reprogramming plays critical roles in breast tumorigenesis and induces enhanced glucose uptake and glycolysis. TRPC5OS is encoded by short transient receptor potential channel 5 opposite strand, and predicted to correlate with tumor metabolic reprogramming.

Recent progress of effect of crystal structure on luminescence properties of Ce-Eu Co-doped phosphors.

Currently, the mechanism of Ce-Eu ET is frequently used to obtain color adjustable or white phosphors. Correspondingly, the ET efficiency from Ce to Eu becomes an important indication of the luminescent properties of phosphors. However, the ET efficiency calculated using the formula does not always match the emission spectra; the transmission efficiency of Ce is high, but the emission efficiency of Eu is low, depending on our investigation results.

Achievement of narrow-band blue-emitting phosphors KScSr Ca SiO:Bi by the migration of luminescence centers.

In recent years, efforts have been made to develop narrow-band emission phosphors with excellent performance. Herein, a series of KScSr Ca SiO:0.07Bi narrow-band phosphors were synthesized by a co-substitution method, and the crystal structure, the occupancy of activated ions and luminescence properties were studied in detail.

Achieving the potential multifunctional near-infrared materials CaIn Ga GeO:Cr using a solid state method.

Near-infrared spectroscopy is developing rapidly in the fields of human detection and food analysis due to its fast response and non-invasive characteristics. Herein, we report the novel near-infrared garnet-type CaInGeO:Cr and CaIn Ga GeO:0.07Cr phosphors, in which there are two crystallographic sites (CaO, InO) that can be substituted by Cr, and cation regulation engineering for In is utilized to tune the luminescence properties.

The use of trastuzumab affected by health insurance policy in Jiangsu Province of China.

Background: Breast cancer recurrence and mortality have been shown to decrease after trastuzumab treatment in human epidermal growth factor 2 (HER2)-positive early-stage breast cancer (EBC) patients. In Jiangsu Province, trastuzumab has been subsidized for patients with HER2-positive EBC since 2013. Several studies showed that Jiangsu was one of the provinces with the highest rates of adjuvant trastuzumab therapy.

Characteristics of metastasis and survival between male and female breast cancer with different molecular subtypes: A population-based observational study.

Objective: Male breast cancer (BC) is a rare disease, having different clinicopathological features and survival outcomes from female patients. The aim of this research was to, combine with molecular subtypes, analyze the metastatic patterns, and prognosis between male and female patients, and to determine whether the gender was the independent prognostic factor for BC.

Methods: Data used in this study were acquired from the SEER database from 2010 to 2016.

HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling.

RIP1 has emerged as a master regulator in TNFα signaling that controls two distinct cellular fates: cell survival versus programmed cell death. Because the default response of most cells to TNFα is NF-κB–mediated inflammation and survival, a specific mechanism must exist to control the divergence of signaling outcome. Here, we identify HSPA13 as a transcription-independent checkpoint to modulate the role of RIP1 in TNFα signaling.

Bmp Signaling Regulates Hand1 in a Dose-Dependent Manner during Heart Development.

The bone morphogenetic protein (Bmp) signaling pathway and the basic helix-loop-helix (bHLH) transcription factor Hand1 are known key regulators of cardiac development. In this study, we investigated the Bmp signaling regulation of during cardiac outflow tract (OFT) development. In and loss-of-function embryos with varying levels of in the heart, is sensitively decreased in response to the dose of expression.

The Cardiac Neural Crest Cells in Heart Development and Congenital Heart Defects.

The neural crest (NC) is a multipotent and temporarily migratory cell population stemming from the dorsal neural tube during vertebrate embryogenesis. Cardiac neural crest cells (NCCs), a specified subpopulation of the NC, are vital for normal cardiovascular development, as they significantly contribute to the pharyngeal arch arteries, the developing cardiac outflow tract (OFT), cardiac valves, and interventricular septum. Various signaling pathways are shown to orchestrate the proper migration, compaction, and differentiation of cardiac NCCs during cardiovascular development.

Crumbs proteins stabilize the cone mosaics of photoreceptors and improve vision in zebrafish.

Although the unique organization of vertebrate cone mosaics was first described long ago, both their underlying molecular basis and physiological significance are largely unknown. Here, we demonstrate that Crumbs proteins, the key regulators of epithelial apical polarity, establish the planar cellular polarity of photoreceptors in zebrafish. Via heterophilic Crb2a-Crb2b interactions, the apicobasal polarity protein Crb2b restricts the asymmetric planar distribution of Crb2a in photoreceptors.