Publications by authors named "Minghui Pu"
Synth Syst Biotechnol
June 2023
Article Synopsis
- d-Mannose is a functional sugar with health benefits, and there’s a growing market for low-calorie sweeteners, but the enzyme d-lyxose isomerase (d-LIase) struggles with thermal stability, limiting its use.
- Researchers used computer-aided design tools to create mutants of d-LIase that have better thermostability, with one promising mutant (M5) demonstrating significantly enhanced stability and efficiency compared to the original enzyme.
- Molecular dynamics simulations revealed that the improved stability of mutant M5 is due to a more rigid protein structure, new hydrogen bonds, and changes in electrostatic properties, making it a potential candidate for industrial production of d-mannose from d-fructose.
