Alteration or abnormal activation of RTKs have been recurrently observed and recognized as an important driving factor in the progression of many human cancers. Ferroptosis, an iron-dependent form of regulated necrosis triggered by the accumulation of lethal lipid peroxides on cell membranes, has been implicated in various tumor types. Here we reported that oncogenic RTKs/RAS/RAF/c-Myc axis promotes cancer cells to ferroptosis.
View Article and Find Full Text PDFExtrahepatic cholangiocarcinoma (ECC), a highly malignant type of cancer with increasing incidence, has a poor prognosis due to limited treatment options. Based on genomic analysis of ECC patient samples, here we report that aldo-keto reductase family 1 member C1 (AKR1C1) is highly expressed in human ECC tissues and closely associated with ECC progression and poor prognosis. Intriguingly, we show that inducible AKR1C1 knockdown triggers ECC cells to undergo ferroptosis.
View Article and Find Full Text PDFJ Asian Nat Prod Res
January 2025
Twelve monoterpene indole glycoalkaloids, comprising of three new ones, 19--rhynchophylloside A (), 7--rhynchophylloside A (), and 7--anthocephalusine A (), were isolated from the hook-bearing branches of . The structures and absolute configurations of were elucidated by analysis of MS, NMR, ECD, and single-crystal X-ray diffraction or TDDFT-ECD calculations. Glycoalkaloids and showed significant immunosuppressive activity against the proliferation of B lymphocyte induced by LPS with broad selective index.
View Article and Find Full Text PDFThe negative regulation on neurogenesis has been implicated in fluoride neurotoxicity, while the evidence is limited. To explore whether fluoride interferes with neurogenesis via the Notch1 signaling and the potential alleviation effects of carvacrol (CAR), we conducted in vivo and in vitro experiments, as well as epidemiological analyses in this study. The results showed that urinary fluoride levels and circulating Notch1 levels were associated with IQ levels in boys.
View Article and Find Full Text PDFCapillary electrophoresis with capacitively coupled contactless conductivity detection (CE-CD) has proven to be an efficient technique for the separation and detection of charged inorganic, organic, and biochemical analytes. It offers several advantages, including cost-effectiveness, nanoliter injection volume, short analysis time, good separation efficiency, suitability for miniaturization, and portability. However, the routine determination of common inorganic cations (NH, K, Na, Ca, Mg, and Li) and inorganic anions (F, Cl, Br, NO, NO, PO, and SO) in water quality monitoring typically exhibits limits of detection of about 0.
View Article and Find Full Text PDFCorrection for 'A compact and high-performance setup of capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-CD)' by Lin Li , , 2024, https://doi.org/10.1039/d4an00354c.
View Article and Find Full Text PDFCapillary electrophoresis with capacitively coupled contactless conductivity detection (CE-CD) has the advantages of high throughput (simultaneous detection of multiple ions), high separation efficiency (higher than 10 theoretical plates) and rapid analysis capability (less than 5 min for common inorganic ions). A compact CE-CD system is ideal for water quality control and on-site analysis. It is suitable not only for common cations ( Na, K, Li, NH, Ca, .
View Article and Find Full Text PDFFerroptosis is an iron-dependent and lipid peroxides (LPO)-overloaded programmed damage cell death, induced by glutathione (GSH) depletion and glutathione peroxide 4 (GPX4) inactivation. However, the inadequacy of endogenous iron and reactive oxygen species (ROS) restricts the efficacy of ferroptosis. To overcome this obstacle, a near-infrared photo-responsive FeP@PEG NPs is fabricated.
View Article and Find Full Text PDFGenetically engineered macrophages (GEMs) have emerged as an appealing strategy to treat cancers, but they are largely impeded by the cell availability and technical challenges in gene transfer. Here, we develop an efficient approach to generate large-scale macrophages from human induced pluripotent stem cells (hiPSCs). Starting with 1 T150 dish of 10 hiPSCs, more than 10 mature macrophages (iMacs) could be generated within 1 month.
View Article and Find Full Text PDFEmergency myelopoiesis (EM) is essential in immune defense against pathogens for rapid replenishing of mature myeloid cells. During the EM process, a rapid cell-cycle switch from the quiescent hematopoietic stem cells (HSCs) to highly proliferative myeloid progenitors (MPs) is critical. How the rapid proliferation of MPs during EM is regulated remains poorly understood.
View Article and Find Full Text PDFBackground: Rice taste is closely associated with endosperm composition, which varies among different rice layers. Although clarifying the relationship between this difference and nutritional taste can guide rice breeding and cultivation practices, research on this topic is limited.
Results: Here, typical rice varieties having excellent and poor taste characteristics were selected to analyze the distribution characteristics and differences of their components.
The underlying mechanism of fluorosis has not been fully elucidated. The purpose of this study was to explore the mechanism of fluorosis induced by sodium fluoride (NaF) using proteomics. Six offspring rats exposed to fluoride without dental fluorosis were defined as group A, 8 offspring rats without fluoride exposure were defined as control group B, and 6 offspring rats exposed to fluoride with dental fluorosis were defined as group C.
View Article and Find Full Text PDF(TV) may have an impact on other reproductive tract infections. Studies on the connection between the infection of TV and human papillomavirus (HPV) have been inconsistent. We performed a systematic review of the relevant articles through keywords that satisfy the criteria and filtered the articles according to the inclusion and exclusion criteria.
View Article and Find Full Text PDFItch is an uncomfortable and complex sensation that elicits the desire to scratch. The nucleus accumbens (NAc) activity is important in driving sensation, motivation, and emotion. Excitatory afferents from the medial prefrontal cortex (mPFC), amygdala, and hippocampus are crucial in tuning the activity of dopamine receptor D1-expressing and D2-expressing medium spiny neurons (Drd1-MSN and Drd2-MSN) in the NAc.
View Article and Find Full Text PDFCryptosporidium parvum has gained much attention as a major cause of diarrhea in the world, particularly in those with compromised immune systems. The data currently available on how the immune system recognizes C. parvum are growing rapidly, but we lack data on the interactions among host major histocompatibility complex (MHC) diversity and parasitic T-cell epitopes.
View Article and Find Full Text PDFMitochondrial DNA B Resour
December 2022
The (Boulenger, 1900) is a small Cyprinidae fish that has a wide distribution in China. In this study, we characterized the complete mitochondrial genome of by the Illumina NovaSeq sequencing platform in Guangxi, China. The assembled mitogenome is 16,647 base pairs (bp) and consists of 13 protein-coding genes (PCGs), 22 transfer RNAs, two ribosomal RNAs, and a control region (D-loop).
View Article and Find Full Text PDFThe deposition of wax on the surface of the pipeline or the facilities is harmful to oil industries, which can cause serious safety problems and economic loss. To address this problem, a degradable glutinous rice/chitosan-phytic acid composite coating (GR/CS-PA) was prepared. The GR can serve as an adhesive, and it can also enhance the flexibility of CS-PA.
View Article and Find Full Text PDFGiven the high incidence and high mortality of cervical cancer (CC) among women in developing countries, identifying reliable biomarkers for the prediction of prognosis and therapeutic response is crucial. We constructed a prognostic signature of cuproptosis-related long non-coding RNAs (lncRNAs) as a reference for individualized clinical treatment. A total of seven cuproptosis-related lncRNAs closely related to the prognosis of patients with CC were identified and used to construct a prognostic signature via least absolute shrinkage and selection operator regression analysis in the training set.
View Article and Find Full Text PDFHerein, we describe the copper-catalyzed arylalkylation of activated alkenes via hydrogen-atom transfer and aryl migration strategy. The reaction was carried out through a radical-mediated continuous migration pathway using -fluorosulfonamides as the alkyl source. The primary, secondary, and tertiary alkyl radicals formed by intramolecular hydrogen-atom transfer proceeded smoothly.
View Article and Find Full Text PDFA general and expedient method to construct the scaffolds of 2-alkenylpyridines, through copper-catalyzed C2 alkenylation of pyridine--oxides with alkynes, has been disclosed. This protocol shows operational simplicity, good functional group compatibility and broad substrate scope.
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