Preparation of water-insoluble lignin nanoparticles by deep eutectic solvent and its application as a versatile and biocompatible support for the immobilization of α-amylase.

As one of the most abundant biopolymers, lignin is a widely available resource. However, its potential largely remains untapped, with most of it ending up as waste from industries like paper production, pulp processing, and bio-refining. The research undertaken in this study focused on the extraction of lignin from agroforestry waste using a deep eutectic solvent (DES) as a carrier for α-amylase immobilization, resulting in high stability and reusability.

Design of composite nanosupports and applications thereof in enzyme immobilization: A review.

Enzyme immobilization techniques have developed dramatically over the past several decades. Support materials are key in shaping the function of a specific immobilized enzyme. Although they have large specific surface areas and functional active sites, single-component nanomaterials and their surface chemical modification derivatives struggle to meet increasing demand.