Different viruses are abundant in aquatic ecosystems. There has been limited research on the viral communities in the upper reaches of the Yangtze River. Yellow catfish (), an important economic fish that is widely distributed in the upper reaches of the Yangtze River, was selected as the research object.
View Article and Find Full Text PDFBiosens Bioelectron
January 2022
Exosomal microRNAs (miRNAs) play a key role in cell-cell communication to regulate gene expression in target cells and have great potential as biomarkers for disease diagnosis. This paper reports an on-chip exosomal miRNA amplification and detection system for rapid analysis of exosomal miRNAs. The compact system consists of two connected flow cells for processing exosomes and detecting miRNAs, respectively.
View Article and Find Full Text PDFPleistocene climatic oscillations have greatly influenced the evolutionary histories and distribution patterns of most extant species. In this study, the phylogeographic patterns and evolutionary history of were investigated. A total of 359 individuals from 19 locations covering the species' distribution range were collected, and two mitochondrial genes (COI and Cyt ) and the recombination activating protein 1 gene (Rag1) were analyzed.
View Article and Find Full Text PDFPaper-based sensors can be exploited to develop low-cost, disposable, and rapid assays for the detection of a large variety of analytes. We report a paper-based sensor system for a point-of-care (POC) nucleic acid amplification test that can quantitatively detect multiple genes from different pathogens. The POC system combines a paper sensor chip and a portable instrument, which is built on an Internet of Things (IoT) platform.
View Article and Find Full Text PDFThe dam constructions greatly changed the hydrologic conditions in the Yangtze River, and then significantly affected the spawning activities of indigenous river fish. Monitoring the species composition of drifting eggs during spawning season is important for protection issues. In this study, we have sampled drifting fish eggs in nine locations from 2014 to 2016.
View Article and Find Full Text PDFMitochondrial DNA A DNA Mapp Seq Anal
January 2017
The complete mitogenome sequence of Glyptothorax laosensis was determined using long PCR reactions. The genome is 16 539 bp in length, including 13 typical vertebrate protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a control region. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand.
View Article and Find Full Text PDFMitochondrial DNA
June 2016
The complete mitogenome sequence of Hampala macrolepidota was determined using PCR reactions. The genome is 16,766 bp in length, including 13 typical vertebrate protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a control region. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand.
View Article and Find Full Text PDFThe mitochondrial genome of Mystacoleucus marginatus (Cypriniformes, Cyprinidae) has been sequenced. The total sequence is 16,611 bp in size with 56.67% AT content.
View Article and Find Full Text PDFThe complete mitogenome sequence of Tor sinensis was determined using long PCR reactions. The genome is 16,579 bp in length, including 13 typical vertebrate protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a control region. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand.
View Article and Find Full Text PDFThis study examined the age structure of the Loach, Homatula pycnolepis through the otolith growth rings in 204 individual specimens collected from the Xiaomengtong River of the Nujiang River (Salween River) basin in April, 2008. There were only two different age classes, 1 and 2 years of age-no 3 year olds were detected. The age structure of H.
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