The value and distribution of high-density lipoprotein subclass in patients with acute coronary syndrome.

Background: High-density lipoprotein (HDL) enhances cholesterol efflux from the arterial wall and exhibits potent anti-inflammatory and anti-atherosclerosis (AS) properties. Whether raised HDL levels will clinically benefit patients with acute coronary syndrome (ACS) and the value at which these effects will be apparent, however, is debatable. This study examined the HDL subclass distribution profile in patients with ACS.

[Studies of influences of blood glucose controlling on the changes of lipid profiles, ApoB100, ApoAI and HDL subclass of newly diagnosed type 2 diabetes].

This study was aimed to observe if the lipid profiles, apoprotein B100 (ApoB100), ApoAI, high density lipoprotein (HDL) and its subclasses could be improved by controlling the blood glucose. Fifty-three patients with newly diagnosed type 2 diabetic were divided into four groups, diet and exercise group (n = 13), continuous subcutaneous insulin infusion (CSII) group (n = 14), multiple daily insulin injection group (MDI, n = 13), and oral hypoglycaemic agents group (n = 13). Fasting blood glucose (FPG), glycated hemoglobin A1c (HbA1c), lipid profiles, ApoB100, ApoAI and HDL subclasses were measured at beginning and a month later.

Statin treatment improves plasma lipid levels but not HDL subclass distribution in patients undergoing percutaneous coronary intervention.

Despite the established efficacy of statin therapy, the risk of cardiovascular events remains high in many patients. We examined high-density lipoprotein (HDL) subclass distribution profiles among statin-treated coronary heart disease (CHD) patients undergoing percutaneous coronary intervention (PCI). Plasma HDL subclasses were measured in 85 patients with established CHD and quantified by two-dimensional gel electrophoresis and immunoblotting.

High-density lipoprotein subclass and particle size in coronary heart disease patients with or without diabetes.

Background: A higher prevalence of coronary heart disease (CHD) in people with diabetes. We investigated the high-density lipoprotein (HDL) subclass profiles and alterations of particle size in CHD patients with diabetes or without diabetes.

Methods: Plasma HDL subclasses were quantified in CHD by 1-dimensional gel electrophoresis coupled with immunodetection.

Characteristics of high-density lipoprotein subclasses distribution for subjects with desirable total cholesterol levels.

Background: To investigate alteration of high density lipoproteins (HDL) subclasses distribution in different total cholesterol (TC) levels, mainly the characteristics of HDL subclasses distribution in desirable TC levels and analyze the related mechanisms.

Methods: ApoA-I contents of plasma HDL subclasses were determined by 2-dimensional gel electrophoresis coupled with immunodetection. 486 Chinese Adults subjects were assigned to different TC groups according to the third Report of NCEP (ATP-III) guidelines.

The impact of plasma triglyceride and apolipoproteins concentrations on high-density lipoprotein subclasses distribution.

Objective: To investigate the effect of triglyceride (TG) integrates with plasma major components of apolipoproteins in HDL subclasses distribution and further elicited the TG-apolipoproteins (apos) interaction in the processes of high density lipoprotein (HDL) mature metabolic and atherosclerosis related diseases.

Methods: Contents of plasma HDL subclasses were quantities by two-dimensional gel electrophoresis associated with immunodetection in 500 Chinese subjects.

Results: Contents of preβ1-HDL, HDL3a, and apoB-100 level along with apoB-100/A-I ratio were significantly increased, whereas there was a significant reduction in the contents of HDL2, apoA-I level as well as apoC-III/C-II ratio with increased TG concentration.

The relationship between high density lipoprotein subclass profile and plasma lipids concentrations.

HDL particles possess multiple antiatherogenic activities and the identification and differentiation of individual HDL subclasses may be useful in documentation and understanding of metabolic changes of different HDL subclasses. The major plasma lipids exist and are transported in the form of lipoprotein complexes. Hence, alterations in plasma lipids levels can interfere with the composition, content, and distribution of plasma lipoprotein subclasses that affect atherosclerosis risk.

Association of the low-density lipoprotein cholesterol/high-density lipoprotein cholesterol ratio and concentrations of plasma lipids with high-density lipoprotein subclass distribution in the Chinese population.

Background: To evaluate the relationship between the low-density lipoprotein cholesterol (LDL-C)/high-density lipoprotein cholesterol (HDL-C) ratio and HDL subclass distribution and to further examine and discuss the potential impact of LDL-C and HDL-C together with TG on HDL subclass metabolism.

Results: Small-sized prebeta1-HDL, HDL3b and HDL3a increased significantly while large-sized HDL2a and HDL2b decreased significantly as the LDL-C/HDL-C ratio increased. The subjects in low HDL-C level (< 1.

[Antioxidants inhibit the oxidative modification of high density lipoproteins].

Objective: To study the role and mechanism of antioxidants on inhibiting oxidative modification of high density lipoproteins (HDL).

Methods: Freshly prepared human plasma HDL was treated by incubation with copper ion, hyperchlorite or arterial wall cells. Compared to control, the test groups were treated with addition of different concentration of butylhydroxytoluene (BHT), vitamin C and vitamin E.

Influence of apolipoproteinCII concentrations on HDL subclass distribution.

Aim: To demonstrate the interrelationship between plasma apolipoprotein(apo)CII concentrations and the pattern of high-density lipoprotein (HDL) subclass distribution.

Methods: ApolipoproteinA-I contents of plasma HDL subclasses were quantified by 2-dimensional gel electrophoresis associated with immunodetection in 504 Chinese subjects.

Results: Compared with subjects in the lowest tertile of the apoCII group, the contents of prebeta(1)- HDL, HDL(3b), and HDL(3a) were significantly higher than HDL(2a) and HDL(2b) in subjects in the middle and highest tertiles of the apoCII group.

Relationship between apolipoprotein C-III concentrations and high-density lipoprotein subclass distribution.

High-density lipoprotein (HDL) subclasses have different antiatherogenic potentials and functional properties. This work presents our findings and discussions on their metabolic implications on apolipoprotein (apo) C-III together with other apolipoprotein levels and HDL subclass distribution profile. Apolipoprotein A-I contents of plasma HDL subclasses were quantitated by 2-dimensional gel electrophoresis coupled with immunodetection in 511 subjects.

Relationship between plasma apolipoproteinB concentrations, apolipoproteinB/apolipoproeinA-I and HDL subclasses distribution.

Background: To analyze the relationship between plasma apoB100 concentrations, apoB100/apoA-I ratio and the alteration of HDL subclasses distribution.

Methods: The apoA-I contents of plasma HDL subclasses were quantitated by 2-dimensional gel electrophoresis coupled with immunodetection in 506 subjects.

Results: The subjects in the highest tertile of apoB100 groups had significantly higher small-sized prebeta(1)-HDL, however lower large-sized HDL(2a), HDL(2b) than the subjects in the lowest tertile of apoB100 group.

[Study on the protective effect of HDL subclasses against lipopolysaccharide].

Aim: To elucidate the anti-LPS effect of high-density lipoprotein (HDL) subclasses.

Methods: The pNF-kappaB-luc plasmid was transfected into HepG2 cells and the luciferase expression was obtained by the optimization of cell density, incubation time of transfected cells and stimulating concentration of LPS. The luciferase expression was examined in the cells treated with various stimulus including LPS, mixtures of HDL subclasses and LPS.

Relationship between apolipoproteins and the alteration of HDL subclasses in hyperlipidemic subjects.

Background: To elucidate the relationship between the apolipoproteins, especially apoA-I and the alteration of HDL subclasses in hyperlipidemic, HTC and HTG subjects.

Methods: ApoA-I contents of plasma HDL subclasses were quantitated by two-dimensional gel electrophoresis coupled with immunodetection in 233 normolipidemic subjects and 312 hyperlipidemic subjects (132 HTC and 180 HTG subjects). Making use of the mean +/-1 SD of apoA-I levels, we further subdivided normolipidemic, hyperlipidemic, HTC and HTG subjects into 3 subgroups, respectively.

Relationship between apolipoprotein concentrations and HDL subclasses distribution.

Alterations in plasma apolipoproteins levels can influence the composition, content, and distribution of plasma lipoproteins that affect the risk of atherosclerosis. This study assessed the relationship between plasma apolipoproteins levels, mainly apoAI, and HDL subclass distribution. The contents of plasma HDL subclasses were determined by two-dimensional gel electrophoresis coupled with immunodetection in 545 Chinese subjects.

Alterations of high-density lipoprotein subclasses in hypercholesterolemia and combined hyperlipidemia.

Background: Alterations in plasma lipid levels can influence the composition, content, and distribution of plasma lipoprotein subclasses that effect atherosclerosis risk. Hypercholesterolemia and combined hyperlipidemia are common forms of atherogenic dyslipoproteinemia. This study evaluates the alterations of high-density lipoprotein (HDL) subclasses in hypercholesterolemic and combined hyperlipidemic subjects.

Alterations of high density lipoprotein subclasses in obese subjects.

The object of this study was to investigate the characteristics of lipid metabolism in obese subjects, with particular emphasis on the alteration of HDL subclass contents and distributions. A population of 581 Chinese individuals was divided into four groups (25 underweight subjects, 288 of desirable weight, 187 overweight, and 45 obese) according to body mass index (BMI). Apoprotein A-I (apoA-I) contents of plasma HDL sub-classes were determined by 2-D gel electrophoresis associated with an immunodetection method.

Relationship between total cholesterol/high-density lipoprotein cholesterol ratio, triglyceride/high-density lipoprotein cholesterol ratio, and high-density lipoprotein subclasses.

Alterations in plasma lipid levels can influence the composition, content, and distribution of plasma lipoprotein subclasses that affect atherosclerosis risk. This study evaluated the relationship between plasma total cholesterol (TC)/high-density lipoprotein cholesterol (HDL-C) ratio, triglyceride (TG)/HDL-C ratio, and HDL subclass distribution. The apolipoprotein A-I contents of plasma HDL subclasses were quantitated by 2-dimensional gel electrophoresis coupled with immunodetection in 442 Chinese subjects.

Relationship between plasma HDL subclasses distribution and lipoprotein lipase gene HindIII polymorphism in hyperlipidemia.

Background: Different high-density lipoprotein (HDL) subclasses have distinct but interrelated metabolic functions. HDL directly influences the atherogenic process, and changes in HDL subclasses distribution may be related to the incidence and prevalence of atherosclerosis. Lipoprotein lipase (LPL) is an important enzyme for hydrolysis of triglyceride-rich lipoproteins, and its activity is positively correlated with the plasma HDL cholesterol level.

Alterations of high-density lipoprotein subclasses in endogenous hypertriglyceridemia.

Background: To investigate the alterations of high-density lipoprotein (HDL) subclasses in endogenous hypertriglyceridemic subjects.

Methods: Apolipoprotein A-I contents of plasma HDL subclasses were quantitated by 2-dimensional gel electrophoresis in 236 normolipidemic subjects (including 146 males and 90 females) and 176 endogenous hypertriglyceridemic subjects (including 103 males and 73 females).

Results: Apolipoprotein A-I contents of small-sized pre-beta1-HDL and HDL3a were significantly higher (P < .

Relationship between plasma HDL subclasses distribution and apoA-I gene polymorphisms.

Background: It is generally accepted that apolipoprotein (apo) A-I is the dominant structural apolipoprotein of HDL particles and different HDL subclasses have distinct but interrelated metabolic functions. HDL is known to directly affect the atherogenic process hence changes in HDL subclasses distribution may be related to the incidence and prevalence of atherosclerosis.

Methods: The ApoA-I contents (mg/l) of plasma HDL subclasses were determined by 2-dimensional gel electrophoresis coupled with immunodetection and apoA-I genotypes were assayed by PCR-RFLP in 307 Chinese subjects (169 males, 138 females).

Relationship between plasma lipid concentrations and HDL subclasses.

Background: It is generally accepted that different high-density lipoprotein (HDL) subclasses have distinct but interrelated metabolic functions. HDL is known to directly influence the atherogenic process and changes in HDL subclasses distribution may be related to the incidence and prevalence of atherosclerosis.

Methods: Apo-AI contents(mg/l) of plasma HDL subclasses were determined by 2-dimensional gel electrophoresis coupled with immunodetection for apo-AI.

[Association between serum lipoprotein-cholesterol levels and HDL subpopulations].

Objective: To investigate the influence of serum HDL-C or LDL-C levels on the components of serum HDL subpopulations.

Methods: Apolipoprotein (apo) A-I contents of serum HDL subpopulations in 292 subjects were determined by two-dimensional gel electrophoresis in conjunction with immunodection.

Results: With the decrease of serum HDL-C levels, the apoA-I contents of pre beta1-HDL and HDL3b increased and were significantly higher (P<0.

[Study on apoE gene polymorphism and subclasses of serum high density lipoprotein in type IV hyperlipidemia].

Objective: The aim of the study was to investigate apolipoprotein(apo) E polymorphism and its relationship with serum lipids and apolipoprotein, serum high density lipoprotein(HDL) subclasses in patients with type IV hyperlipidemia.

Methods: apoE genotype was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The subclasses of serum HDL in 103 patients with type IV hyperlipidemia and 146 normolipidemic subjects were determined by two-dimensional gel electrophoresis in conjunction with immunodetection method.

[Subclasses of serum HDL in 151 healthy adults in Chengdu].

Objective: To investigate the components of subclasses of serum HDL in healthy adults and the impact of age and gender on these components.

Methods: The components of subclasses of serum HDL in 151 healthy adults were determined by two dimensional gel electrophoresis associated with immunodetection method.

Results: The average concentration of prebeta1-HDL, prebeta2-HDL, HDL(3c), HDL(3b), HDL(3a), HDL2a, and HDL(2b) were 6.