A powerful but frequently overlooked role of thermodynamics in environmental microbiology: inspirations from anammox.

Thermodynamics has long been applied in predicting undiscovered microorganisms or analyzing energy flows in microbial metabolism, as well as evaluating microbial impacts on global element distributions. However, further development and refinement in this interdisciplinary field are still needed. This work endeavors to develop a whole-cycle framework integrating thermodynamics with microbiological studies, focusing on representative nitrogen-transforming microorganisms.

Analyzing performance and microbial mechanisms in an incineration leachate treatment after waste separation: Integrated metagenomic and metaproteomic analyses.

The absence of food waste after separation poses a significant challenge to incineration leachate treatment, as it decreases the C/N ratio and COD of leachate, greatly impacting the biological treatment process. A one-year in-situ study was systematically conducted in an incineration leachate treatment plant that experienced waste separation, focusing on the variations in carbon and nitrogen removal performance as well as the involved microbial mechanism of the "anaerobic digestion (AD) + two-stage A/O" process. Results indicated that the biodegradability of leachate significantly decreased over time, with COD concentration decreasing by 10 times and the average C/N ratio decreasing from 12.

Revealing the comprehensive impact of organic compounds on the partial nitrification-anammox system during incineration leachate treatment: metabolic hierarchy and adaptation.

Organics, as widespread pollutants in high-strength ammonia wastewater, typically exert adverse effects on the performance of partial nitrification-anammox (PNA) systems. However, the in-depth knowledge on how microbial consortia respond to these disturbances remains limited. In this study, we unveiled the evolution of complex organic matter flow and its impact on the metabolic hierarchy and adaptation of microbial consortia, employing multi-omics approaches, i.

Innovative determination of the specific anammox activity for anammox sludge from continuous flow reactors: A comparison between continuous flow test and batch test.

A novel method for measuring specific anammox activity (SAA) was proposed based on continuous flow tests to accurately determine the SAA of anammox sludge from continuous flow reactors, resolving the challenges of inaccurate SAA assessment caused by substrate shock to anammox bacteria. Results showed SAA of expanded granular sludge bed sludge via batch tests (0.101 ± 0.

Metagenomics insight into the long-term effect of ferrous ions on the mainstream anammox system.

Anaerobic ammonium oxidation (anammox) bacteria have a high requirement for iron for their growth and metabolism. However, it remains unclear whether iron supplementation can sustain the stability of mainstream anammox systems at varying temperatures. Here, we investigated the long-term effects of Fe on the mainstream anammox systems.

Sulfur autotrophic denitrification as an efficient nitrogen removals method for wastewater treatment towards lower organic requirement: A review.

The sulfur autotrophic denitrification (SADN) process is an organic-free denitrification process that utilizes reduced inorganic sulfur compounds (RISCs) as the electron donor for nitrate reduction. It has been proven to be a cost-effective and environment-friendly approach to achieving carbon neutrality in wastewater treatment plants. However, there is no consensus on whether SADN can become a dominant denitrification process to treat domestic wastewater or industrial wastewater if organic carbon is desired to be saved.

COVID-19 inhibits spermatogenesis in the testes by inducing cellular senescence.

COVID-19 (SARS-CoV-2) has been linked to organ damage in humans since its worldwide outbreak. It can also induce severe sperm damage, according to research conducted at numerous clinical institutions. However, the exact mechanism of damage is still unknown.

Oxidative stress and antioxidant mechanisms of obligate anaerobes involved in biological waste treatment processes: A review.

In-depth understanding of the molecular mechanisms and physiological consequences of oxidative stress is still limited for anaerobes. Anaerobic biotechnology has become widely accepted by the wastewater/sludge industry as a better alternative to more conventional but costly aerobic processes. However, the functional anaerobic microorganisms used in anaerobic biotechnology are frequently hampered by reactive oxygen/nitrogen species (ROS/RNS)-mediated oxidative stress caused by exposure to stressful factors (e.

A versatile control strategy based on organic carbon flow analysis for effective treatment of incineration leachate using an anammox-based process.

Anammox-based process provides an alternative for the sustainable treatment of incineration leachate that has high-load ammonium and high residual heat, but the high concentrations of organics in such leachates brought challenges for the process control. For the first time, a two-stage partial nitrification (PN)-anammox process coupled with a pre-enhanced anaerobic digestion (AD) was established to achieve efficient nitrogen removal from incineration leachate. Satisfactory nitrogen and chemical oxygen demand (COD) removal efficiencies were achieved-with the average values of 90% and 78%, respectively-despite fluctuating influent properties [1100-2000 mg-total nitrogen (TN)/L and 3800-15800 mg-COD/L].

Multidisciplinary characterization of nitrogen-removal granular sludge: A review of advances and technologies.

Nitrogen-removal granular sludge (NRGS) is a promising technology in wastewater treatment, with advantages of efficient nitrogen removal, less footprint, lower sludge production and energy consumption, and is a way for wastewater treatment plants to achieve carbon-neutrality. Aerobic granular sludge (AGS) and anammox granular sludge (AnGS) are two typical NRGS technologies that have attracted extensive attention. Mounting evidence has shown strong associations between NRGS properties and the status of NRGS systems; however, a holistic view is still missing.

Function of Fe(III)-minerals in the enhancement of anammox performance exploiting integrated network and metagenomics analyses.

Iron is a recognized physiological requirement for microorganisms but, for anaerobic ammonium oxidation (anammox) bacteria, its role extends well beyond that of a nutritional necessity. In this study, the function of two typical Fe(III)-minerals (ferrihydrite and magnetite) in anammox processes was evaluated in the absence/presence of Fe(II) by integrated network and metagenomics analyses. Results showed that Fe-(III) minerals addition increased the activity of cellular processes and pathways associated with granule formation, enabling the peak values of particle size to increase by 144% and 115%, respectively.

MiR-629-5p Promotes Prostate Cancer Development and Metastasis by Targeting AKAP13.

Prostate cancer (PCa) has become the most frequently occurring cancer among western men according to the latest report, and patients' prognosis is often poor in the event of tumor progression, therefore, many researches are devoted to exploring the molecular mechanism of PCa metastasis. MicroRNAs (miRNA) have proved to play an important role in this process. In present study, by combining clinical samples with public databases, we found that miR-629-5p increased to varying degrees in primary localized PCa tissues and metastatic PCa tissues compared with adjacent normal tissues, and bioinformatics analysis suggested that high level of miR-629-5p was related to poor prognosis.

Application of hydrophilic modified nylon fabric membrane in an anammox-membrane bioreactor: performance and fouling characteristics.

The membrane fouling is the main bottleneck hindering the wide applications of anammox-membrane bioreactor (MBR). In this study, surface-coating hydrophilic modification of the membrane using polyvinyl alcohol was applied in a granular anammox-MBR. Stable anammox performance of >77% total nitrogen removal efficiency was achieved in both original and modified MBRs, along with decreasing anammox granule size.

The Correlation between PSCA Expression and Neuroendocrine Differentiation in Prostate Cancer.

The prostate stem cell antigen (PSCA), as a predominantly prostate-specific marker, is overexpressed in most prostate cancer specimens, is positively correlated with prostate cancer androgen independence, and has the potential to be treated with castration-resistant prostate cancer (CRPC) as a gene therapy target. Using the typical androgen deprivation therapy, most tumors will progress to CRPC, as well as develop into neuroendocrine prostate cancer (NEPC) characterized by the expression of neuroendocrine markers such as enolase 2 (NSE). Our study was aimed at investigating the expressions of PSCA and NSE and the relationship between the two markers, as well as the correlation between the PSCA and NSE expressions and the clinicopathological parameters in prostate cancer specimens from 118 patients by using immunohistochemistry.

Organic compounds evolution and sludge properties variation along partial nitritation and subsequent anammox processes treating reject water.

Reject water contains complex components of organic compounds, which have significant influences on the nitrogen removal performance when treated using biological autotrophic nitrogen removal technology. In this study, a two-stage partial nitritation (PN)-anammox (floc-granule) system was established to treat reject water (COD/NH-N = 0.97 ± 0.

The m6A methylation regulator-based signature for predicting the prognosis of prostate cancer.

To construct a survival prediction signature for prostate cancer (PC) based on the RNA N6-methyladenosine (m6A) methylation regulator. This paper explores the interaction network of differentially expressed m6A RNA methylation regulators in PC by Pearson correlation analysis. Univariate Cox risk regression and LASSO regression analysis were used to construct a predictive signature of PC.

Chopper-modulated gas chromatography electroantennography enabled using high-temperature MEMS flow control device.

We report the design, fabrication and characterization of a microelectromechanical systems (MEMS) flow control device for gas chromatography (GC) with the capability of sustaining high-temperature environments. We further demonstrate the use of this new device in a novel MEMS chopper-modulated gas chromatography-electroantennography (MEMS-GC-EAG) system to identify specific volatile organic compounds (VOCs) at extremely low concentrations. The device integrates four pneumatically actuated microvalves constructed via thermocompression bonding of the polyimide membrane between two glass substrates with microstructures.

G-Fresnel smartphone spectrometer.

We report a smartphone spectrometer with nanometer resolution working in the visible range. A G-Fresnel device with the dual functionality of focusing and dispersion is used to enable miniaturization. Proof of principle application to Bradford assay of protein concentration is also demonstrated.

On-demand one-step synthesis of monodisperse functional polymeric microspheres with droplet microfluidics.

A simple and robust method for one-step synthesis of monodisperse functional polymeric microspheres was established by generation of reversed microemulsion droplets in aqueous phase inside microfluidic chips and controlled evaporation of the organic solvent. Using this method, water-soluble nanomaterials can be easily encapsulated into biodegradable Poly(D,L-lactic-co-glycolic acid) (PLGA) to form functional microspheres. By controlling the flow rate of microemulsion phase, PLGA polymeric microspheres with narrow size distribution and diameters in the range of ∼50-100 μm were obtained.

A video-based speed estimation technique for localizing the wireless capsule endoscope inside gastrointestinal tract.

Wireless Capsule Endoscopy (WCE) is progressively emerging as one of the most popular non-invasive imaging tools for gastrointestinal (GI) tract inspection. As a critical component of capsule endoscopic examination, physicians need to know the precise position of the endoscopic capsule in order to identify the position of intestinal disease. For the WCE, the position of the capsule is defined as the linear distance it is away from certain fixed anatomical landmarks.

Measurement of motion detection of wireless capsule endoscope inside large intestine.

Wireless Capsule Endoscope (WCE) provides a noninvasive way to inspect the entire Gastrointestinal (GI) tract, including large intestine, where intestinal diseases most likely occur. As a critical component of capsule endoscopic examination, physicians need to know the precise position of the endoscopic capsule in order to identify the position of detected intestinal diseases. Knowing how the capsule moves inside the large intestine would greatly complement the existing wireless localization systems by providing the motion information.

Separation and purification of both tea seed polysaccharide and saponin from camellia cake extract using macroporous resin.

A novel method to separate and purify tea seed polysaccharide and tea seed saponin from camellia cake extract by macroporous resin was developed. Among four kinds of resins (AB-8, NKA-9, XDA-6, and D4020) tested, AB-8 macroporous resin possessed optimal separating capacity for the two substances and thus was selected for the separation, in which deionized water was used to elute tea seed polysaccharide, 0.25% NaOH solution to remove the undesired pigments, and 90% ethanol to elute tea seed saponin.

Separable bilayer microfiltration device for viable label-free enrichment of circulating tumour cells.

The analysis of circulating tumour cells (CTCs) in cancer patients could provide important information for therapeutic management. Enrichment of viable CTCs could permit performance of functional analyses on CTCs to broaden understanding of metastatic disease. However, this has not been widely accomplished.

Preparation of magnetic graphene composites with hierarchical structure for selective capture of phosphopeptides.

A novel graphene composite affinity material consisting of graphene scaffold, FeO nanoparticles for actuation and fully covered porous titania nanostructures as affinity coating has been designed and constructed. The obtained magnetic graphene composites have a saturation magnetization (Ms) value of 7.3 emu g, a BET specific surface area of 111.