Five previously undescribed citrinin derivatives from the endophytic fungus Penicillium citrinum GZWMJZ-836.

Penicillium citrinum GZWMJZ-836 is an endophytic fungus from Drynaria roosii Nakaike. Five previously undescribed citrinin derivatives (1-5) and six intermediates related to their biosynthesis (6-11) were obtained from the extract of this strain's solid fermentation using multiple column chromatography separations, including high-performance liquid chromatography. The structures of these compounds were determined through comprehensive spectroscopic analyses, primarily using NMR and HRESIMS data.

Indole Diterpene Derivatives from the GZWMJZ-288, an Endophytic Fungus from .

A new indole diterpene, 26-dihydroxyaflavininyl acetate (), along with five known analogs (-) were isolated from the liquid fermentation of GZWMJZ-288, an endophyte from . The structures of these compounds were identified through NMR, MS, chemical reaction, and X-ray diffraction experiments. Enzyme inhibition activity screening found that compounds , , and have a good binding affinity with NPC1L1, among which compound exhibited a stronger binding ability than ezetimibe at a concentration of 10 µM.

Two New 4-Hydroxy-2-pyridone Alkaloids with Antimicrobial and Cytotoxic Activities from sp. GZWMJZ-606 Endophytic with Thunb.

Two new 4-hydroxy-2-pyridone alkaloids furanpydone A and B ( and ), along with two known compounds N-hydroxyapiosporamide () and apiosporamide () were isolated from the endophytic fungus sp. GZWMJZ-606 in Thunb. Furanpydone A and B had unusual 5-(7-oxabicyclo[2.

The role of hexose transporter-like sensor hxs1 and transcription activator involved in carbohydrate sensing azf1 in xylose and glucose fermentation in the thermotolerant yeast Ogataea polymorpha.

Background: Fuel ethanol from lignocellulose could be important source of renewable energy. However, to make the process feasible, more efficient microbial fermentation of pentose sugars, mainly xylose, should be achieved. The native xylose-fermenting thermotolerant yeast Ogataea polymorpha is a promising organism for further development.

Sulfur-Containing Phenolic Compounds from the Cave Soil-Derived GZWMJZ-152.

Six new sulfur-containing phenolic compounds (-) and their putative metabolic precursors (-) were isolated from the cave soil-derived fungus GZWMJZ-152. Compound represents an unusual benzophenone-diketopiperazine hybrid via a thioether linker, while compound contains a naturally rare sulfoxide group. Both compounds and were initially isolated as racemic mixtures and then purified as the enantiomerically pure (+)-, (-)-, (+)-, and (-)-, respectively.

Development of new dominant selectable markers for the nonconventional yeasts Ogataea polymorpha and Candida famata.

Nonconventional yeast Candida famata and Ogataea polymorpha are interesting organisms for basic and applied studies. O. polymorpha is methylotrophic thermotolerant yeast capable of xylose alcoholic fermentation whereas C.

Cytotoxic Indolyl Diketopiperazines from the sp. GZWMJZ-258, Endophytic with the Medicinal and Edible Plant .

Two new indolyl diketopiperazines, gartryprostatins A and B ( and ), with an unusual 2,3-furan-fused pyrano[2,3-]pyrrolo[1″,2″:4',5']pyrazino[1',2':1,5]pyrrolo[2,3-]indole nucleus, along with a new naturally occurring compound (gartryprostatin C, ) were isolated from the solid culture of sp. GZWMJZ-258, an endophyte from (Guttiferae). The structures of compounds - were determined by nuclear magnetic resonance, mass spectrometry, Marfey's analysis of amino acids, and chemical calculation.

Semisynthetic Derivatives of Fradcarbazole A and Their Cytotoxicity against Acute Myeloid Leukemia Cell Lines.

Fourteen derivatives of the marine-derived fradcarbazole A were synthesized from staurosporine. Their structures were identified by NMR and high-resolution electrospray ionization mass spectrometry (HRESIMS). The derivatives were screened in vitro for antiproliferative activity against three human leukemic cell lines (MV4-11, HL-60, K562).

Cytotoxic phenolic constituents from Hypericum japonicum.

Nine undescribed compounds, including five xanthone derivatives, two flavonoids, one 2-pyrone derivative, and one undescribed naturally occurring compound, along with 30 known phenolic compounds, were isolated from Hypericum japonicum. In addition, hyperjaponols A and B were identified as racemates. The structures and absolute configurations of the undescribed compounds were determined by comprehensive MS, NMR spectroscopy, and electronic circular dichroism (ECD) calculations.

[Screening and antibacterial function of Bacillus amyloliquefaciens X030].

Objective: We isolated 339 bacillus strains from 72 soil samples all over the country, then purified their antimicrobial compounds and studied the antibacterial activity, to enrich bacillus resources and explore their second metabolites.

Methods: A bacillus strain with strong antibacterial activity was selected by dilution plate and water bath heating from a soil sample from a peanut plantation in Henan Province; this strain was identified according to morphological observation, physiological and biochemical characteristics, and consequences of 16S rRNA homologous analysis. Antibacterial compound from the identified strain, Bacillus amyloliquefaciens X030, was separated and purified by acetone precipitation, Sephadex chromatography, C18 reverse phase column chromatography.

Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022.

Background: Bacillus thuringiensis X022, a novel strain isolated from soil in China, produces diamond-shaped parasporal crystals. Specific mineral nutrients, such as Mg, Cu, and Mn, influence insecticidal crystal proteins (ICP) expression and the effects of these elements vary significantly. However, the molecular mechanisms of the effects caused by mineral elements have yet to be reported.

Anticancer Activity of Saponins from Allium chinense against the B16 Melanoma and 4T1 Breast Carcinoma Cell.

The cytotoxic substance of A. chinense saponins (ACSs) was isolated using ethanol extraction and purified with the D101 macroporous adsorption resin approach. We investigated the anticancer activity of ACSs in the B16 melanoma and 4T1 breast carcinoma cell lines.

Comparative analysis of genomics and proteomics in Bacillus thuringiensis 4.0718.

Bacillus thuringiensis is a widely used biopesticide that produced various insecticidal active substances during its life cycle. Separation and purification of numerous insecticide active substances have been difficult because of the relatively short half-life of such substances. On the other hand, substances can be synthetized at different times during development, so samples at different stages have to be studied, further complicating the analysis.

Purification and cloning of lectin that induce cell apoptosis from Allium chinense.

A 8.7 kDa lectin with high agglutin activity was isolated by affinity chromatography and cloned from Allium chinense in this study. For the MTT assay, approximately 60 µg/ml A.

Detection of toxin proteins from Bacillus thuringiensis strain 4.0718 by strategy of 2D-LC-MS/MS.

Bacillus thuringiensis is a kind of insecticidal microorganism which can produce a variety of toxin proteins, it is particularly important to find an effective strategy to identify novel toxin proteins rapidly and comprehensively with the discovery of the wild-type strains. Multi-dimensional high-performance liquid chromatography combined with mass spectrometry has become one of the main methods to detect and identify toxin proteins and proteome of B. thuringiensis.

MAP65-1a positively regulates H2O2 amplification and enhances brassinosteroid-induced antioxidant defence in maize.

Brassinosteroid (BR)-induced antioxidant defence has been shown to enhance stress tolerance. In this study, the role of the maize 65 kDa microtubule-associated protein (MAP65), ZmMAP65-1a, in BR-induced antioxidant defence was investigated. Treatment with BR increased the expression of ZmMAP65-1a in maize (Zea mays) leaves and mesophyll protoplasts.