NEK8 promotes the progression of gastric cancer by reprogramming asparagine metabolism.

Several members of the NIMA-related kinase (NEK) family have been implicated in tumor progression; however, the role and underlying mechanisms of NEK8 in gastric cancer (GC) remain unclear. This study revealed a significant upregulation of NEK8 in GC, identifying it as an independent prognostic marker in patients with GC. Consistent with these findings, NEK8 silencing substantially impeded GC aggressiveness both in vitro and in vivo, while its overexpression produced the opposite effect.

Local structural-functional coupling with counterfactual explanations for epilepsy prediction.

The structural-functional brain connections coupling (SC-FC coupling) describes the relationship between white matter structural connections (SC) and the corresponding functional activation or functional connections (FC). It has been widely used to identify brain disorders. However, the existing research on SC-FC coupling focuses on global and regional scales, and few studies have investigated the impact of brain disorders on this relationship from the perspective of multi-brain region cooperation (i.

Research hotspots and trends of neuroimaging in social anxiety: a CiteSpace bibliometric analysis based on Web of Science and Scopus database.

Background: This study focused on the research hotspots and development trends of the neuroimaging of social anxiety (SA) in the past 25 years.

Methods: We selected 1,305 studies on SA neuroimaging from the Web of Science and Scopus from January 1998 to December 2023. CiteSpace was used to analyze the number of published articles visually, cited references, cooperation among authors and institutions, co-occurrence of keywords, clustering of keywords, burst of keywords, and time zone of co-occurring keywords.

Discovery of SET domain-binding primary alkylamine-tethered degraders for the simultaneous degradation of NSD2-long and RE-IIBP isoforms.

Nuclear receptor binding SET domain protein 2 (NSD2) is involved in various pathologic processes and is considered as an important target for cancer therapy. Due to alternative splicing, NSD2 has 3 isoforms: long, short and RE-IIBP. Although previous studies reported the degradation of PWWP1 domain-containing NSD2-long and short isoforms through PWWP1-binding molecules, the degradation of RE-IIBP which does not contain PWWP1 has been neglected to date.

Combined Physiological and Transcriptomic Analysis Reveals Key Regulatory Networks and Potential Hub Genes Controlling Chilling Tolerance During Soybean Germination.

Chilling is an important limiting factor for seed germination of soybean ( [L.] Merr.).

Riemannian manifold-based disentangled representation learning for multi-site functional connectivity analysis.

Functional connectivity (FC), derived from resting-state functional magnetic resonance imaging (rs-fMRI), has been widely used to characterize brain abnormalities in disorders. FC is usually defined as a correlation matrix that is a symmetric positive definite (SPD) matrix lying on the Riemannian manifold. Recently, a number of learning-based methods have been proposed for FC analysis, while the geometric properties of Riemannian manifold have not yet been fully explored in previous studies.

Microfluidic SERS biosensor based on Au-semicoated photonic crystals for melanoma diagnosis.

Surface-enhanced Raman scattering (SERS) shows great promise for early diagnosis due to its high specificity and rapid detection capabilities. However, its application is often hindered by substrate instability and insufficient interaction between the substrate and incident light. To address these challenges, a photonic-plasmonic strategy is often employed to enhance sensing performance but it is generally limited by the low efficiency of plasmonic metal and optical cavity resonances.

CT-based delta-radiomics for predicting pathological response to neoadjuvant immunochemotherapy in esophageal squamous cell carcinoma: a multicenter study.

Background: The study aimed to investigate the predictive value of delta-radiomics derived from computed tomography (CT) for pathological complete response (pCR) to neoadjuvant immunochemotherapy (NICT) among patients with esophageal squamous cell carcinoma (ESCC), helping clinicians determine whether to modify the neoadjuvant treatment strategy, proceed to surgery, or forgo surgery altogether.

Methods: A total of 140 ESCC patients from two institutions (Database 1 = 93; Database 2 = 47) who underwent NICT and surgery were retrospectively included in the study. The training set consisted of patients from Database 1, while the testing set included patients from Database 2.

Accuracy of Percutaneous Pedicle Screw Placement Using Modified Single Anterior-Posterior Fluoroscopy with the C-Arm for the Treatment of Traumatic Thoracic and Lumbar Fractures.

Objective: We aimed to assess percutaneous pedicle screw (PPS) placement accuracy, fluoroscopy shot number, and operation time using a modified single anterior-posterior (AP) fluoroscopy with a C-arm in treating traumatic thoracic and lumbar vertebral fractures.

Methods: In total, 164 patients with single-level traumatic thoracic or lumbar fracture who underwent 6 PPS placements between April 2018 and September 2020 were divided into 2 groups. Conventional AP combined lateral fluoroscopy was used in Group A, whereas modified single AP fluoroscopy was used in Group B.

Discovery of CLPP-1071 as an Exceptionally Potent and Orally Efficacious Human ClpP Activator with Strong In Vivo Antitumor Activity.

caseinolytic protease P (ClpP) is essential for maintaining mitochondrial proteome homeostasis, and its activation is increasingly recognized as a promising cancer therapy strategy. Herein, based on structure-guided drug design, we discovered a series of potent ClpP activators by introducing a methyl group to the imipridone scaffold of the ClpP activator in Phase III clinical trials. Through structural optimization of the lead compound, the most optimal compound, , exhibited exceptionally potent ClpP agonistic activity (EC = 23.

Superior Photostability of the Unnatural Base 6-Amino-5-nitropyridin-2-ol: A Case Study Using Ultrafast Broadband Fluorescence, Transient Absorption, and Theoretical Computation.

6-Amino-5-nitropyridin-2-ol (Z), a nitroaromatic compound and a base for Hachimoji nucleic acids, holds significant potential in expanding the genetic alphabet, as well as in synthetic biology and biotechnology. Despite its promising applications, the spectral characterization and photoinduced properties of Z have remained largely unexplored until now. This study presents a comprehensive investigation into its excited state dynamics in various solvents, utilizing state-of-the-art ultrafast broadband time-resolved fluorescence and transient absorption spectroscopy, complemented by computational methods.

Research hot topics and frontiers in social anxiety over the past decade: a CiteSpace bibliometric analysis based on Web of Science database from 2013 to 2023.

Objective: To understand the current study of social anxiety (SA) over the past decade, and to analyze the research hot topics and frontiers in this field.

Methods: CiteSpace 6.2.

Plant kinetochore complex: composition, function, and regulation.

The kinetochore complex, an important protein assembly situated on the centromere, plays a pivotal role in chromosome segregation during cell division. Like in animals and fungi, the plant kinetochore complex is important for maintaining chromosome stability, regulating microtubule attachment, executing error correction mechanisms, and participating in signaling pathways to ensure accurate chromosome segregation. This review summarizes the composition, function, and regulation of the plant kinetochore complex, emphasizing the interactions of kinetochore proteins with centromeric DNAs (cenDNAs) and RNAs (cenRNAs).

Review of Laser Powder Bed Fusion's Microstructure and Mechanical Characteristics for Al-Ce Alloys.

As a new alloy manufacturing method that can break through the limitations of molds to manufacture fine parts, laser powder bed fusion has recently become a common process for producing aluminum alloys. In the fields of aerospace or automotive, aluminum alloys with both good printability and good mechanical performance in high-temperature conditions are greatly demanded, and the Al-Ce alloy is one of the alloys with significant potential. Therefore, systematic research on the additive manufacturing of Al-Ce alloys is still being explored.

Global burden of atrial fibrillation attributable to high body mass index from 1990 to 2021: findings from the Global Burden of Disease Study 2021.

Objectives: To assess the global burden of atrial fibrillation (AF) attributable to high body mass index (BMI) from 1990 to 2021 and analyze its spatiotemporal distribution characteristics.

Study Design: An observational study based on GBD 2021 data.

Methods: Data on AF burden due to high BMI were obtained from the Global Burden of Disease Study (GBD) 2021.

Can similarity of autistic traits promote neural synchronization?

People with similar levels of autistic traits are reported to exhibit better interactions than those with larger differences in autistic traits. However, whether this "similarity effect" exists at the neural level remains unclear. To address this gap, the present study employed functional near-infrared spectroscopy (fNIRS) hyperscanning technology to assess inter-brain synchronization (IBS) during naturalistic conversations among dyads with three types of autistic trait combinations (20 high-high, 22 high-low, and 18 low-low dyads).

Development and validation of a CT based radiomics nomogram for preoperative prediction of ISUP/WHO grading in renal clear cell carcinoma.

Background: Nuclear grading of clear cell renal cell carcinoma (ccRCC) is crucial for its diagnosis and treatment.

Objective: To develop and validate a machine learning model for preoperative assessment of ccRCC nuclear grading using CT radiomics.

Materials And Methods: This retrospective study analyzed 146 ccRCC patients who underwent surgery between June 2016 and January 2022 at two hospitals (the Quzhou Affiliated Hospital of Wenzhou Medical University with 117 cases and the Affiliated Cancer Hospital of University of Chinese Academy of Sciences with 29 cases).

The value of a nomogram model based on CT imaging features in differentiating duodenal gastrointestinal stromal tumors from pancreatic head neuroendocrine tumors.

Objective: To construct a nomogram model based on multi-slice spiral CT imaging features to predict and differentiate between duodenal gastrointestinal stromal tumors (GISTs) and pancreatic head neuroendocrine tumors (NENs), providing imaging evidence for clinical treatment decisions.

Methods: A retrospective collection of clinical information, pathological results, and imaging data was conducted on 115 cases of duodenal GISTs and 76 cases of pancreatic head NENs confirmed by surgical pathology at Zhongshan Hospital Fudan University from November 2013 to November 2022. Comparative analysis was performed on the tumor's maximum diameter, shortest diameter, long diameter/short diameter ratio, tumor morphology, tumor border, central position of the lesion, lesion long-axis direction, the relationship between tumor and common bile duct (CBD), duodenal side ulceration of the lesion, calcification, cystic and solid proportion within the tumor, thickened feeding arteries, tumor neovascularization, distant metastasis, and CT values during plain and enhanced scans in arterial and venous phases.

Discovery of KW0113 as a First and Effective PROTAC Degrader of DNMT1 Protein.

DNA methyltransferase 1 (DNMT1) is an attractive therapeutic target for acute myelocytic leukemia (AML) and other malignancies. It has been reported that the genetic depletion of DNMT1 inhibited AML cell proliferation through reversing DNA methylation abnormalities. However, no DNMT1-targeted PROTAC degraders have been reported yet.