Accurate and affordable detection of rifampicin and isoniazid resistance in Tuberculosis sputum specimens by multiplex PCR-multiple probes melting analysis.

Background: Escalating cases of multidrug-resistant tuberculosis (MDR-TB) pose a major challenge to global TB control efforts, necessitating innovative diagnostics to empower decentralized detection of gene mutations associated with resistance to rifampicin (RIF) and isoniazid (INH) in Mycobacterium tuberculosis (M. tuberculosis) in resource-constrained settings.

Methods: Combining multiplex fluorescent PCR and Multiple Probes Melting Analysis, we identified mutations in the rpoB, katG, ahpC and inhA genes from sputum specimens.

Down-expression of circulating micro ribonucleic acid (miRNA)-148/152 family in plasma samples of non-small cell lung cancer patients.

Aims: Circulating extracellular micro ribonucleic acids (miRNAs) are considered as potential biomarkers for malignancy detection and diagnosis. The aim of this study was to determine whether circulating miRNA-148/152 family (miR-148a, miR-148b, and miR-152) expression in plasma could be used as potential biomarkers for non-small cell lung cancer (NSCLC) patients and healthy individuals.

Subjects And Methods: The levels of miRNA-148/152 family were detected by TaqMan quantitative polymerase chain reaction (qPCR) assay in plasma of 20 NSCLC patients and 10 healthy individuals.

Stiffness of various pin configurations for pediatric supracondylar humeral fracture: a systematic review on biomechanical studies.

To compare the biomechanical stability of various pin configurations for pediatric supracondylar humeral fractures under varus, internal rotation, and extension conditions. After electronic retrieval, 11 biomechanical studies were included. Stiffness values of pin configurations under different loading conditions were extracted and pooled.

Novel species including Mycobacterium fukienense sp. is found from tuberculosis patients in Fujian Province, China, using phylogenetic analysis of Mycobacterium chelonae/abscessus complex.

Objective: To identify the novel species 'Mycobacterium fukienense' sp. nov of Mycobacterium chelonae/abscessus complex from tuberculosis patients in Fujian Province, China.

Methods: Five of 27 clinical Mycobacterium isolates (Cls) were previously identified as M.

Multicenter research on the BACTEC MGIT 960 system for the second-line drugs susceptibility testing of Mycobacterium tuberculosis in China.

The reliability of the BACTEC MGIT 960 system for the second-line drugs (capreomycin [CPM], kanamycin [KAN], ofloxacin [OFX] and ethionamide [ETH]) susceptibility testing (DST) of Mycobacterium tuberculosis (M. tuberculosis) was compared to that of traditional Lowenstein-Jensen (L-J) proportion method (PM) among four different sites in China. After resolution of discrepant results by retesting the strains using both methods in the National Reference Laboratory of tuberculosis, the overall concordance values between the 2 systems were 99.

[Quantitative evaluation of soil hyperspectra denoising with different filters].

The noise distribution of soil hyperspectra measured by ASD FieldSpec Pro FR was described, and then the quantitative evaluation of spectral denoising with six filters was compared. From the interpretation of soil hyperspectra, the continuum removed, first-order differential and high frequency curves, the UV/VNIR (350-1 050 nm) exhibit hardly noise except the coverage of 40 nm in the beginning 350 nm. However, the SWIR (1 000-2 500 nm) shows different noise distribution.

[Study on the characteristics of mutation on Mycobacterium tuberculosis rifampicin-resistance gene].

Objective: To elucidate the characters of rpoB mutation in rifampin-resistant clinical isolates of Mycobacterium tuberculosis.

Methods: 286 bp DNA fragment of rpoB gene including 81 bp code region (rifampin resistance deteremination region, RRDR) was analyzed by PCR-single-strand conformation polymorphism(SSCP). The 286 bp DNA fragment of each strain which had been proved to have mutation by PCR-SSCP was then sequenced.

Detection of coastal saline land uses with multi-temporal landsat images in Shangyu City, china.

Many coastal regions in China are confronted with pressing problems of scarce land resources and heavy population. Over the past 30 years, considerable parts of coastal tidelands have been enclosed and reclaimed for agricultural land uses. To assess, plan, and implement large-scale reclamation programs, up-to-date and reliable information concerning the nature, areal extent, and physical and chemical characteristics of coastal saline lands is essential.