Application of atomic force microscopy in blood research.

Aim: To find suitable solutions having lesser granules and keeping erythrocytes in normal shapes under atomic force microscopy (AFM).

Methods: Eight kinds of solutions, 1% formaldehyde, PBS buffer (pH7.2), citrate buffer (pH 6.

[Influence of dendritic cell infiltration on prognosis and biologic characteristics of progressing gastric cancer].

Objective: To study the relation between dendritic cell (DC) infiltration and clinicopathologic parameters, biologic characteristics and prognosis of progressing gastric cancer.

Methods: The development of apoptotic cell death (apoptotic index, AI) in 61 progressing gastric carcinoma tissues was analyzed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end labeling (TUNEL) method. The PCNA labeling index (PCNA-LI), density of dendritic cells in the tumor were detected by immunohistochemical method by the LSAB kit using antibody against S-100 protein and PC-10.