Publications by authors named "Ming-hui Li"
[Expansion ex vivo of human bone marrow mesenchymal stem cells and cord blood CD34+ cells].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
April 2005
The aim was to study the roles that the bone marrow mesenchymal stem cells (MSC) and cytokines play in cord blood CD34(+) cell expansion ex vivo and the influence of culture ex vivo on expression of the adhesive molecule of CD44. CD34(+) cells sorted from cord blood cells had been cultured in each well of 24 well culture plates containing culture medium supplemented with mesenchymal stem cells layer or/and cytokines for a week, and then all kinds of indexes of different groups were compared. The results showed that as for cord blood cell expansion, there was no significant difference between the groups with cytokines SDF-1alpha + SCF + TPO + FL and SCF + TPO + FL no matter if MSC layer existed or not.
View Article and Find Full Text PDF[Difference and significance of T-lymphocyte subsets in differential diagnosis between severe acute respiratory syndrome and common atypical pneumonia].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
June 2004
Background: To clarify the difference and significance of T-lymphocyte subsets in differential diagnosis between severe acute respiratory syndrome SARS) and common atypical pneumonia.
Methods: Totally 100 patients hospitalized in Beijing Ditan Hospital since March to June 2003 with clinical diagnosis of SARS were involved in this study. These patients courses of disease were over 3 weeks.
High-voltage-activated Ca2+ channels are essential for diverse biological processes. They are composed of four or five subunits, including alpha1, alpha2-delta, beta and gamma (ref. 1).
View Article and Find Full Text PDFTo understand the processes involved in the catalytic mechanism of pyridoxal kinase (PLK),1 we determined the crystal structures of PLK.AMP-PCP-pyridoxamine, PLK.ADP.
View Article and Find Full Text PDFThe three-dimensional structures of brain pyridoxal kinase and its complex with the nucleotide ATP have been elucidated in the dimeric form at 2.1 and 2.6 A, respectively.
View Article and Find Full Text PDFCrystallization and preliminary crystallographic studies of pyridoxal kinase from sheep brain.
Acta Crystallogr D Biol Crystallogr
September 2002
Article Synopsis
- Pyridoxal kinase is an important enzyme that converts vitamin B(6) to its active form, pyridoxal-5'-phosphate, which is essential for amino acid metabolism and neurotransmitter synthesis.
- The enzyme was successfully crystallized from sheep brain using the hanging-drop vapor-diffusion technique and sodium citrate as a precipitant.
- The resulting crystals are of high quality, belonging to a specific space group and capable of diffracting at a resolution of 2.1 A, and were further treated to prepare heavy-atom derivatives for analysis.