[Study on relationship between hepatitis B virus DNA load and genotype with large envelope protein].

Objective: To explore the relation between hepatitis B virus DNA load and genotype with the level of large envelope protein.

Methods: Serum HBV DNA was quantitively detected by using real time polymerase chain reaction (RT-PCR). The LHBs were detected by using enzyme linked immuno sorbent assay (ELISA) and HBV markers were detected by time differentiate immunofluorescence assay in 140 serum samples collected from chronic hepatitis B patients.