Differential impact of wildfire and rice straw ash on the skin microbiota of Rana dybowskii adults and tadpoles.

Global warming has intensified severe weather conditions and increased the frequency of wildfires, posing significant threats to ecosystems. Moreover, rice straw ash, a byproduct of human agricultural activities, represents an environmental stressor that can further impact these vulnerable ecosystems. These changes particularly impact sensitive species and microorganisms, yet limited research has explored the effects of wildfire ash and agricultural byproducts, such as rice straw ash, on amphibians.

Synergistic toxicity of cadmium and triadimefon on the microbiota and health of Rana dybowskii tadpoles.

The skin and gut microbiota are crucial to amphibians. Triadimefon (TF), a widely used triazole fungicide, controls crop diseases and regulates growth, with uncertain effects on amphibian microbiota. Contamination, typically involving mixed chemicals at low concentrations, including cadmium (Cd) and TF, may detrimentally affect amphibian growth, survival, and microbiota health in both the skin and gut, but few research has examined these consequences.

Impact of wildfire ash on skin and gut microbiomes and survival of Rana dybowskii.

Climate change and human activities escalate the frequency and intensity of wildfires, threatening amphibian habitats and survival; yet, research on these impacts remains limited. Wildfire ash alters water quality, introduces contaminants, and may disrupt microbial communities, impacting gut and skin microbiota; however, the effects on gut and skin microbiota remain unclear. Rana dybowskii were exposed to five concentrations (0 g L, 1.

Rice straw ash and amphibian health: A deep dive into microbiota changes and potential ecological consequences.

Rice straw is burned as a result of agricultural practices and technical limitations, generating significant volumes of ash that might have environmental and ecological consequences; however, the effects on organisms have not been researched. Amphibians depend on their gut and skin microbiomes. Ash exposure may cause inflammation and changes in microbial diversity and function in frogs' skin and gut microbiota due to its chemical composition and physical presence, but the implications remain unclear.

Characteristics and a comparison of the gut microbiota in two frog species at the beginning and end of hibernation.

Season has been suggested to contribute to variation in the gut microbiota of animals. The complicated relationships between amphibians and their gut microbiota and how they change throughout the year require more research. Short-term and long-term hypothermic fasting of amphibians may affect gut microbiota differently; however, these changes have not been explored.

[Microsurgical repair of skin and soft tissue defects on head, face, and neck].

Objective: To study the microsurgical method of repairing skin and soft tissue defects on head, face, and neck.

Methods: Thirty-one patients with skin and soft tissue defects on the head, face, or neck were hospitalized from July 2007 to May 2010, including 10 cases of scalp defects, 4 cases of skin and soft tissue defects on face, and 17 cases of skin and soft tissue defects on neck. Among them, the cause in 20 cases was trauma, and in 11 cases they were secondary to release of cicatricial contraction.

[Repair of devastating wounds with free composite tissue flap].

Objective: To study the safety and effects of free composite tissue flaps in repairing devastating wounds in early stage.

Methods: One hundred and twenty-three patients with 128 devastating wounds hospitalized in our burns center from 2005 to 2009 were repaired with free flaps or composite tissue flaps. Flap types used included 58 latissimus dorsi muscular flaps, 32 anterolateral thigh flaps, 21 circumflex scapular flaps, 6 dorsalis pedis composite flaps, 3 big toe nail skin flaps, 3 forearm flaps, and 1 lateral thoracic flap.

[Surgical therapy for massive deep skin and soft tissue injuries].

Objective: To explore the methods of repair of massive deep skin and soft tissue injuries.

Methods: Fifty-six patients with deep skin and soft tissue injuries were hospitalized from July 2006 to January 2008. Among them, 23 cases were caused by burn, 17 cases by electric injury, 7 cases by hot crush injury, 6 cases by avulsion injury, and 3 cases due to other reasons (including traffic accident, crush injury, soft tissue infection respectively).

[Functional reconstruction of multiple severe deformities after extensive deep burn].

To explore new measures for functional reconstruction of multiple severe deformities as a result of extensive deep burn (total burn surface area > or = 90% TBSA, including deep burn > or = 70%TBSA) in late stage. Twelve severe burn patients with above-mentioned deformities were hospitalized in our ward during 1960--2005, the scars resulted from burns were distributed from head to foot with 173 deformities, including 27 scar ulcers. All patients lacked of self-care ability, among them some could not stand.

[Evaluation and surgical treatment for chronic wound of leg and foot].

Objective: To investigate the optimal operation method for the management of various chronic wounds in legs and feet.

Methods: Fifty-one chronic wounds were evaluated according to infection, inflammatory response, and distribution in different areas of the leg and foot. Preoperative treatment was given accordingly, then transposition of skin flap, skin grafting, or amputation was performed.

[Experimental study and clinical application of composite skin grafting].

Objective: To explore an ideal skin substitute with its appearance and texture similar to normal skin, to repair wounds with full-thickness skin defect.

Methods: Composite skin (CS) in question was composed of allo/xenogeneic acellular dermal matrix (ADM) and razor thin autoskin. One step skin grafting was employed in the experimental study and clinical trial.

Expression of human beta-defensin 3 in COS-7 cell.

Objective: To establish a cell line for stable expression of human beta-defensin 3 (hBD3).

Methods: Full length cDNA of hBD3 was isolated from previously constructed pGEM-hBD3 and then inserted into pcDNA3. The recombinant vector identified carrying hBD3 with right direction was introduced into COS-7 cells by Lipofectamine.

[Establishment of rat model of scalding with high pressure steam].

Objective: To establish a rat model of scalding with controllable depth and area by high pressure steam.

Methods: High pressure steam apparatus consisting of an autoclave and a self-made scalding frame was employed in the study. The rats were inflicted with scalding with 0.

[Repair of contracture deformities of the patients with extremely limited donor skin at late postburn stage].

Objective: To explore new methods to repair postburn contracture deformities in patients with extremely limited donor skin.

Methods: Five severely burned patients with extremely limited donor skin but severe deformities were enrolled in the study. The mature and the pliable scarred skin was utilized as the donor site for reconstruction of the postburn deformities.

[An experimental study on the change in neuropeptide substance P in the burn wound skin in scalded rats].

Objective: To investigate the change in neuropeptide substance P (SP) in the burn wound skin in scalded rats.

Methods: The SP distribution density in the rat skin samples from the burn wound, peri-wound area and remote area of scalded rats were determined by immunohistochemistry technique. The mRNA expression of SP was observed by in situ hybridization.

[Long-term in vitro culture of the fibroblasts from the deep partial thickness burn wound in burn patients].

Objective: To establish a long-term in vitro culture of the fibroblasts obtained from burn wounds.

Methods: Skin samples were harvested from normal volunteers and the deep partial thickness burn wound in burn patients on the 5th, 10th, 21st, 28th and 35th postburn days (PBDs). The non-dermal tissue was removed from the samples and primed by chlorhexidine solution in concentration of 2.