Combined Transcriptome and Metabolome Analysis of Laur. Peel Treated With UV-C Reveals the Involvement of Key Metabolic Pathways.

An increasing attention is being given to treat fruits with ultraviolet C (UV-C) irradiation to extend shelf-life, senescence, and protection from different diseases during storage. However, the detailed understanding of the pathways and key changes in gene expression and metabolite accumulation related to UV-C treatments are yet to be explored. This study is a first attempt to understand such changes in banana peel irradiated with UV-C.

[Study on expression of Treg cells in attenuated Schistosoma japonicum cercariae immunized BALB/c mice].

Objective: To study the expression differences of CD4+CD25+Foxp3+Treg cells between the attenuated cercariae immunized mice and the normal infected mice and discuss the immune protection mechanisms of the mice immunized with attenuated cercariae.

Methods: Forty female BALB/c mice were divided into 2 groups, group A, the attenuated cercariae immunized group (16 mices) and the group B, the normal cercariae infected group (16 mices), and the last 8 ones served as the blank control. The spleen cells and the ratios of PBMC's CD4+CD25+Foxp3+/CD4+CD25+T cells were compared between the attenuated cercariae immunized mice and normal mice injected by FCM and the Foxp3 expression levels in spleens and livers were assayed by IHC.

[Analysis of karyotype and preparation of C-band and G-band in Schistosoma japonicum].

Chromosomes of Schistosoma japonicum were prepared by usual air drying method, C-band and G-band were made by modified BSG method and enzyme digestion method respectively. Results showed that the karyotype of S. japonicum was 4m + 6Sm + 4St + 2sex chromosome and the C-band formula was 2n = 5CIt + 4CI(+) + 3CI + 2CT +2CT.

[Alkaloid constituents of Corydalis adunca].

Objective: To study the alkaloid component of Corydalis adunca Maxim.

Methods: The constituents were isolated by chromatographic methods, their structures were elucidated by spectroscopic evidences.

Results: Three compounds were purified and their structures were identified, they were identified as dorydaline (I), dehydrocorydaline (II), dihydrosanguinarine (III).