Tea Polysaccharides Inhibit Colitis-Associated Colorectal Cancer via Interleukin-6/STAT3 Pathway.

The interleukin-6 (IL-6)/signal transducer and activator of transcription (STAT)-3 signaling pathway regulates proliferation and survival of intestinal epithelial cells and has profound impact on the tumorigenesis of colitis-associated cancer (CAC). Tea polysaccharides (TPS) are the major nutraceutical component isolated from tea-leaves and are known to possess antioxidant, anti-inflammatory, and antitumor bioactivities. Here, we investigated the antitumor activities of TPS on CAC using the azoxymethane/dextran sulfate sodium (AOM/DSS) mouse model and IL-6-induced colorectal cancer cell line (CT26) and determined whether TPS exerted its antitumor effects through the IL-6/STAT3 pathway.

Tea Polysaccharide Prevents Colitis-Associated Carcinogenesis in Mice by Inhibiting the Proliferation and Invasion of Tumor Cells.

The imbalance between cell proliferation and apoptosis can lead to tumor progression, causing oncogenic transformation, abnormal cell proliferation and cell apoptosis suppression. Tea polysaccharide (TPS) is the major bioactive component in green tea, it has showed antioxidant, antitumor and anti-inflammatory bioactivities. In this study, the chemoprophylaxis effects of TPS on colitis-associated colon carcinogenesis, especially the cell apoptosis activation and inhibition effects on cell proliferation and invasion were analyzed.

Carboxymethylation of polysaccharide from Cyclocarya paliurus and their characterization and antioxidant properties evaluation.

In this study, three chemically carboxymethyl polysaccharides (CM-CPs) were derived from Cyclocarya paliurus polysaccharides. The physicochemical properties and antioxidant activity in vitro of carboxymethyl derivatives were determined. The results of degree of substitution and FT-IR analysis showed the carboxymethylation of polysaccharides were successful.

Preparation, characterization and antioxidant activities of acetylated polysaccharides from Cyclocarya paliurus leaves.

In this study, polysaccharides extracted from Cyclocarya paliurus leaves were modified to obtain its three acetylated derivatives, Ac-CP1, Ac-CP2, and Ac-CP3. The physicochemical characteristics and antioxidant activities of acetylated derivatives were investigated. The results of chemical and FT-IR spectrum analysis showed differences between acetylated derivatives and native C.

Sulfated polysaccharides from Cyclocarya paliurus reduce H2O2-induced oxidative stress in RAW264.7 cells.

In this study, two sulfated polysaccharides (S-CP1-4 and S-CP1-8) from Cyclocarya paliurus were produced by chlorosulfonic acid-pyridine method. Hydrogen peroxide (H2O2) was used to develop an oxidative stress model in the mouse macrophage cell line RAW264.7.

Extraction, chemical composition and antioxidant activity of flavonoids from Cyclocarya paliurus (Batal.) Iljinskaja leaves.

Microwave-assisted extraction of flavonoids from Cyclocarya paliurus (Batal.) Iljinskaja leaves, its chemical composition and antioxidant activity were investigated in this study. The influences of parameters including temperature, extraction time, ratio of material to solvent and solvents on the yield of flavonoids were studied.

Separation of water-soluble polysaccharides from Cyclocarya paliurus by ultrafiltration process.

In this study, ultrafiltration membrane process was employed to separate polysaccharides from Cyclocarya paliurus (Batal.) Iljinskaja (C. paliurus) to simulate industrial production.

Analysis of monosaccharide composition of Cyclocarya paliurus polysaccharide with anion exchange chromatography.

A simple, rapid and sensitive analytical method for the determination of monosaccharide composition in Cyclocarya paliurus polysaccharide was developed and validated. This method was based on hydrolysis of the polysaccharides followed by high-performance anion-exchange chromatography analysis. The effects of sodium hydroxide concentration and column temperature on retention and separation of the monosaccharide were investigated with Carbo PAC™ PA10 analytical column.

Simultaneous analysis of 18 mineral elements in Cyclocarya paliurus polysaccharide by ICP-AES.

The contents of 18 kinds of mineral elements in Cyclocarya paliurus polysaccharide samples were determined by ICP-AES. The limits of detection (LOD) of the method for 18 elements were in the range of 0.01-3.

Purification, physicochemical characterisation and anticancer activity of a polysaccharide from Cyclocarya paliurus leaves.

A Cyclocarya paliurus (Batal.) Iljinskaja polysaccharide (CPP) was isolated and purified by hot water extraction, ethanol precipitation, deproteinisation and anion-exchange chromatography. Its physicochemical properties were characterised by gel permeation chromatography (GPC), gas chromatography-mass spectrometry (GC-MS), thermal gravimetric analysis (TGA), Fourier transform infrared spectrometry (FTIR), UV-visible spectrophotometry, dynamic light scattering (DLS) and viscometry analysis.

Ultrasonic-assisted extraction, antimicrobial and antioxidant activities of Cyclocarya paliurus (Batal.) Iljinskaja polysaccharides.

Recently, renewed interest has grown in the use of ultrasonic-assisted extraction as an alternative approach to the traditional extraction methods. In this study, this novel extraction method was used to isolate polysaccharides from Cyclocarya paliurus (Batal.) Iljinskaja, and response surface methodology (RSM) was employed to optimize the extraction conditions.

Optimisation of microwave-assisted extraction of polysaccharides from Cyclocarya paliurus (Batal.) Iljinskaja using response surface methodology.

Background: Cyclocarya paliurus (Batal.) Iljinskaja is a well-known edible and medicinal plant in China and is commonly called the 'sweet tea tree'. It has been reported to have a broad range of therapeutic and nutritional values in lowering blood sugar, blood lipids and blood pressure.

Simultaneous determination of organophosphorus pesticides in Chinese herbal medicines by microwave-assisted extraction coupled with dispersive-solid phase extraction and gas chromatography.

A reliable, rapid and nontoxic analytical method was proposed for the simultaneous determination of 16 organophosphorus pesticides in Chinese herbal medicines. The pesticides were extracted by ethanol and the experimental variables, such as temperature, extraction time and volume of ethanol, were optimized through orthogonal array experimental design. Cleanup of extracts was performed with dispersive-solid phase extraction using primary secondary amine as the sorbent.