Mining and Engineering the Di--glycosylation Pattern of UGT72B1 for the Highly Efficient -Glycosylation of Endogenous Quercetin.

Compared with mono--glycosylation, di--glycosylation endows the precursor with better performance. However, the mining and engineering of di--glycosylation patterns of glycosyltransferases are limited, hindering their synthetic applications. Here, an xenobiotic-transforming glycosyltransferase, UGT72B1, was found to catalyze the glycosylation of endogenous quercetin and its monoglycosides, generating di-glucosides.