[Study on genetic diversity of natural and cultivated Cistanche tubulosa].

Objective: To determine the genetic diversity of natural and cultivated Cistanche tubulosa.

Method: 123 individuals of six populations of C. tubulosa, including four natural populations and two cultivated ones, were analyzed by random amplified polymorphic DNA (RAPD) markers to determine the genetic variations among the populations.

[The determination of echinacoside and acteoside in herbs of Cistanche tubulosa].

Objective: To analyse the echinacoside and acteoside content of Cistanche tubulosa, collected from different hosts and different size of the cultivated, which is compared to the wilding by RP-HPLC method.

Method: An Agilent Eclipse XDB-C18(4.6 mm x 250 mm, 5 microm) column was used and a mixture of methanol-acetonitrile-1% acetic acid (15:10:75) was used as the mobile phase at a flow rate of 0.