Transcriptomics and iTRAQ-proteomics analyses provide novel insights into the defense mechanism of black shank disease in tobacco.

Black shank disease caused by is one of the most important diseases in tobacco worldwide and can result in a devastating loss in tobacco cultivation. Many efforts have been carried out to identify the chromosome segment from containing a resistance locus carrying a gene named ; however, the gene has not been cloned, and knowledge of the potential mechanism of the gene in the resistant lines is limited. To further characterize the resistance mechanism of the gene, we first used the resistant line "RBST" and the susceptible cultivar "Honghuadajinyuan" (HD) to obtain the near-isogenic line RBS89 containing the gene from RBST.

Genome-wide identification and expression analysis of NtbHLH gene family in tobacco () and the role of NtbHLH86 in drought adaptation.

The bHLH transcription factors play pivotal roles in plant growth and development, production of secondary metabolites and responses to various environmental stresses. Although the genes have been well studied in model plant species, a comprehensive investigation of the genes is required for tobacco with newly obtained high-quality genome. In the present study, a total of 309 genes were identified and can be divided into 23 subfamilies.