An immunonanogold resonance scattering spectral probe for rapid assay of human chorionic gonadotrophin.

Background: Several assays for human chorionic gonadotrophin (hCG) such as radioimmunoassay and fluorescence immunoassays were reported. So far, there was no report about the immunonanogold resonance scattering spectral (ING-RSS) assay based on the immunoreaction and resonance scattering (RS) effect.

Methods: Three ING-RSS probes for hCG were prepared, using nanogold in size of 8, 10, 13 nm to label rabbit hCG antiserum (anti-hCG).