Leptin activates the JAK/STAT pathway to promote angiogenesis in RF/6A cells .

Aim: To investigate the effect of leptin on the angiogenesis of RF/6A cells (monkey retinal choroidal endothelial cells) and test the cellular signaling in the mechanism.

Methods: RF/6A cells were cultured and randomly divided into four groups: normal control, with leptin at 50, 100, 200 ng/mL for cell counting kit-8 (CCK8). RF/6A cell proliferation and migration were examined by Transwell assays, while RF/6A cell tube formation by Matrigel assay.