Comparative Study on Different Remediation Strategies Applied in Petroleum-Contaminated Soils.

Due to the increasing pollution by petroleum hydrocarbons (PHs), it is an important task to develop eco-friendly and highly efficient methods for remediating petroleum-contaminated soils. In this study, bioremediation technology was applied to remediate PHs contaminated soils, and the bacterial community structure and physicochemical characteristics of the soil treated using different bioremediation regimens were analyzed. Compared with the control condition (S0), the PHs removal efficiency of biostimulation (S2) and bioaugmentation (S3) was increased significantly.

Effects of on Root Metabolites and Rhizosphere Soil Properties to Continuously-Cropped Soybean in the Potted-Experiments.

Continuous cropping in soybean is increasingly practiced in Heilongjiang Province, leading to substantial yield reductions and quality degradation. Arbuscular mycorrhizal fungi (AMF) are soil microorganisms that form mutualistic interactions with plant roots and can restore the plant rhizosphere microenvironment. In this study, two soybean lines (HN48 and HN66) were chosen as experimental materials, which were planted in different years of continuous cropping soybean soils and were inoculated or not with in potted-experiments.

[The effect of aluminum adjuvant and immunization schedule on immunogenicity of Sabin inactivated poliovirus vaccine].

Objective: To study the effect of aluminume adjuvant and immunization schedule on immunogenicity of Sabin inactivated poliovirus vaccine.

Methods: Four batches of Sabin IPV were produced by different concentrations of type 1, 2, and 3 poliovirus and administrated on three-dose schedule at 0, 1, 2 months and 0, 2, 4 months on rats. Serum samples were collected one month after each dose and neutralizing antibody titers against three types poliovirus were determined by micro-neutralization assay.

[Hydroacoustic assessment of fish resources in reservoirs with different fishery management].

By using Biosonics DT-X echosounder (208 kHz), a hydroacoustic investigation was conducted on the fish resources in three mesotrophic reservoirs (Daxi Reservoir, Shahe Reservoir, and Jinniushan Reservoir) with different fishery management in late autumn and early winter 2011, and a GIS model was constructed to assess the fish resource distribution in the reservoirs. No significant difference was observed in the average size of fish in the three reservoirs, but the distribution curves of fish target strength (TS) showed that the fish size distribution proportion was different, and had close correlation with fishery management. The fish density in Daxi Reservoir (averagely 0.

[Experimental study on the environmental fate of nitrogen in snail-macrophyte ecosystem for water purification].

A snail-macrophyte simulation system was built and isotope tracer technique was adopted to study the environmental fate of nitrogen in snail-macrophyte purification system, the results showed that: Vallisneria spiralis increased its wet weight by 580% and its number by 6.6 ramets, moreover, Vallisneria spiralis absorbed 1.07% 15N by the roots and 7.

[Immunogenicity of sabin inactivated poliovirus vaccine induced by diphtheria-tetanus-acellular pertussis and Sabin inactivated poliovirus combined vaccine].

Objective: In order to search the preparation process and optimazing dosage ratio of adsorbed diphtheria-tetanus-acellular pertussis and sabin inactivated poliovirus combined vaccine (DTaP-sIPV), the neutralizing antibody titers of IPV induced by different concentration of DTaP-sIPV were investigated on rats.

Methods: Two batches of DTaP-sLPV were produced using different concentration of sIPV and the quality control was carried. Together with sabin-IPV and DTaP-wIPV ( boostrix-polio, GSK, Belgium) as control group, the DTaP-sIPV were administrated on three-dose schedule at 0, 1, 2 month on rats.

[Preparation of vero cell-adapted influenza H5N1 virus strain by genetic reassortment].

Objective: To prepare Vero cell-adapted influenza H5N1 virus strain by Genetic Reassortment and produce influenza H5N1 vaccine using Vero cell as a substrate.

Methods: Embryonated specific pathogen-free (SPF) hen's eggs and Vero cells were co-infected with Vero cell-adapted influenza virus A/Yunnan/1/2005 Va(H3N2) and A/Anhui/1/2005 (H5N1) via reverse genetics. The reassortant was screened with goat antibody against strain A/Yunnan/1/2005 Va(H3N2) and identified for subtype by hemagglutination-inhibition (HI) assays and gene analysis of HA and NA.

Screening of a high growth influenza B virus strain in Vero cells.

Due to the insufficient supply of embryonated chicken eggs, the preparation of large quantities of inactivated influenza vaccines will require an alternative virus culture system after the emergence or reemergence of a pandemic influenza virus. The Vero cell is one of the ideal options since it was used for producing many kinds of human vaccines. However, most of the influenza viruses can not grow well in Vero cells.

[Research on an improved Lowry method to determine content of protein in Sabin IPV].

Objective: To establish a method for the content determination of protein in Sabin IPV.

Methods: Using lowry method combined with being precipitated by trichloroacetic acid to determine the content of protein in Sabin IPV. Changing different conditions to optimize the experiment to establish a improved lowry method.

[Effective inactivatian test of inactivated hepatitis A vaccine using integrated cell culture/strand-specific reverse transcriptase-polymerase chain reaction].

Objective: To establish an quick, sensitive and specific assay for effective inactivatian test of inactivated hepatitis A vaccine.

Methods: effective inactivatian test of inactivated hepatitis A vaccine were carried out using integrated cell culture/strand-specific RT-PCR (ICC/strand-specific RT-PCR) assay compared with traditional ELISA and nest RT-PCR assay.

Results: all the samples were infectious negative detecting by both ICC/ strand-specific RT-PCR and ELISA assay,while some samples appeared false positive detecting by nest RT-PCR.

[Effect of 2-phenoxyethanol on potency of Sabin inactivated poliomyelitis vaccine and its safety].

Objective: To investigate the effect of 2-phenoxyethanol on potency of Sabin inactivated poliomyelitis vaccine (IPV).

Methods: Sabin IPV samples containing 5 mg or 7 mg 2-phenoxyethanol each dosage respectively were placed separately at 4 degrees C, 37 degrees C for 2 days and 7 days. D-antigen contents were tested with ELISA method.

A novel process for production of hepatitis A virus in Vero cells grown on microcarriers in bioreactor.

Aim: To develop a novel process for production of HAV in Vero cells grown on microcarriers in a bioreactor.

Methods: Vero cells infected with HAV strain W were seeded at an initial density of 1 x 10(5) cells/mL into a 7-L bioreactor containing Cytodex-I microcarriers. During the stage of cell proliferation, the following conditions were applied: pH 7.

[Immunogenecity of combined hepatitis A and B vaccine].

Objective: To observe the immunogenicity of combined hepatitis A and B vaccine (HAB).

Methods: The combined HAB vaccine was prepared and different concentrations of HAB were administered on mice in week 0, 4 and 24, and then we tested the antibodies to both hepatitis A virus and B virus. After the first injection, we tested the hepatitis A antigen-induced and hepatitis B surface antigen-induced stimulation indices in spleen monocyte as well as changes of CD4+ and CD8+ cell numbers.