Genomic surveillance of emerging SARS-CoV-2 Omicron variations in Tianjin Municipality, China 2022.

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has severely impacted public health. In 2022, the Omicron variant of SARS-CoV-2 rapidly became the dominant circulating variant in the local COVID-19 outbreaks in Tianjin Municipality, China. To gain a deeper understanding of the genetic variations of the Omicron variant in Tianjin, specimens from individuals who tested positive for SARS-CoV-2 between December 2021 and November 2022 were used for virus whole genome sequencing and phylogenetic analysis.

cGAMP promotes inner blood-retinal barrier breakdown through P2RX7-mediated transportation into microglia.

Background: Impairment of the inner blood-retinal barrier (iBRB) leads to various blinding diseases including diabetic retinopathy (DR). The cGAS-STING pathway has emerged as a driving force of cardiovascular destruction, but its impact on the neurovascular system is unclear. Here, we show that cGAMP, the endogenous STING agonist, causes iBRB breakdown and retinal degeneration thorough P2RX7-mediated transport into microglia.

Molecular interaction study of flavonols with human serum albumin by theoretical simulations.

The human serum albumin (HSA)-drug binding characteristic is directly related to the pharmacokinetic, pharmacodynamic profile and side effect properties of drug. Though much is known about the protein binding characteristics of flavonol, the influence of molecular structure of flavonol and dynamic behaviour of protein conformations have received little attention. Here, we studied the interactions of HSA with flavonols by using molecular docking, molecular dynamics simulations coupled with molecular mechanics- Poisson Boltzmann/generalized Born surface area (MM-PB/GBSA) method.

Bacterial Community Structures in Raw Pork from Supermarkets and Farmers' Markets Determined by High-Throughput Sequencing Analysis.

Pork contaminated with bacteria can shorten the shelf life and cause foodborne diseases. Bacterial community analysis of raw pork in sale process can help identify bacteria associated with food quality and safety. In this study, 52 pork samples were collected from various supermarkets and farmers' markets in Qingdao, China.

Priming agents combat copper stress in wheat (Triticum aestivum L.) under hydroponic conditions: Insights in impacts on morpho-physio-biochemical traits and health risk assessment.

In recent years, the use of priming agents, such as silicon, melatonin, salicylic acid, glycine betaine, and ascorbic acid has gained significant attention for their role in mitigating abiotic stresses across various plant species. While previous research has been conducted on the individual impact of silicon, melatonin, salicylic acid, glycine betaine, and ascorbic acid in metal stress resistance among various crop species, their combined effects in the context of heavy metal stressed conditions remain underexplored. Wheat (Triticum aestivum L.

Characterization of non-typhoidal Salmonella reveals the highly prevalent mcr-1-positive S. 1,4,[5],12:i:- within eggs are derived from chickens.

Salmonella is one of the most common foodborne pathogens. Antimicrobial-resistant Salmonella isolates, especially those resistant to colistin, pose a significant threat to public health worldwide. However, data about the prevalence of mcr-positive Salmonella in animals was few and the dissemination of mcr-positive Salmonella from animals to food, especially eggs, has not been fully addressed.

TNFSF12 is associated with breast cancer prognosis and immune cell infiltration.

Background: Breast cancer (BRCA) is one of the most common cancers in women and is the leading cause of cancer-related deaths in women. TNFSF12, originally a member of the TNF superfamily, is considered a key molecule that is associated with poor prognosis of many cancers. However, its role in progression of BRCA remains unclear.

Design and application of a novel 3D printing digital navigation template for cubitus varus deformity in children.

Background: This study was aimed to assess the feasibility and efficacy of 3D printing digital template for treatment of cubitus varus deformity.

Methods: 32 patients who underwent lateral closing osteotomy were evaluated between January 2018 and January 2020 in this retrospective study. Navigation templates were used in 17 cases, while conventional surgery in 15 cases.

Brain ischemia causes systemic Notch1 activity in endothelial cells to drive atherosclerosis.

Stroke leads to persistently high risk for recurrent vascular events caused by systemic atheroprogression that is driven by endothelial cell (EC) activation. However, whether and how stroke induces sustained pro-inflammatory and proatherogenic endothelial alterations in systemic vessels remain poorly understood. We showed that brain ischemia induces persistent activation, the upregulation of adhesion molecule VCAM1, and increased senescence in peripheral ECs until 4 weeks after stroke onset.

Two chromosome-level genome assemblies of galling aphids Slavum lentiscoides and Chaetogeoica ovagalla.

Slavum lentiscoides and Chaetogeoica ovagalla are two aphid species from the subtribe Fordina of Fordini within the subfamily Eriosomatinae, and they produce galls on their primary host plants Pistacia. We assembled chromosome-level genomes of these two species using Nanopore long-read sequencing and Hi-C technology. A 332 Mb genome assembly of S.

Inflammatory biomarkers may be associated with poor outcomes after mechanical thrombectomy.

Background: Mechanical thrombectomy (MT) has become the mainstay of treatment for acute ischemic stroke (AIS) recently. This case-control study aimed to identify the pivotal role of inflammation in the prognosis of AIS patients after MT.

Methods: Altogether, 70 AIS patients who underwent MT were retrospectively recruited for this study.

[Correlation between serum HDL/LDL and t-PINP /β-CTX and osteoporotic vertebral fractures in elderly women].

Objective: To explore high density lipoprotein (HDL)/low density lipoprotein (LDL) and total typeⅠcollagen amino terminal extender peptide (t-PINP)/ C-terminal peptide of typeⅠcollagen β special sequence(β-CTX)and risk of osteoporosis vertebral fractures (OPVFs) in elderly women.

Methods: The clinical data of 446 female OPVFs patients aged above 60 years old from January 2019 to December 2020 were retrospectively analyzed. According to whether or not fracture, patients were divided into non-fracture group (186 patients) and fracture group(260 patients).

Five-Year Incidence of Nd:YAG Capsulotomy After Cataract Surgery with Different Single-Piece Monofocal Intraocular Lenses: A Follow-Up Study of 3955 Eyes.

Purpose: To estimate the incidence of neodymium-doped yttrium aluminum garnet laser (Nd:YAG) capsulotomy up to five years after cataract surgery with different single-piece acrylic monofocal IOLs in a Spanish cohort.

Patients And Methods: Data were extracted from electronic medical records. Eligible participants were aged ≥65, had cataract surgery with one of five different acrylic monofocal IOLs (Alcon AcrySof, AJL LLASY60, Medicontur Bi-flex, IOL Tech Stabibag and Zeiss Asphina), and more than six months baseline data.

Genomic signatures associated with recurrent scale loss in cyprinid fish.

Scale morphology represents a fundamental feature of fish and a key evolutionary trait underlying fish diversification. Despite frequent and recurrent scale loss throughout fish diversification, comprehensive genome-wide analyses of the genomic signatures associated with scale loss in divergent fish lineages remain scarce. In the current study, we investigated genome-wide signatures, specifically convergent protein-coding gene loss, amino acid substitutions, and cis-regulatory sequence changes, associated with recurrent scale loss in two divergent Cypriniformes lineages based on large-scale genomic, transcriptomic, and epigenetic data.

Dehydration in cerebral venous sinus thrombosis.

Aims: This study aimed to unravel the dehydration status of patients with cerebral venous sinus thrombosis (CVST) to facilitate the understanding of dehydration in CVST.

Methods: This was a multicenter retrospective study and three populations were recruited, namely, patients with CVST, CVST mimics, and healthy subjects. Blood samples were obtained 1-2 days after admission to assess dehydration status.

Combined ROS Sensitive Folate Receptor Targeted Micellar Formulations of Curcumin Effective Against Rheumatoid Arthritis in Rat Model.

Introduction: Rheumatoid arthritis (RA) is an inflammatory immune-mediated disease that involves synovitis, cartilage destruction, and even joint damage. Traditional agents used for RA therapy remain unsatisfactory because of their low efficiency and obvious adverse effects. Therefore, we here established RA microenvironment-responsive targeted micelles that can respond to the increase in reactive oxygen species (ROS) levels in the joint and improve macrophage-specific targeting of loaded drugs.

Asymmetric and parallel subgenome selection co-shape common carp domestication.

Background: The common carp (Cyprinus carpio) might best represent the domesticated allopolyploid animals. Although subgenome divergence which is well-known to be a key to allopolyploid domestication has been comprehensively characterized in common carps, the link between genetic architecture underlying agronomic traits and subgenome divergence is unknown in the selective breeding of common carps globally.

Results: We utilized a comprehensive SNP dataset in 13 representative common carp strains worldwide to detect genome-wide genetic variations associated with scale reduction, vibrant skin color, and high growth rate in common carp domestication.