Facultative or obligate anaerobic bacteria have the potential for multimodality therapy of solid tumours.

Recent understanding of the unique pathology of solid tumours has shed light on the difficult and disappointing nature of their clinical treatment. All solid tumours undergo angiogenesis that results in biological changes and adaptive metabolisms, i.e.

[Treatment of complex acetabular fractures by a modified extended iliofemoral approach].

Objective: To evaluate the effect of the modified extended iliofemoral approach on treating complex acetabular fractures.

Methods: Thirty-six cases of complex acetabular fractures were treated by the open reposition and internal fixation by a modified extended iliofemoral approach.

Results: Thirty-six cases were followed up for 7-46 months, with an average of 23.

In vitro and in vivo evaluation of two rational-designed nonpeptidic farnesyltransferase inhibitors on HT29 human colon cancer cell lines.

FTase inhibitors constitute a new class of potential cancer therapeutics, especially in colorectal cancer where K-ras-selective mutations exist and have a role in tumorigenesis. The synthesis and biological evaluation of two nonpeptidic molecules (13 and 16) designed on the basis of a zinc chelator imidazole linked to two aromatic fragments able to fit in the "exit groove" and in the "A2 binding site" of FTase are described. These molecules are characterized respectively by a flexible phenylmethyl chain and a more constrained scaffold so as to evaluate their respective influences on site recognition.

[Effect of phosphate on growth and polysaccharide production by suspension cultures of protocorm-like bodies of Dendrobium huoshanense].

The effect of inorganic phosphate on cell growth, accumulation of polysaccharides together with nutrient utilization was investigated in suspension cultures of protocorm-like bodies of Dendrobium huoshanense. Thirty-day-old cells were transferred into liquid medium with the inoculum density of 100 g/L cells. The results indicate that the optimal concentration of phosphate in medium for cell growth of protocorm-like bodies of Dendrobium huoshanense was 2.

High-yield synthesis of single crystalline tin-doped indium oxide octahedrons via a low-temperature, catalyst-free process.

We report a large-scale synthesis of micron and nanosized tin-doped indium oxide (ITO) octahedrons via an ultrasonic spray assisted chemical vapour deposition, in which no catalyst is involved. The single crystalline nature of the ITO octahedrons was revealed by high-resolution transmission electron microscopy. The formation of ITO octahedrons, which are bounded by eight {111} facets, is due to the lowest surface energy of the {111} facet.

[Correlation between the expression of HER-2/neu, nm23 protein and local invasion and lymph node metastasis of human LSCC].

Objective: To explore the relationship between the expressions of HER-2/neu, nm23 protein and local invasion and lymp node metastasis of LSCC.

Method: The expression of HER-2/neu, nm23 protein was detected by immunohistochemical technique in 56 patients with human LSCC.

Result: The positive expression rate of HER-2/neu protein in LSCC was not correlated with histological grade (P > 0.

Analysis of a new homozygous deletion in the tumor suppressor region at 3p12.3 reveals two novel intronic noncoding RNA genes.

Homozygous deletions or loss of heterozygosity (LOH) at human chromosome band 3p12 are consistent features of lung and other malignancies, suggesting the presence of a tumor suppressor gene(s) (TSG) at this location. Only one gene has been cloned thus far from the overlapping region deleted in lung and breast cancer cell lines U2020, NCI H2198, and HCC38. It is DUTT1 (Deleted in U Twenty Twenty), also known as ROBO1, FLJ21882, and SAX3, according to HUGO.

Search for "weapons of mass destruction" for cancer -- immuno/ gene therapy comes of age.

The complexity of a cancer, such as cell heterogeneity, and the existence of hypoxia, stromal cells and stem cells has so far prevented successful development and treatment of patients suffering from the later stages of cancers. At present, the use of conventional therapies, such as chemo/radio therapy is limited, and only therapies that are focused on utilizing the patient's immune response to combat against the disease appear to be the most reliable and promising. Two decades ago, cytokines were discovered to be able to activate the immune systems and mount an anti-tumour response.

Reconstruction of the anophthalmic orbit by orbital osteotomy and free flap transfer.

It is a challenging problem for the plastic surgeon to perform adequate reconstruction of the postenucleation and radiated orbit of patient with retinoblastoma. Rebuilding of the orbital structure, reconstruction of the eye socket, and restoration of periorbital volume are required in this kind of orbital reconstruction. In this study, we reviewed 12 patients with hypoplastic orbital deformities who underwent orbital osteotomy and free flap transfer.

Gene transfer to dendritic cells induced a protective immunity against melanoma.

Lentiviral vectors have shown promises for efficient gene transfer to dividing as well as nondividing cells. In this study, we explored lentiviral vector-mediated, the entire mTRP-2 gene transfer and expression in dendritic cells (DCs). Adoptive transfer of DCs-expressing mTRP-2 (DC-HR'CmT2) into C57BL/6 mouse was also assessed.

[The expression and significance of Fas/FasL in nasal polyps tissue].

Objective: To explore the induction and control effect of Fas/FasL system in the occurrence and evolution of nasal polyps tissue.

Method: Nasal polyps samples of 40 cases and 5 healthy adults were examined for the genic expressions of factors Fas and FasL by using the method of reverse transcription polymerase chain reaction (RT-PCR).

Result: The results indicated that the expressions of FasL in nasal polyps was high, and the expressions of Fas was low.

[Analysis and identification of Liriomyza sativae-attractants from cowpea and kidney bean volatiles].

In this paper, the volatiles from cowpea and kidney bean, the main host plants of American leafminer Liriomyza sativae, were collected and extracted by a self-designed device and solid phase microextraction (SPME) technique, and analyzed by GC-MS. The results showed that the volatiles from the two beans had the same components mainly consisted of 2-hexenal, 3-Hexen-1-ol, 2-Hexen-1-ol, 1-Octen-3-ol, 3-Hexenol acetate, alpha-Ionone and beta-Ionone. The lure made of the seven components was attractive to L.

Silencing the epidermal growth factor receptor gene with RNAi may be developed as a potential therapy for non small cell lung cancer.

Lung cancer has emerged as a leading cause of cancer death in the world. Non-small cell lung cancer (NSCLC) accounts for 75-80% of all lung cancers. Current therapies are ineffective, thus new approaches are needed to improve the therapeutic ratio.

Dopamine D(4) receptor antagonist 3-(4-[(18)F]fluorobenzyl)-8-methoxy-1,2,3,4-tetrahydrochromeno[3,4-c]pyridin-5-one([(18)F]FMTP): radiosynthesis and in vivo characterization in rats.

We synthesized a novel (18)F-labeled dopamine D(4) receptor antagonist (Ki=4.3 nM), 3-(4-[(18)F]fluorobenzyl)-8-methoxy-1,2,3,4-tetrahydrochromeno[3,4-c]pyridin-5-one ([(18)F]FMTP), which has exhibited high affinity and selectivity. Radiosyntheses were accomplished by the reaction of fluorine-18-labeled intermediate with 8-methoxy-1,2,3,4-tetrahydrochromeno[3,4-c]pyridin-5-one (1) followed by HPLC purification.

[Correction of hypoplastic orbit after eradiation therapy with the technique of ostectomy and flap transplantation].

Objective: To introduce an effective method for reconstruction of hypoplastic orbit caused by eradiation therapy.

Methods: The orbital reestablishment was carried out by using the orbital ostectomy to enlarge the orbital cavity and a flap transferring for the socket reconstruction and the repair of the concave deformity around the orbit.

Results: Twelve treated patients demonstrated the satisfactory improvement.

A direct quantitative analysis method for monitoring biogenic volatile organic compounds released from leaves of Pelargonium hortorum in situ.

A direct quantitative method is presented that is based upon the use of multiple headspace solid phase microextraction (HS-SPME) to monitor biogenic volatile organic compounds (BVOCs) released from a living leaf of Pelargonium hortorum in situ. Seventeen BVOCs were detected by GC-MS after a single SPME extraction using a CAR/DVB/PDMS fibre. An internal standard was employed to determine the absolute amounts of seven terpenoid compounds released from a P.

Cytostatic and pro-apoptotic effects of a novel phenylacetate-dextran derivative (NaPaC) on breast cancer cells in interactions with endothelial cells.

We have tested a novel hybrid molecule made of carboxymethylbenzylamide dextran (CMDB) and sodium phenylacetate (NaPa) groups, called the CMDB-NaPa ester (NaPaC), on the proliferation of breast cancer and endothelial cells as well as paracrine effects between these two cell types. Our results showed that NaPaC inhibited the proliferation of MDA-MB-231 cells and MCF-7 cells in a dose-dependent manner. NaPaC was 20-fold more active on highly invasive MDA-MB-231 cells than the NaPa parental molecule.

The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer events.

BACKGROUND: Although lentiviral vectors have been widely used for in vitro and in vivo gene therapy researches, there have been few studies systematically examining various conditions that may affect the determination of the number of viable vector particles in a vector preparation and the use of Multiplicity of Infection (MOI) as a parameter for the prediction of gene transfer events. METHODS: Lentiviral vectors encoding a marker gene were packaged and supernatants concentrated. The number of viable vector particles was determined by in vitro transduction and fluorescent microscopy and FACs analyses.

[Development of software system of medical monitoring equipment in spacecraft].

Objective: To develop a software system that can gather, manage and transmit signals of electrocardiograms, respiration, body temperature and blood pressure at real time, in order to monitor physiological signals of astronauts.

Method: Based on single-chip microprocessor, software system completed the A/D conversion, real-time signal processing, storage and transmission.

Result: The software system realized data acquisition of electrocardiogram, respiration, body temperature and blood pressure, as well as heart rate extraction from electrocardiogram, storage of physiological data, and transmission of physiological parameters to spacecraft.

Sellmeier's equation and the expression of the thermal refractive-index coefficient for a Nd0.007Gd0.993VO4 crystal.

The principal refractive indices of a Nd0.007Gd0.993VO4 crystal for wavelengths of 0.

[A molecular epidemiological study on the human immunodeficiency virus infection in Jiangsu province].

Objective: To identify subtypes of human immunodeficiency virus 1 (HIV-1) strains and their distribution, infection sources, and the trends of HIV infection in Jiangsu province.

Methods: Anticongulated bloods from 46 infected persons were collected to separate previrus DNA. HIV-1 env genes were then amplified by nested-PCR and sequenced for their C2-V3 region so as to identify subtypes.