Enzymatic Synthesis of Poly(β-Amino Ester) Copolymer With High Potency in Eliminating Gram-Negative Bacteria.

The functional lactone 2-Methyl-2-propanyl-7-oxo-1,4-oxazepane-4-carboxylate (BocOP), containing a protected secondary amino group, is synthesized through oxone-mediated Baeyer-Villiger oxidation. After that, the homopolymerization kinetics of BocOP catalyzed by immobilized Candida antarctia lipase B (Novozym 435, N435) are investigated. The N435 can efficiently catalyze the conversion of BocOP into poly(β-amino ester) (PAE).

Evaluation of Salivary Carcinogenic microR-21 and miR-125a Expression Associated with Alcohol Consumption and Smoking.

Objective: The concept of "lifestyle" encompasses various factors, including nutrition, behavior, stress, physical activity, work habits, smoking, and alcohol consumption. Increasing evidence suggests that environmental and lifestyle factors can influence epigenetic mechanisms, such as DNA methylation, histone acetylation, and microRNA expression. Given that microRNAs (miRNAs) are an emerging focus in cancer research, there is growing interest in understanding how lifestyle choices affect miRNA responses.

Thrombus iodine-based perviousness is associated with recanalisation and functional outcomes in endovascular thrombectomy.

Background: Dual-energy CT (DECT) provides several novel methods to assess thrombus perviousness. We aimed to evaluate whether the novel thrombus perviousness measured with DECT is associated with improved recanalisation and better functional outcomes in acute ischaemic stroke (AIS) patients with endovascular thrombectomy (EVT).

Methods: 108 AIS patients with middle cerebral artery occlusion who underwent DECT angiography on admission and received EVT treatment between April 2020 and September 2023 were retrospectively analysed.

A three-stage method for analyzing market concentration and airport efficiency in the Yangtze river Delta.

With ongoing development of ground transportation system, competing regional airports need to collaborate in order to improve their efficiency and passenger turnover. However, finding the most advantageous dynamic connection between airports' rivalry and collaboration, passenger market alterations, and influencing factors is quite challenging. To this end, our study proposed a three-stage method combining analytical methodologies and data envelopment analysis (DEA) to analyze the changing trend and correlation of passenger market concentration as well as operational efficiency in ten airports of China's Yangtze River Delta (YRD) airport group during period from 2011 to 2019, China.

[Functions and mechanisms of autophagy-related genes in plant responses to adversity stresses].

Autophagy is an evolutionarily conserved self-degradation process in eukaryotes. It not only plays a role in plant growth and development but also is involved in plant responses to biotic and abiotic stresses. Plants can initiate autophagy to degrade the surplus or damaged cytoplasmic materials and organelles, thus coping with abiotic and biotic stresses.

[Functions and mechanisms of Zn-dependent histone deacetylase in plant responses to abiotic stress].

The HDAs (a subfamily of histone deacetylases), a class of Zn-dependent histone deacetylases, are highly homologous to the reduced potassium dependency 3 (RPD3) in yeast. HDAs extensively regulate chromosome stability, gene transcription, and protein activity by catalyzing the removal of acetyl group from histone and non-histone lysine residues. HDA-mediated deacetylation is essential for plant growth, development, and responses to abiotic stress.

Synthesis of PbCrO nanorods-TiCT MXene composites: A sensitive photoelectrochemical sensor for the detection of cysteine in human blood serum.

Background: l-cysteine plays an important role in protein synthesis, osmoregulation, detoxification, nervous system function, and antioxidant process. Low levels of cysteine are associated with various diseases like cardiovascular diseases, ischemic stroke, neurological disorders, diabetes, cancer like lung and colorectal cancer, renal dysfunction-linked conditions, and vitiligo. Therefore, it is of great significance to develop a rapid and accurate method for the determination of cysteine concentration.

Self-Healing, Degradable, and Biobased Polyurethane Elastomer for High-Performance Piezoresistive Pressure Sensors with a Hump-like Microstructure.

Flexible sensors are widely applied in the fields of electronic skins and wearable devices, yet it is still a big challenge to effectively prolong the lifespan of the damaged sensors and reduce environmental pollution caused by discarded sensors after updating and upgrading. Herein, we proposed a self-healing, degradable, and biobased polyurethane elastomer for high-performance flexible pressure sensors. The elastomer synthesized using fatty diamine as a chain extender possessed a high tensile strength of 13.

Genomic epidemiology and antimicrobial resistance of clinical isolates between 2016 and 2023.

is a Gram-negative, opportunistic pathogen that is often associated with nosocomial infections. Here, the genomic characteristics and antimicrobial resistance (AMR) of clinical isolates between 2016 and 2023 were determined. A total of 218 clinical isolates were mainly identified from urinary tract (48.

Transcriptome analysis reveals the molecular mechanism of γ-linolenic acid eradicating the biofilm of vancomycin-resistant .

Introduction: Vancomycin-resistant (VRE-fm) biofilms pose a significant clinical challenge due to the limited effectiveness of traditional antibiotics. This study investigates the potential of γ-linolenic acid (GLA) as a novel antibiofilm agent.

Methods: Transcriptome analysis was performed on the V27 isolate, comparing cells in mature biofilms treated with and without GLA.

Normobaric hyperoxia combined with endovascular treatment for acute ischaemic stroke in China (OPENS-2 trial): a multicentre, randomised, single-blind, sham-controlled trial.

Background: Endovascular treatment improves the recanalisation rate for patients with acute ischaemic stroke; however, even with endovascular treatment, approximately half of patients do not have a favourable functional outcome. We aimed to evaluate the effect of normobaric hyperoxia combined with endovascular treatment on functional outcomes up to 90 days after treatment in patients who had an acute ischaemic stroke with large-vessel occlusion.

Methods: In this multicentre, randomised, single-blind, sham-controlled trial, patients aged 18-80 years presenting within 6 h of acute ischaemic stroke attributed to large-vessel occlusion in anterior circulation, who were candidates for endovascular treatment, were recruited from 26 comprehensive stroke centres in China.

The efficacy of telemedicine interventions on quality of life and depression in individuals with spinal cord injury: a systematic review and meta-analysis.

Objective: The purpose of this systematic review and meta-analysis is to examine the impact of telemedicine interventions on the quality of life (QoL) and depression in individuals with spinal cord injury (SCI).

Methods: A literature search was conducted in four electronic databases (PubMed, Web of Science, EMBASE, and the Cochrane Library) from their inception to February 1, 2024. Two authors independently screened the studies and extracted the data.

Isolation, structural characterization of natural chondroitin sulfate oligosaccharides and their binding study with anti-angiogenic factors.

Drugs that inhibit tumor angiogenesis, promote vascular normalization and improve the tumor microenvironment. However, their application is limited by adaptive or compensatory resistance. Chondroitin sulfate (CS) regulates numerous proteins including pro-angiogenic growth factors, for whom binding affinity depends on sulfation of CS.

MgSO as a novel hypothermia infusion solution promotes ischemic stroke recovery through Ca regulation of neurovascular units.

The neuroprotection of acute ischemic stroke (AIS) patients to alleviate the reperfusion injury is a long-standing problem. Although the intra-arterial selective cooling saline infusion (IA-SCSI) is a promising technique to improve the outcome of AIS, the clinical results are not sufficiently satisfactory. Magnesium-containing solutions such as magnesium sulfate (MgSO) can protect cells by modulating the intracellular Ca content in damaged cells, suggesting that they could be hypothermia infusion solution.

Discovery of Novel Diagnostic Biomarkers for Common Pathogenic Through Pan-Genome and Comparative Genome Analysis, with Preliminary Validation.

The aim of this study was to reveal diagnostic biomarkers of considerable importance for common pathogenic , utilizing pan-genomic and comparative genome analysis to accurately characterize clinical infections. In this study, complete or assembled genome sequences of common pathogenic and closely related species were obtained from NCBI as discovery and validation sets, respectively. Genome annotation was performed using Prokka software, and pan-genomic analysis and extraction of core genes were performed using BPGA software.

Analysis of gradual diameter thrombectomy stent .

Objective: To analyze the effect of gradient thrombectomy stent .

Methods: The cerebrovascular fluid circulation model was made and fixed on the test table. About 0.

Sprouting sympathetic fibres release CXCL16 and norepinephrine to synergistically mediate sensory neuronal hyperexcitability in a rodent model of neuropathic pain.

Background: Chronic neuropathic pain generally has a poor response to treatment with conventional drugs. Sympathectomy can alleviate neuropathic pain in some patients, suggesting that abnormal sympathetic-somatosensory signaling interactions might underlie some forms of neuropathic pain. The molecular mechanisms underlying sympathetic-somatosensory interactions in neuropathic pain remain obscure.

Eicosapentaenoic acid as an antibiofilm agent disrupts mature biofilms of .

The biofilm formation of , a major human fungal pathogen, represents a crucial virulence factor during candidiasis. Eicosapentaenoic acid (EPA), a polyunsaturated fatty acid, has emerged as a potential antibiofilm agent against . .

New strategy for the utilization of invasive species: A tert-butylhydroquinone electrochemical platform based on Solidago canadensis L.

Tert-butylhydroquinone (TBHQ) is a widely used synthetic phenolic antioxidant found in edible oils and other fried foods. Nevertheless, the excess use of TBHQ can reduce food quality and impact public health. In this paper, we reported the synthesis of a nanocomposite consisting of carbon and nitrogen co-doped nickel oxide (NiO-N/C-700), which was used to modify a pencil graphite electrode for the sensitive detection of TBHQ.

Development and validation of a novel model to predict post-stroke cognitive impairment within 6 months after acute ischemic stroke.

Background: Cognitive decline following acute ischemic stroke (AIS), termed post-stroke cognitive impairment (PSCI), is a prevalent phenomenon that significantly elevates disability and mortality rates among affected patients. The objective of this investigation was to develop a robust clinical prediction model capable of forecasting PSCI within six months post-AIS and subsequently validate its effectiveness.

Methods: A cohort of 573 AIS patients was stratified into two groups: those with PSCI (260 cases) and those who remained cognitively normal (CN) (313 cases).

A scoping review of digital technology applications in palliative care.

Background: In the current era of rapid technological development, digital technology is progressively transforming the medical field. In palliative care, its integration is an inevitable trend, offering new possibilities for improving care delivery and patient outcomes.

Objective: This study aimed to review the application of digital technologies in palliative care, focusing on their advantages, challenges, and measurable impacts across diverse care settings.

Bacterial ghosts: A breakthrough approach to cancer vaccination.

Cancer is a devastating disease worldwide with high mortality rates and is a foremost concern for society. Immunotherapy has emerged as a promising strategy for treating cancer, harnessing the power of immune system to recognize and kill tumor cells. Bacterial ghosts (BGs), a novel platform in cancer vaccination, are suitable for personalized and effective immunotherapeutic interventions.

Effects of intravenous anesthesia and inhalation anesthesia on postoperative inflammatory markers in patients with esophageal cancer: a retrospective study.

Background: Inflammatory markers have been confirmed to be associated with the prognosis of cancer patients. In this study, we compared the impacts of intravenous anesthesia and inhalation anesthesia on the neutrophil-to-lymphocyte ratio (NLR), the platelet-to-lymphocyte ratio (PLR), and the systemic immune-inflammation index (SII) after esophageal cancer surgery.

Methods: We retrospectively reviewed the electronic medical records of patients who underwent esophagectomy from January 1, 2014 to December 31, 2016.

Association between stroke subtypes and outcomes of endovascular therapy: a post-hoc analysis of the ANGEL-ASPECT Trial.

Objectives: Our study aims to examine the value of endovascular therapy (EVT) and its comparison to medical management (MM) in ischaemic stroke patients accompanied by large artery atherosclerosis (LAA) and non-LAA METHODS: modified Rankin scale score (mRS) was evaluated at 90 days post the stroke attack and was considered as the primary outcome. Other outcomes measured in this study included score changes of 0-2 and 0-3 on the mRS. The occurrence of symptomatic intracranial haemorrhage at 24 hours after EVT was also measured as a safety endpoint.

Edaravone Protects Trophoblast Cells From Hypoxic Injury in Preeclampsia: Inhibition of the PI3K/AKT Pathway as a Promising Therapeutic Approach.

Objective: Preeclampsia (PE) is a multifaceted medical condition that manifests during pregnancy, characterized by hypertension and damage to multiple organs. In PE, the placenta's impaired functionality leads to continuous hypoxia in placental tissues, which is considered the primary cause of the condition. Inhibition of hypoxia-induced injury in trophoblast cells presents a potential therapeutic strategy for PE.