Engineered circular RNA-based DLL3-targeted CAR-T therapy for small cell lung cancer.

Purpose: Circular RNA (circRNA) has emerged as a promising RNA therapeutic molecule due to its enhanced stability and prolonged protein expression compared to messenger RNA (mRNA). Using circRNA to construct transient Chimeric Antigen Receptor (CAR)-T cells can mitigate the limitations of conventional viral vector-based CAR-T approaches, such as complex process and long-term side effects.

Methods: The study first reconfirmed the advantageous properties of circRNA, focusing on its stability and protein expression efficiency.

[Relationships between Molecular Genetics and Clinical Features of Children with Acute Myeloid Leukemia].

Objective: To analyze the molecular genetic spectrum of children with acute myeloid leukemia (AML), and explore its correlation with clinical characteristics and prognosis.

Methods: The clinical and molecular genetic data of 116 children with newly diagnosed AML in Wuhan Children's Hospital from September 2015 to August 2022 were retrospectively analyzed. The Fisher's exact test was used to analyze the correlation of gene mutations with clinical features, and Kaplan-Meier curve was used to analyze the influences of gene mutations on the prognosis.

Predicting the spatial demand for public charging stations for EVs using multi-source big data: an example from jinan city, china.

Under the pressure of carbon pollution and resource scarcity, electric vehicles (EVs) have gradually replaced fuel vehicles as a new trend of low-carbon transformation. However, public charging stations (PCS) face with problems such as insufficient quantity and unreasonable distribution. By using multi-source big data, this paper analyzes the population distribution, traffic organization, infrastructure, land use and regional economy of Jinan urban area, China, and constructs a comprehensive evaluation index system to predict the spatial demand of PCS for EVs.

ECE-CYC1 Transcription Factor CmCYC1a May Interact with CmCYC2 in Regulating Flower Symmetry and Stamen Development in .

Background: The attractive inflorescence of , its capitulum, is always composed of ray (female, zygomorphy) and disc (bisexual, actinomorphy) florets, but the formation mechanism remains elusive. The gene diversification pattern of the ECE (CYC/TB1) clade has been speculated to correlate with the capitulum. Within the three subclades of ECE, the involvement of CYC2 in defining floret identity and regulating flower symmetry has been demonstrated in many species of Asteraceae, including .

Enhanced Exciton Delocalization in Organic Near-Infrared Photodetectors via Solid Additive-Mediated J-Aggregation.

Near-infrared organic photodetectors (NIR-OPDs) have emerged as increasingly significant in optoelectronics, offering unparalleled advantages for applications in health monitoring and night vision. The development of self-powered devices with low dark currents and enhanced NIR sensitivity involves complex engineering that requires careful material selection, defect state density control, and environmental consideration. In this study, an innovative approach is introduced that utilizes solid additive (DIB) to induce improvements in the J-aggregation morphology and exciton delocalization in acceptor molecules.

Activated mTOR Signaling in the RPE Drives EMT, Autophagy, and Metabolic Disruption, Resulting in AMD-Like Pathology in Mice.

The mechanistic target of rapamycin (mTOR) complexes 1 and 2 (mTORC1/2) are crucial for various physiological functions. Although the role of mTORC1 in retinal pigmented epithelium (RPE) homeostasis and age-related macular degeneration (AMD) pathogenesis is established, the function of mTORC2 remains unclear. We investigated both complexes in RPE health and disease.

Combining 3D printing technology with customized metal plates for the treatment of complex acetabular fractures: A retrospective study.

Purposes: The purpose of this study is to evaluate the clinical outcomes of combining 3D printing technology with customized metal plates in the treatment of complex acetabular fractures.

Methods: A retrospective study was conducted on 42 patients with complex acetabular fractures treated at our hospital between September 1, 2020 and May 31, 2022. The patients were divided into two groups: the conventional group and the 3D printing group, with 21 individuals in each group.

Bacillus thuringiensis: a gift for nematode management.

Nematodes attacking plants and animals pose a global threat to agricultural industry and public health. Chemicals as long preferred tools for nematode management are facing challenges such as pest resistance and policy restrictions. Recent findings show that Bacillus thuringiensis (Bt) produces rich components with excellent nematicidal competence and is a precious nonchemical resource for controlling a broad range of nematode parasites.

Subcutaneous depth-selective spectral imaging with mμSORS enables noninvasive glucose monitoring.

Noninvasive blood glucose monitoring offers substantial advantages for patients, but current technologies are often not sufficiently accurate for clinical applications or require personalized calibration. Here we report multiple μ-spatially offset Raman spectroscopy, which captures Raman signals at varying skin depths, and show that it accurately detects blood glucose levels in humans. In 35 individuals with or without type 2 diabetes, we first determine the optimal depth for glucose detection to be at or below the capillary-rich dermal-epidermal junction, where we observe a strong correlation between specific Raman bands and venous plasma glucose concentrations.

Neutralizing antibody landscape of the non-polio Enteroviruses and future strategy.

The non-polio Enteroviruses (NPEVs), consist of enteroviruses, coxsackieviruses, echoviruses, and rhinoviruses, are causative agents for a wide variety of diseases, ranging from common cold to encephalitis and acute flaccid paralysis (AFP). In recent years, several NPEVs have become serious public health threats, include EV-A71, which has caused epidemics of hand-foot-and-mouth disease (HMFD) in Southeast Asia, and EV-D68, which caused outbreaks of severe respiratory disease in children worldwide. Infections with these viruses are associated with neurological diseases like aseptic meningitis and AFP.

Integrative multi-omics analysis reveals the translational landscape of the plant-parasitic nematode Meloidogyne incognita.

Root-knot nematodes (RKNs) of the genus Meloidogyne pose the most significant threats to global food security due to their destructive nature as plant-parasitic nematodes. Although significant attention has been devoted to investigating the gene transcription profiling of RKNs, our understanding of the translational landscape of RKNs remains limited. In this study, we elucidated the translational landscape of Meloidogyne incognita through the integration of translatome, transcriptome and quantitative proteome analyses.

Relationship between the and the gene copy number variation and growth traits in different cattle breeds.

Copy number variations (CNV) are important genetic variations. The endogenous factors cobalamin receptor () and MIA SH3 domain ER-derived factor 3 () are associated with bone/muscle development and intramuscular fat deposition. There have been no reports on the effects of and CNVs on growth traits of Chinese cattle.

Surgical options for Evans-Jensen type IV intertrochanteric femur fractures in the elderly over 65: a comparison between total hip arthroplasty and proximal femoral nail antirotation.

Background: Currently, there is no clear standard for the surgical options for Evans-Jensen Type IV intertrochanteric femur fractures in elderly patients over 65 years old. This study aims to retrospectively analyze and compare the early postoperative limb function and quality of life of patients treated with total hip arthroplasty (THA) and proximal femoral nail antirotation (PFNA) for this type of fracture. We hypothesize that there is no significant difference in complications and postoperative recovery between the two surgical methods.

Rapid Determination of Water-Soluble Vitamins in Human Serum by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry.

Water-soluble vitamins play essential roles in normal body functions and metabolic activities. However, few methods have simultaneously measured all nine water-soluble vitamins in biological matrices. In this study, we developed a sensitive and accurate method for the simultaneous measurement of thiamine (B1), riboflavin (B2), nicotinamide (B3), pantothenic acid (B5), 4-pyridoxic acid (B6), biotin (B7), 5-methyltetrahydrofolic acid (B9), ascorbic acid (VC), and methylmalonic acid (MMA) in human serum.

A comparative study on the efficacy of kirschner wire tension band combined with anchor cross-suture internal fixation versus partial patellectomy in the treatment of comminuted inferior pole patellar fractures.

Objective: The treatment of comminuted inferior pole patellar fractures has long posed a challenge for orthopedic surgeons. This study aims to compare the biomechanical stability and clinical efficacy of Kirschner wire tension band combined with anchor cross-suture fixation versus traditional partial patellectomy in the treatment of comminuted inferior pole patellar fractures.

Methods: A retrospective analysis was conducted on 14 patients who underwent Kirschner wire tension band combined with anchor cross-suture fixation (Group A) in our department of orthopedics from September 2020 to April 2022.

Vertebral Augmentation plus Pedicle Screw Fixation versus Vertebral Augmentation Alone in the Treatment of Osteoporotic Thoracolumbar Fractures: A Meta-Analysis.

Background: This meta-analysis was conducted to compare the efficacy and safety of vertebral augmentation (VA) plus pedicle screw fixation (PSF) with VA for treating osteoporotic thoracolumbar fractures (OTLFs).

Methods: A comprehensive search was conducted in PubMed, Embase, Cochrane Library, and CNKI (China National Knowledge Infrastructure) to identify studies comparing PSF + VA with VA for treating OTLF. The primary outcomes were operation time, blood loss, length of stay, visual analog scale (VAS) score, Oswestry Disability Index (ODI), Cobb angle, anterior vertebral height (AVH), bone cement leakage, secondary fracture, and other adverse events.

Baseline and cumulative Chinese visceral adiposity index and diabetic kidney disease: A prospective cohort study.

Background: Diabetic kidney disease (DKD) makes up nearly half of all chronic kidney disease cases and is a major cause of mortality for people with diabetes. However, the study of the association of longitudinal Chinese visceral adiposity index (CVAI) with DKD is still missing.

Methods: This prospective cohort study included 7874 diabetes patients from the Kailuan study.

Chinese Visceral Adiposity Index Trajectory and Stroke in Prediabetes and Diabetes: A Prospective Cohort Study.

Aims: Stroke is a common diabetic complication, by which the Chinese visceral adiposity index (CVAI) is confirmed as a better predictor of visceral fat. However, the relationship between CVAI change and the stroke risk among patients with diabetes and prediabetes remains unclear. Therefore, we aimed to examine the association of CVAI trajectory with the risk of stroke.

Bile acid synthesis impedes tumor-specific T cell responses during liver cancer.

The metabolic landscape of cancer greatly influences antitumor immunity, yet it remains unclear how organ-specific metabolites in the tumor microenvironment influence immunosurveillance. We found that accumulation of primary conjugated and secondary bile acids (BAs) are metabolic features of human hepatocellular carcinoma and experimental liver cancer models. Inhibiting conjugated BA synthesis in hepatocytes through deletion of the BA-conjugating enzyme bile acid-CoA:amino acid -acyltransferase (BAAT) enhanced tumor-specific T cell responses, reduced tumor growth, and sensitized tumors to anti-programmed cell death protein 1 (anti-PD-1) immunotherapy.

Causal relationship between hippocampal subfield volume and alzheimer's disease: a mendelian randomization study.

Background And Objective: Numerous studies suggest that the development of Alzheimer's Disease (AD) leads to a reduction in overall hippocampal volume. However, there is limited research exploring whether pre-morbid differences in hippocampal volume impact the risk of AD. This study aims to delve into the causal relationship between hippocampal subregional volume and AD using bidirectional Mendelian Randomization (MR) methods.

The clinical indexes and immunological status of HIV/AIDS patients undergoing different highly active antiretroviral treatments.

Objective: This study aims to investigate the differences of clinical indices in HIV patients between three different first-line antiretroviral treatment strategies in Yunnan Province, China. Furthermore, the hematologic system, liver function, kidney function, blood lipid levels of HIV patients and its association with CD4+ count, CD8+ count, CD4/CD8 ratio and antiretroviral treatment were also assessed.

Methods: This retrospective cohort study included 81 participants who underwent highly active antiretroviral treatment from September 2009 to September 2019.

Cesium accumulation and plant growth promotion characteristics of A10 isolated from L. rhizosphere soil.

The combined microbial-plant remediation has increasingly been used to remediate heavy metal-contaminated soil. Some microorganisms could enhance phytoremediation efficiency by solubilizing heavy metal and improve plant growth by producing phytohormones in the heavy metal contaminated soils. In the present study, a strong cesium (Cs)-tolerant fungal strain was identified from soil microorganisms contaminated with Cs, and the enrichment conditions for Cs were optimized.

Clinical significance of dynamic monitoring of EVI1 gene expression in pediatric acute myeloid leukemia.

Objective: To investigate the clinical significance of dynamic monitoring ecotropic virus integration site-1 (EVI1) expression in childhood acute myeloid leukemia (AML).

Methods: A retrospective analysis was conducted on 113 pediatric AML patients of Wuhan Children's Hospital from 2014 to 2022. The correlation between EVI1 expression levels and clinical indicators including clinical characteristics, first complete remission (CR1), relapse, and overall survival (OS) was analyzed.