Survival study and clinicopathological evaluation of trichilemmal carcinoma.

Trichilemmal carcinoma (TC) is a rare malignancy, commonly located on the scalp, forehead and neck, trunk or the upper extremities. The aim of this study was to investigate the clinicopathological characteristics and prognostic factors of TC, and to determine an optimal treatment strategy for these patients. Consecutive patients who were admitted to the Sun Yat-sen University Cancer Center between 1998 and 2012 were included in this retrospective study.

The neural correlates of risk propensity in males and females using resting-state fMRI.

Men are more risk prone than women, but the underlying basis remains unclear. To investigate this question, we developed a trait-like measure of risk propensity which we correlated with resting-state functional connectivity to identify sex differences. Specifically, we used short- and long-range functional connectivity densities to identify associated brain regions and examined their functional connectivities in resting-state functional magnetic resonance imaging (fMRI) data collected from a large sample of healthy young volunteers.

Comparison of emergent versus elective laparoscopic common bile duct exploration for patients with or without nonsevere acute cholangitis complicated with common bile duct stones.

Background: Laparoscopic common bile duct exploration (LCBDE) has already been established for the treatment of patients with common bile duct stones (CBDS) in elective situations. However, the effect of emergent LCBDE on those patients with nonsevere acute cholangitis has not been assessed. The aim of this study was to evaluate the effect of emergent LCBDE on patients with nonsevere acute cholangitis complicated with CBDS.

The gene expression profile of peripheral blood mononuclear cells from EV71-infected rhesus infants and the significance in viral pathogenesis.

Enterovirus 71 (EV71) is the major pathogen responsible for fatal hand, foot and mouth disease (HFMD). Our previous work reported on an EV71-infected rhesus monkey infant model that presented with histo-pathologic changes of the central nervous system (CNS) and lungs. This study is focused on the correlated modulation of gene expression in the peripheral blood mononuclear cells (PBMCs) from EV71-infected rhesus monkey infants.

Endoscopic approach to the trigeminal nerve: an anatomic study.

Objective: To describe an endoscopic perspective of the surgical anatomy of the trigeminal nerve.

Methods: Nine adult cadaveric heads were dissected endoscopically.

Results: Opening the pterygopalatine fossa is important because many key anatomical structures (V2, pterygopalatine ganglion, vidian nerve) can be identified and traced to other areas of the trigeminal nerve.

Oxytocin via its receptor affects restraint stress-induced methamphetamine CPP reinstatement in mice: Involvement of the medial prefrontal cortex and dorsal hippocampus glutamatergic system.

Our previous study revealed that intracerebroventricular oxytocin (OT) markedly inhibited the restraint stress-priming conditioned place preference (CPP) reinstatement induced by methamphetamine (MAP) via the glutamatergic system. In this study, the effect of microinjection with OT into mesocorticolimbic regions, the medial prefrontal cortex (mPFC) and the dorsal hippocampus (DHC), on the restraint stress-priming CPP reinstatement were further studied. The results showed that a 15-min restraint stress significantly reinstated MAP-induced CPP, which was inhibited by the microinjection of OT (0.

Brainnetome-wide association studies in schizophrenia: the advances and future.

Convergent evidence suggests that psychiatric disorders are the result of faulty brain networks. To understand the pathophysiological network mechanisms of psychiatric disorders, it is necessary to integrate multi-level network features obtained using various functional and anatomical brain imaging technologies on different scales. We have proposed a new concept, the brainnetome, to represent this integrative framework.

Identification of protective immunogens from extracellular secretome of Edwardsiella tarda.

Edwardsiella tarda is an opportunistic pathogen that causes a great loss in aquaculture. Identification of immune protective immunogens is a key step for development of subunit vaccines and control of the infectious diseases caused by the bacterium. This study aims to identify the protective antigens from extracellular secretory proteome of E.

Domain wall and bifurcation analysis of the Klein-Gordon Zakharov equation in (1+2)-dimensions with power law nonlinearity.

This paper studies the Klein-Gordon Zakharov equation with power law nonlinearity in (1+2)-dimensions. The ansatz method will be applied to obtain the 1-soliton solution, also known as domain wall solution, along with several constraint conditions that naturally fall out. Subsequently, the bifurcation analysis is carried out where the phase portrait is given.

An in situ formed Pd nanolayer as a bifunctional catalyst for Li-air batteries in ambient or simulated air.

We demonstrate for the first time that a Pd nanolayer, which is in situ formed on the Ni foam via the galvanic exchange method, greatly improves the energy output, the round-trip efficiency and the cyclability of the aprotic Li-air battery in ambient or simulated air.

Childhood maltreatment is associated with larger left thalamic gray matter volume in adolescents with generalized anxiety disorder.

Background: Generalized anxiety disorder (GAD) is a common anxiety disorder that usually begins in adolescence. Childhood maltreatment is highly prevalent and increases the possibility for developing a variety of mental disorders including anxiety disorders. An earlier age at onset of GAD is significantly related to maltreatment in childhood.

Aberrant default mode functional connectivity in early onset schizophrenia.

Background: The default mode network (DMN) has been linked to a number of mental disorders including schizophrenia. However, the abnormal connectivity of DMN in early onset schizophrenia (EOS) has been rarely reported.

Methods: Independent component analysis (ICA) was used to investigate functional connectivity (FC) of the DMN in 32 first-episode adolescents with EOS and 32 age and gender-matched healthy controls.

[Laryngeal reconstruction by complex hyoid bone flap after frontal partial laryngectomy].

Objective: The aim of this study was to describe a new technique of combined hyoid bone and thyrohyoid membrane flap in laryngeal reconstruction after tumor resection, and to evaluate outcome.

Methods: Six patients requiring an frontal partial laryngectomy for cancer were enrolled between September 2008 and August 2012.

Results: Nasogastric feeding was initiated within 24 hours.

Site-specific gene targeting using transcription activator-like effector (TALE)-based nuclease in Brassica oleracea.

Site-specific recognition modules with DNA nuclease have tremendous potential as molecular tools for genome targeting. The type III transcription activator-like effectors (TALEs) contain a DNA binding domain consisting of tandem repeats that can be engineered to bind user-defined specific DNA sequences. We demonstrated that customized TALE-based nucleases (TALENs), constructed using a method called "unit assembly", specifically target the endogenous FRIGIDA gene in Brassica oleracea L.

Treatment and prognosis in sinonasal mucosal melanoma: A retrospective analysis of 65 patients from a single cancer center.

Background: The purpose of this study was to evaluate the outcome in sinonasal mucosal melanoma (SMM).

Methods: A retrospective analysis of clinicopathological data from January 1976 to December 2005 was performed. Survival curve, univariate, and multivariate analyses were undertaken.

Galectin-3 accelerates the progression of oral tongue squamous cell carcinoma via a Wnt/β-catenin-dependent pathway.

The purpose of this study was to elucidate the clinicopathological significance and mechanism of action of galectin-3 in oral tongue squamous cell carcinoma (OTSCC). Here, the expression of galectin-3 was quantified in OTSCC (n = 68) and paired OTSCC and normal surrounding tissues (n = 10) using immunohistochemical staining. Tca8113 OTSCC cells were transfected with a plasmid expressing galectin-3 cDNA or siRNA against galectin-3.

Modest fructose beverage intake causes liver injury and fat accumulation in marginal copper deficient rats.

Objective: Dietary fructose and copper interaction may play an important role in the pathogenesis of nonalcoholic fatty liver disease. In this study, whether or not modest fructose consumption (3% fructose, w/v) (which is more closely related to the American lifestyle with regard to sugar beverage consumption) affects copper status, and causes liver injury and fat accumulation in marginal copper deficient rats was investigated.

Design And Methods: Male weanling Sprague-Dawley rats were fed either an adequate copper (6 ppm) or a marginally copper deficient (1.

The spectroscopy analyses of PpIX by ultrasound irradiation and its sonotoxicity in vitro.

Protoporphyrin IX (PpIX) has been used as a sensitizer in photodynamic therapy (PDT) as well as in sonodynamic therapy (SDT). The photo-bleaching of PpIX has been well investigated in many experimental systems and some photo-products have also been identified in PDT. But until now, little information has been reported about the sono-damage of PpIX in SDT.

Irradiation of the recipient site does not adversely affect successful free flap transfer in the repair of head and neck defects after salvage surgery for recurrent nasopharyngeal carcinoma originally treated with radiotherapy.

Irradiation of the recipient site has been associated with postoperative complications and an increased rate of flap failure in facial reconstruction. The aim of this study was to report the outcomes of our use of four different types of flaps (two free and two pedicled) for the reconstruction of patients with nasopharyngeal carcinoma (NPC) treated with radiotherapy, who subsequently received salvage surgery after the development of recurrence or secondary primary tumours. The records of 12 NPC patients who underwent salvage surgery and reconstruction from 2002-2007 were retrospectively reviewed.

[Apoptosis-inducing effect of annexin A2 on multiple myeloma cells and its related mechanisms].

This study was purposed to investigate the apoptosis-inducing effect of Annexin A2 gene (AnxA2) on multiple myeloma (MM) cells and its mechanisms. The human MM cell lines U266 and RPMI8226 were transfected by using siRNA targeting at AnxA2; the expressions of AnxA2 mRNA and protein in the siRNA-transfected cells were detected by real-time PCR and Western blot, respectively; the cell apoptosis was assayed by flow cytometry. The results showed that silencing AnxA2 gene by siRNA resulted in decreased expressions of AnxA2 gene and protein, increased apoptosis of U266 and RPMI8226 cell lines (P < 0.

High expression levels of COX-2 and P300 are associated with unfavorable survival in laryngeal squamous cell carcinoma.

In order to provide a basis for clinical treatment decisions, we explored whether there was a correlation between the expression of COX-2 and P300 and clinical factors in a group of patients with laryngeal squamous cell carcinoma (LSCC). A retrospective analysis of clinicopathological data was conducted in 80 patients with LSCC who presented between January 1997 and December 1998. An immunohistochemistry tissue microarray was conducted of 80 surgically resected LSCC and 20 adjacent normal tissue specimens.

Expression of phosphorylated mTOR and its regulatory protein is related to biological behaviors of ameloblastoma.

The present study investigated the relationship between the expression of p-mTOR, p-4EBP1 and p-p70S6K in the cytoplasm and nucleus of ameloblastoma (AB) cells and the invasiveness of ABs. Immunohistochemistry was performed to detect the expression of p-mTOR, p-4EBP1 and p-p70S6K in ABs and the level of these proteins in the nucleus and cytoplasm was scored. There was ectopic expression of p-mTOR in ABs.

GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer.

Background: Overexpression of GOLPH3 (Golgi phosphoprotein 3, 34 kDa) is associated with the progression of many solid tumor types leading to an unfavorable clinical outcome. We aimed to investigate the clinical significance of GOLPH3 expression in the development and progression of clinically N0 (cN0) oral tongue cancer.

Methods: Real-time PCR and Western blotting analyses were employed to examine GOLPH3 expression in four oral tongue cancer cell lines, primary cultured normal tongue epithelial cells (TEC), eight matched pairs of oral tongue cancer samples and adjacent noncancerous tissue samples from the same patient.

A review of functional magnetic resonance imaging for Brainnetome.

The functional brain network using blood-oxygen-level-dependent (BOLD) functional magnetic resonance imaging (fMRI) has revealed the potentials for probing brain architecture, as well as for identifying clinical biomarkers for brain diseases. In the general context of Brainnetome, this review focuses on the development of approaches for modeling and analyzing functional brain networks with BOLD fMRI. The prospects for these approaches are also discussed.

Decreased expression of SATB2: a novel independent prognostic marker of worse outcome in laryngeal carcinoma patients.

Background: To investigate the expression and role of special AT-rich sequence-binding protein-2 (SATB2) in laryngeal squamous cell carcinoma (LSCC) tissue and cell line (HEp2), and to evaluate the clinical and prognostic significance of SATB2 protein in patients with LSCC.

Methods: The expression of SATB2 was examined in LSCC tissue and HEp2 cells by Western-blotting, Real-time PCR and immunohistochemical staining. Cell growth curve assay and colony formation assay were used to verify the effect of SATB2 on the proliferation and tumor progression ability of HEp2 cells.