Publications by authors named "Ming Hai Hou"
Alternative splicing (AS) contributes to the complexity of the mammalian proteome and plays an important role in diseases, including infectious diseases. The differential AS patterns of these transcript sequences between the healthy (HS3A) and mastitic (HS8A) cows naturally infected by Staphylococcus aureus were compared to understand the molecular mechanisms underlying mastitis resistance and susceptibility. In this study, using the Illumina paired-end RNA sequencing method, 1352 differentially expressed genes (DEGs) with higher than twofold changes were found in the HS3A and HS8A mammary gland tissues.
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- - This study investigated the impact of single nucleotide polymorphisms (SNPs) in the promoter region of the INCENP gene on the quality of bovine semen in 250 Chinese Holstein bulls using the PCR-RFLP method.
- - Two SNPs (g.-556 G>T and g.-692 C>T) were identified, along with three haplotypes, and their frequencies were analyzed in relation to sperm motility, revealing that certain genotypes and haplotypes showed significantly better motility.
- - Functional experiments indicated that these SNPs are likely functional mutations, as the TG haplotype exhibited the highest activity in regulating the INCENP gene expression, suggesting a potential mechanism for how these genetic variations influence semen
Heat-shock transcription factors (HSFs) play an important role in regulating heat stress response. The activation of heat-shock protein (HSP) genes is mediated by HSFs, which bind to promoters of HSP genes. In this research, two novel single nucleotide polymorphisms, T909C and G4693T, and their association with thermal tolerance were investigated in 951 Chinese Holstein cattle.
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- Bovine lactoferrin (LF) is a glycoprotein in milk that helps protect the mammary gland, and recent research explored its genetic variations related to mastitis in dairy cows.
- The study genotyped specific mutations in the LF gene using 268 Chinese Holstein cows and employed statistical models to assess their impact on somatic cell scores (SCS), a measure of mastitis.
- Results showed significant associations between specific genotypes (-478/G and +72(T/C)) and lower SCS, indicating their potential as markers for selecting mastitis-resistant dairy cattle.
Complex vertebral malformation (CVM), a lethal autosomal recessive inherited defect in Holstein calves, was newly reported worldwide. The molecular cause of CVM was a substitution of guanine by thymine (G-->T) in a solute carrier family 35 member 3 gene (SLC35A3), encoding UDP-N-acetylglucosamine transporter. It was characterized by stillborn, abortion, and premature birth.
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