Enzymatic detergent in rat peritoneum. A comparative study with physiological solution.

Background: Peritoneal washing out with physiological solution with different substances added is useful in peritoneal infections, but the effect of enzymatic detergents, such as quaternary didecyl-dimethyl ammonium compounds (DDAC), used in the sterilization of surgical material is unknown. We undertook this study to determine histological changes (inflammation, fibrosis and new vessel formation) in the peritoneum of Wistar rats after the application of physiological solution or DDAC.

Methods: The minimum inhibitory concentration (MIC) of DDAC for E.

Molecular epidemiology of human cases of tuberculosis by Mycobacterium bovis in Mexico.

The purpose of this study was to evaluate the role of Mycobacterium bovis in human cases of tuberculosis (TB) in an endemic area of the disease in cattle. Sputum, urine and other tissue samples were obtained from: (1) TB-symptomatic patients, (2) dairy farm workers and (3) abattoir workers. Samples of macroscopic lesions suspicious of TB were also obtained from cattle at slaughter in the same geographic area.

[Risk factors for cognitive and functional impairment in the elderly].

Objective: To establish association between cognitive and functional impairment with economic, social and demographic factors, chronic disease and multidrug therapy in the aged patient.

Methods: A cross-sectional study was carried out in 2002 with 422 individuals 60 years old and older. A questionnaire for exploring social and economic conditions was applied.

[Molecular epidemiology of cattle and human tuberculosis in Mexico].

Objective: The purpose of the study was to determine the role of bovine TB in cases of human TB.

Material And Methods: Two-hundred and fifty-five samples from symptomatic patients were included in the study. All samples were cultured in Stonebrink and Lowënstein-Jensen media and analyzed using a nested PCRMPB70.