Evaluation of the overnight hold of whole blood at room temperature, before component processing: platelets (PLTs) from PLT-rich plasma.

Background: Whole blood (WB) must be refrigerated within 8 hours to optimize Factor VIII: C yield, but chilled platelets (PLTs) are rapidly removed from circulation and cannot be used clinically. It is logistically preferable to hold WB overnight at room temperature for next-day processing. We compared in vitro quality of PLT-rich plasma (PRP)-derived PLT concentrates (PCs) from fresh versus overnight-held WB.

A comparison of two robotic platforms to screen plateletpheresis donors for HLA antibodies as part of a transfusion-related acute lung injury mitigation strategy.

Background: Efforts to minimize white blood cell alloantibodies, responsible for transfusion-related acute lung injury (TRALI) in components with high-volume single-donor plasma include consideration of plateletpheresis donor screening for human leukocyte antigen (HLA) antibodies. High-throughput screening platforms make this feasible for large blood centers. Which platform to use, donor subgroups to screen, characteristics of detected antibodies, and operational impact of deferring reactive donors are important questions.

Maintenance of in vitro properties of leukoreduced whole blood-derived pooled platelets after a 24-hour interruption of agitation.

Background: Continuous agitation during platelet concentrate (PC) storage is frequently interrupted during shipping. Studies have evaluated the effects of interrupted agitation in apheresis and single whole blood-derived PCs, but not PC pools. This study evaluated in vitro properties of pooled whole blood-derived platelets (PLTs) after a 24-hour interruption of agitation.

Comparison of the in vitro properties of apheresis platelets during 7-day storage after interrupting agitation for one or three periods.

Background: Many platelet (PLT) components undergo multiple periods of shipment before transfusion. We have previously conducted studies investigating maintenance of apheresis PLT in vitro quality measures during a single 24- or 30-hour interruption of agitation, but data are not available for multiple periods without agitation.

Study Design And Methods: Apheresis PLTs were collected with both the Amicus (Fenwal, Inc.

The influence of simulated shipping conditions (24- or 30-hr interruption of agitation) on the in vitro properties of apheresis platelets during 7-day storage.

Background: Platelet (PLT) components undergo interruption of agitation during shipment. Studies have demonstrated maintenance of PLT quality of whole blood-derived PLT concentrates during a 24-hour interruption of agitation, but data are not available for apheresis PLTs in 100 percent plasma.

Study Design And Methods: Apheresis PLTs were collected with one of two commercially available separators (Amicus, Fenwal, Inc.