Isoelectric focusing of serum alkaline phosphatase isoenzymes.

We present a new method for the separation of alkaline phosphatase isoenzymes based on isoelectric focusing of serum proteins. Sera of healthy subjects studied by this method present seven groups of bands within a pH range of 3-9. To date, the organ source of six out of seven groups has been identified directly or indirectly.

Quality of breast milk: its control and preservation.

The numerous problems of bacterial contamination and proper preservation of breast milk have been examined. Both the many advantages of human milk as the optimal nourishment for the newborn baby and the extensive experiences of veterinary medicine in the handling of milk have been carefully considered. Bacterial multiplication has been studied under various storage conditions.

Bilateral occurrence of pheochromocytoma in patients with the multiple endocrine neoplasia syndrome type 2A (Sipple's syndrome).

Two kindreds with the multiple endocrine neoplasia type 2A syndrome were studied. Of one of these we examined 150 members, 20 of whom were treated with thyroidectomy for medullary carcinoma and nine with bilateral adrenalectomy for pheochromocytoma. Of the second kindred 50 members were examined, seven of whom were thyroidectomized and seven treated with bilateral adrenalectomy.

[Determination of urea in blood and serum by the reflotest-urea method (author's transl)].

Tests in five laboratories on pooled sera in three concentration ranges demonstrated good reproducibility of urea concentration with the Reflotest-Urea, at a variation coefficient from 2.4% to 5.0%.

[Determination of thyroxine in serum by a heterogeneous enzyme immunoassay: results of a joint trial].

This paper describes the evaluation of a heterologous enzyme immunoassay for the determination of total thyroxine in serum by a group of seven clinical chemical laboratories. The test follows the principles of the enzyme linked immunosorbent assay (ELISA) and uses peroxidase as a marker. The evaluation of analytical reliability yielded the following results within the analytical range from 39 unto 322 nmol/l: 1.