Detection of Gram-positive and Gram-negative bacteria membrane permeabilization induced by pulsed electric field using electrochemical admittance spectroscopy.

Electrochemical impedance or admittance spectroscopy (EIS or EAS) has been widely used for decades, offering a label-free, rapid, real-time, and non-destructive assay for optically opaque and turbid bacterial solutions. However, PEF-induced changes in the bacterial envelope can present challenges in detecting the extent of membrane permeabilization in both Gram-positive and Gram-negative bacteria due to their distinct morphological properties. Here, we used a new approach for detecting bacterial membrane permeabilization induced by PEF using electrochemical admittance spectroscopy (EAS).