Preparation of highly sensitive protein array using reactive polymer.

We have discovered a novel protein immobilization method, i.e., a "Three-Dimensional Nanostructured Protein Hydrogel" (3-D NPH), which is composed of protein-reactive polymer hybrid nanoparticles to detect protein-protein interactions.

Enhancement of sensitivity of SPR protein microarray using a novel 3D protein immobilization.

We discovered a novel method to prepare a protein-based hydrogel, that is, a "Three-Dimensional Nanostructured Protein Hydrogel (3D NPH)", which is composed of protein-polymer hybrid nanoparticles. In this study, we propose a novel protein microarray whose 3D NPH spots were prepared by dispensing a small volume of the solution of protein-polymer mixture on a substrate. The dispensed solution had a short time for cross-linking before its drying-up and the resulting 3D NPH had loosely cross-linked, thin spongy structure.