KAI1 is a potential target for anti-metastasis in pancreatic cancer cells.

Aim: To investigate whether KAI1, as a metastasis suppressor gene, is associated with invasive and metastatic ability of pancreatic cancer cells.

Methods: KAI1 gene was transfected into pancreatic cancer cell line MiaPaCa II by liposomes selected with G418. Expression of transfected cells was measured by Western blotting, immunofluorescence and immunocytochemistry.

New tumor-associated antigen SC6 in pancreatic cancer.

Aim: To examine the concentration of a new antigen SC6 (SC6-Ag) recognized by monoclonal antibody (MAb) in patients with pancreatic cancer and other malignant or benign diseases and to understand whether SC6-Ag has any clinical significance in distinguishing pancreatic cancer from other gastrointestinal diseases.

Methods: Six hundred and ninety-five serum specimens obtained from 115 patients with pancreatic cancer, 154 patients with digestive cancer and 95 patients with non-digestive cancer were used and classified in this study. Serum specimens obtained from 140 patients with benign digestive disease and 89 patients with non-benign digestive disease served as controls.

KAI1 inhibits anchorage-dependent and -independent pancreatic cancer cell growth.

Decreased expression of the tumor suppressor gene, KAI1, is associated with metastasis formation in pancreatic cancer. The aim of the present study was to investigate whether KAI1 influences pancreatic cancer cell growth and colony formation. A full-length KAI1 cDNA expression vector was stably transfected into Panc-1 and MiaPaCa-2 pancreatic cancer cell lines.

[The mechanism of KAI1 gene in inhibition of metastasis of primary pancreatic cancer].

Objective: To study the effect on invasive and motor ability of MiaPaCa II pancreatic cancer cell transfected by recombinant plasmid pCMV-KAI1, alteration of KAI1 in the transfecting cancer cells, and the metastatic mechanism in the malignancy.

Methods: MiaPaCa II pancreatic cancer cell was transfected with pCMV-KAI1 by liposome, the invasive and gelatinase ability of two pancreatic cancer cell lines were analyzed with transwell culture cell and polyacrylamided gel electrophoresis (PAGE) before and after KAI1 cDNA transfection.

Results: After KAI1 cDNA transfected into MiaPaCa II, the migrative ability of passing through the membrane filter decreased evidently (P < 0.

Effect of bax, bcl-2 and bcl-xL on regulating apoptosis in tissues of normal liver and hepatocellular carcinoma.

Aim: To investigate the expression of bax, bcl-2 and bcl-xL mRNA in the tissues of normal liver and hepatocellular carcinoma (HCC), and analyze the relationship between the expression of bax, bcl-2 and bcl-xL mRNA and clinical parameters of HCC patients.

Methods: The expression of bax, bcl-2 and bcl-xL mRNA of normal liver and HCC was measured by Northern blot. Statistical analyses were made by t test and correlation analysis.

KAI1 gene is differently expressed in papillary and pancreatic cancer: influence on metastasis.

AIM:To compare KAI1 in cancer of papilla of Vater and pancreas to evaluate whether there are differences in biologic behavior which might account for prognosis.METHODS:We compared the expression in 24 papillay and 29 pancreatic cancers using Northern blot analysis, immunochemical assay and in situ hybridization, and investigated whether early diagnosis or molecular differences predict the outcome in these tumor entities.RESULTS:By Northern blot analysis there is no statistical difference of KAI1 levels in normal and cancerous papilla.