Identification and characterization of a novel antioxidant peptide from feather keratin hydrolysate.

Objectives: To improve the potential value of feather, which is a valuable protein resource, we have separated and identified antioxidant peptide(s) from feather hydrolysate.

Results: Feather hydrolysate was prepared by fermentation with Bacillus subtilis S1-4. Antioxidative peptides were separated by sequential acid precipitation, cation exchange, and reversed-phase fast performance liquid chromatography.

Substrate specificity and thermostability of the dehairing alkaline protease from Bacillus pumilus.

An alkaline protease (DHAP) from Bacillus pumilus has shown great potential in hide dehairing. To get better insights on its catalytic properties for application, the substrate specificity and thermostability were investigated using five natural proteins and nine synthetic peptides. The results showed that DHAP could hydrolyze five proteins tested here in different specificity.