Cytosensing and evaluation of cell surface glycoprotein based on a biocompatible poly(diallydimethylammonium) doped poly(dimethylsiloxane) film.

In this paper, we constructed an interface that not only retains viability of immobilized BGC823 human gastric carcinoma cells (BGC823 cells) but also efficiently resists nonspecific adsorption of the P-glycoprotein antibody and its secondary antibody, which enabled us to sensitively detect the number of cells and P-glycoproteins on the BGC823 cell surface by the immunoassay method. Preparation of the film was quite simple and inexpensive just by spin-coating poly(dimethylsiloxane) (PDMS) doped with poly(diallydimethylammonium) (PDDA) on the surface of gold electrodes. The composite film's biocompatibility, antinonspecific adsorption ability, and the conductivity for electrochemical probe ([Fe(CN)6]3-/4-) were proved by cell culture experiments, blocking experiments, and electrochemical experiments.

Patterned Au/poly(dimethylsiloxane) substrate fabricated by chemical plating coupled with electrochemical etching for cell patterning.

In this paper, we present a novel approach for preparing patterned Au/poly(dimethylsiloxane) (PDMS) substrate. Chemical gold plating instead of conventional metal evaporation or sputtering was introduced to achieve a homogeneous gold layer on native PDMS for the first time, which possesses low-cost and simple operation. An electrochemical oxidation reaction accompanied by the coordination of gold and chloride anion was then exploited to etch gold across the region covered by electrolyte.