Roles of Carbohydrate-Binding Module (CBM) of an Endo-β-1,4-Glucanase (Cel5L) from sp. KD1014 in Thermostability and Small-Substrate Hydrolyzing Activity.

An endo-β-1,4-glucanase gene, 5L, was cloned using the shot-gun method from sp.. The gene, which contained a predicted signal peptide, encoded a protein of 496 amino acid residues, and the molecular mass of the mature Cel5L was estimated to be 51.