Versatile, do-it-yourself, low-cost spinning disk confocal microscope.

Confocal microscopy is an invaluable tool for 3D imaging of biological specimens, however, accessibility is often limited to core facilities due to the high cost of the hardware. We describe an inexpensive do-it-yourself (DIY) spinning disk confocal microscope (SDCM) module based on a commercially fabricated chromium photomask that can be added on to a laser-illuminated epifluorescence microscope. The SDCM achieves strong performance across a wide wavelength range (∼400-800 nm) as demonstrated through a series of biological imaging applications that include conventional microscopy (immunofluorescence, small-molecule stains, and fluorescence in situ hybridization) and super-resolution microscopy (single-molecule localization microscopy and expansion microscopy).

Fluorescent labeling of abundant reactive entities (FLARE) for cleared-tissue and super-resolution microscopy.

Fluorescence microscopy is a vital tool in biomedical research but faces considerable challenges in achieving uniform or bright labeling. For instance, fluorescent proteins are limited to model organisms, and antibody conjugates can be inconsistent and difficult to use with thick specimens. To partly address these challenges, we developed a labeling protocol that can rapidly visualize many well-contrasted key features and landmarks on biological specimens in both thin and thick tissues or cultured cells.

Incorporation of sensing modalities into de novo designed fluorescence-activating proteins.

Through the efforts of many groups, a wide range of fluorescent protein reporters and sensors based on green fluorescent protein and its relatives have been engineered in recent years. Here we explore the incorporation of sensing modalities into de novo designed fluorescence-activating proteins, called mini-fluorescence-activating proteins (mFAPs), that bind and stabilize the fluorescent cis-planar state of the fluorogenic compound DFHBI. We show through further design that the fluorescence intensity and specificity of mFAPs for different chromophores can be tuned, and the fluorescence made sensitive to pH and Ca for real-time fluorescence reporting.

Robust State/Fault Estimation and Fault-Tolerant Control in Discrete-Time T-S Fuzzy Systems: An Embedded Smoothing Signal Model Approach.

This study investigates a simple design method of the robust state/fault estimation and fault-tolerant control (FTC) of discrete-time Takagi-Sugeno (T-S) fuzzy systems. To avoid the corruption of the fault signal on state estimation, a novel smoothing signal model of fault signal is embedded in the T-S fuzzy model for the robust H state/fault estimation of the discrete-time nonlinear system with external disturbance by the traditional fuzzy observer. When the component and sensor faults are generated from different fault sources, two smoothing signal models for component and sensor faults are both embedded in the T-S fuzzy system for robust state/fault estimation.

Multiplayer Noncooperative and Cooperative Minimax H Tracking Game Strategies for Linear Mean-Field Stochastic Systems With Applications to Cyber-Social Systems.

The multiplayer stochastic noncooperative tracking game (NTG) with conflicting target strategy and cooperative tracking game (CTG) with a common target strategy of the mean-field stochastic jump-diffusion (MFSJD) system with external disturbance is investigated in this study. Due to the mean (collective) behavior in the system dynamic and cost function, the designs of the NTG strategy and CTG strategy for target tracking of the MFSJD system are more difficult than the conventional stochastic system. By the proposed indirect method, the NTG and CTG strategy design problems are transformed into linear matrix inequalities (LMIs)-constrained multiobjective optimization problem (MOP) and LMIs-constrained single-objective optimization problem (SOP), respectively.

Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy.

Fluorescence microscopy is a workhorse tool in biomedical imaging but often poses substantial challenges to practitioners in achieving bright or uniform labeling. In addition, while antibodies are effective specific labels, their reproducibility is often inconsistent, and they are difficult to use when staining thick specimens. We report the use of conventional, commercially available fluorescent dyes for rapid and intense covalent labeling of proteins and carbohydrates in super-resolution (expansion) microscopy and cleared tissue microscopy.

Stochastic Robust Team Tracking Control of Multi-UAV Networked System Under Wiener and Poisson Random Fluctuations.

In this article, a robust leader-follower tracking control scheme is proposed to deal with the stochastic multi-unmanned aerial vehicle (UAV) networked team tracking control problem to achieve a prescribed H robust tracking performance. By taking the leader's desired path and every UAV networked system into account, the leader-follower tracking error networked system is constructed by arranging the UAVs network systems into a leader-follower formation. To effectively reduce the effect of the external disturbance on the team tracking process, a robust H controller is proposed.

De novo design of a fluorescence-activating β-barrel.

The regular arrangements of β-strands around a central axis in β-barrels and of α-helices in coiled coils contrast with the irregular tertiary structures of most globular proteins, and have fascinated structural biologists since they were first discovered. Simple parametric models have been used to design a wide range of α-helical coiled-coil structures, but to date there has been no success with β-barrels. Here we show that accurate de novo design of β-barrels requires considerable symmetry-breaking to achieve continuous hydrogen-bond connectivity and eliminate backbone strain.

A novel molecular rotor facilitates detection of p53-DNA interactions using the Fluorescent Intercalator Displacement Assay.

We have investigated the use of fluorescent molecular rotors as probes for detection of p53 binding to DNA. These are a class of fluorophores that undergo twisted intramolecular charge transfer (TICT). They are non-fluorescent in a freely rotating conformation and experience a fluorescence increase when restricted in the planar conformation.

Molecular rotors as conditionally fluorescent labels for rapid detection of biomolecular interactions.

We demonstrate the use of fluorescent molecular rotors as probes for detecting biomolecular interactions, specifically peptide-protein interactions. Molecular rotors undergo twisted intramolecular charge transfer upon irradiation, relax via the nonradiative torsional relaxation pathway, and have been typically used as viscosity probes. Their utility as a tool for detecting specific biomolecular interactions has not been explored.

Antihyperlipidemic and antioxidant effects of extracts from Pleurotus citrinopileatus.

Pleurotus citrinopileatus is a popular edible mushroom which is physiologically active in both humans and animals. In the study we investigate the effects of this mushroom on hyperlipidemic hamster rats. Four dietary forms of the mushroom were created as follows.

Antihyperglycemic effect of polysaccharide from fermented broth of Pleurotus citrinopileatus.

Pleurotus citrinopileatus is an edible mushroom, which has recently become very popular, with a consequent increase in industrial production. Water-soluble polysaccharides (WSPS), extracted from edible mushrooms, have been found to have antitumor and immunoenhancing effects. In this study, we investigate the effects of WSPS extracted from submerged fermented medium of P.