Sequencing the genome of Marssonina brunnea reveals fungus-poplar co-evolution.

Background: The fungus Marssonina brunnea is a causal pathogen of Marssonina leaf spot that devastates poplar plantations by defoliating susceptible trees before normal fall leaf drop.

Results: We sequence the genome of M. brunnea with a size of 52 Mb assembled into 89 scaffolds, representing the first sequenced Dermateaceae genome.

Analysis of floral transcription factors from Lycoris longituba.

Transcription factors (TFs) are proteins that bind to specific promoter regions of their target genes and regulate gene transcription. Many of these factors have been found to influence flowering. Lycoris longituba exhibits a great deal of diversity in flower color and flower form, making it a suitable model for the study of floral development.

[The localization of galanthamine in vegetative organ of Lycoris aurea Herb].

Using laser confocal microscopical techniques, we observed the anatomical structure of mature root, bulb, and leaf of Lycoris aurea Herb., and also did some research on the localization of galanthamine in the above-mentioned vegetative organ. The results are as follows: In the mature root, the galanthamine distributes mainly in cell wall, especially in cell wall of exodermis and endodermis and vessel wall.

[Progress in research on forest tree comparative genomics].

Comparative genomics is one of the important research fields in genomics. By comparative genomic studies it was possible to establish a giant genetic system beyond one species which might be significant to tree. Comparative genomics in Salicaceae, Pinaceae, Rosaceae, Fagaceae and Hamamelidaceae had shown that the organization of genomes remained highly conserved over long evolutionary periods and extensive synteny or collinarity or microcollinarity existed in tree genomes.

[The analysis of differential expression and cloning of genes related to raised secondary lateral veins mutant of Lycoris aurea].

Lycoris aurea exhibits parallel venation, the main vein with many lateral veins in a longitudinal parallel arrangement. There are secondary lateral veins (SLV) between each longitudinal veins. In general, SLVs are not remarkable.

[Identification of markers linked to resistance locus of Marssonina leaf spot in poplars by bulked segregant analysis(BSA)].

DNA markers linked to resistance locus of Marssonina leaf spot in poplars were found by bulked segregant analysis(BSA). The bulks consisted of individual with a extreme phenotype taken from a population of 91 F1 clones,which is a progeny of Populus deltoides Bartr.cv.

[Analysis of genetic structure in population of Larix kaempferi by chloroplast SSR markers].

Genetic structure of seven populations in Larix kaempferi in Japan was studied by use of cpSSR markers. Ten different length fragments in and ten different kinds of haplotypes were reduced in 197 samples based on 3 pairs of polymorphic primers screened from 11 pairs of primers. There were significant variant haplotypes among the populations.

[Phylogeny of the genus Arundinaria based on nucleotide sequences of nrDNA ITS region].

The sequences of nrDNA regions of 17 species and Phyllostachys edulis (outgroup) sampled, which are of represent and type species for different taxa in the genus Arundinaria,were analyzed by PCR amplification and direct DNA sequencing. The phylogenetic trees generated from maximum parsimony analysis showed that the sampled bamboos were naturally monophletic, appearing that these species of the bamboos belong to the genus Arundinaria. The internal transcribed spacers (ITS) data indicated that the species were divided into two branches, one including A.

[Analysis of the inter-species relationships on lycoris Amaryllidaceae) by use of RAPD].

The fingerprints of 13 species in genus Lycoris were generated by use of RAPD method. Forty-one primers were screened from 520 random primers, and a total of 350 DNA fragments were amplified ranging from 0.3-3.

[Sequence of the ITS region of nuclear ribosomal DNA(nrDNA) in Xinjiang wild Dianthus and its phylogenetic relationship].

Xinjiang is a center of distribution and differentiation of genus Dianthus in China, and has a great deal of species resources. The sequences of ITS region (including ITS-1, 5.8S rDNA and ITS-2) of nuclear ribosomal DNA from 8 species of genus Dianthus wildly distributed in Xinjiang were determined by direct sequencing of PCR products.