Risk factors for complications of tissue expansion: An updated systematic review and meta-analysis.

Background: This study aimed to summarize risk factors for complications of tissue expansion using a meta-analysis of cohort studies.

Methods: PubMed, Embase, and Cochrane Library were searched from January 1985 to January 2025 for retrospective cohort studies investigating at least one potential risk factor for complications of tissue expansion. The quality of individual studies was assessed using the Newcastle-Ottawa scale.

An analysis of the burden of respiratory tract cancers in global, China, the United States and India: findings based on the GBD 2021 database.

Objective: This study aimed to assess the burden of respiratory tract cancers in China, the United States, India, and worldwide from 1990 to 2021. Also, forecast the evolution of respiratory tract cancers deaths and DALYs burden during 2022 to 2050.

Study Design: An epidemiological analysis.

Analysis of Codon Usage Patterns in a Rare Palmitoleic Acid Production Oleaginous Yeast, Scheffersomyces segobiensis.

Scheffersomyces segobiensis DSM27193 has been identified as a potential host for Palmitoleic acid (POA)-rich lipids production. Our previous study has found that S. segobiensis DSM27193 possesses a broad substrate spectrum, excellent xylose fermentation capability, rapid growth, high biomass, and an abundance of intracellular acetyl-CoA.

Genomic epidemiology and antimicrobial resistance reveal local transmission dynamics of enteric fever in Shenzhen, one of the mega cities in China.

Objectives: Outbreak of enteric fever and the spread of antimicrobial-resistant Salmonella Typhi and S. Paratyphi pose significant public health challenges in low- and middle-income countries. Understanding the transmission dynamics of these pathogens is essential for developing effective control strategies.

From Function to Mechanism: Unveiling the Role of Small Nucleolar Ribonucleic Acids in Digestive Tumours.

Small nucleolar ribonucleic acids (snoRNAs) have emerged as crucial regulators in various biological processes and have garnered significant attention for their potential roles in cancer. These noncoding ribonucleic acids (RNAs) primarily guide ribosomal RNA (rRNA) pseudouridylation and 2'-O-methylation modifications and exhibit stable expression in the serum, making them promising biomarkers and therapeutic targets. Digestive tract cancer poses a severe global health threat due to its high mortality rate and difficulty in early detection.

Unsupervised Learning of Intrinsic Semantics with Diffusion Model for Person Re-Identification.

Unsupervised person re-identification (Re-ID) aims to learn semantic representations for person retrieval without using identity labels. Most existing methods generate fine-grained patch features to reduce noise in global feature clustering. However, these methods often compromise the discriminative semantic structure and overlook the semantic consistency between the patch and global features.

An Efficient Dynamic Resource Allocation Framework for Evolutionary Bilevel Optimization.

Bilevel optimization problems (BLOPs) are characterized by an interactive hierarchical structure, where the upper level seeks to optimize its strategy while simultaneously considering the response of the lower level. Evolutionary algorithms are commonly used to solve complex bilevel problems in practical scenarios, but they face significant resource consumption challenges due to the nested structure imposed by the implicit lower-level optimality condition. This challenge becomes even more pronounced as problem dimensions increase.

The value of plasma sCD25 in diagnosis, therapeutic efficacy, and prognosis of acute myeloid leukemia.

This study aims to investigate the clinical importance of soluble CD25 (sCD25) levels in diagnosing acute myeloid leukemia (AML), predicting patient outcomes, and monitoring treatment responses. Plasma sCD25 levels were measured in 190 AML patients and 47 healthy controls. AML patients were further divided into subgroups based on chemotherapy status, therapeutic response, and prognostic risk.

Utilizing network pharmacology and experimental validation to explore the mechanisms of the Qijiafuzheng formula promoting CD8+ T-cell infiltration in the microenvironment of lung adenocarcinoma through STAT1/CXCL10.

Qijiafuzheng formula (QJFZF), a Traditional Chinese Medicine used to treat lung cancer and mitigate chemotherapy side effects, was studied to clarify its impact on the tumor immune microenvironment (TIME). Using network pharmacology and experimental validation, 39 overlapping targets were identified from 579 QJFZF-related and 752 lung adenocarcinoma (LUAD)-TIME targets. Key genes (CCL3, IL10, CXCL10, FOXP3, CD86) correlated negatively with tumor purity and positively with CD8+ T-cell infiltration.

Ambient air pollution does not diminish the beneficial effects of active commuting on heart failure: a prospective study in UK Biobank.

Background: Evidence on active commuting and heart failure (HF) under levels of air pollution exposure is limited, and little is known on the potential mediation roles of inflammation. The objective of this study was to investigate the association between active commuting and HF, as well as to ascertain whether air pollution may influence this relationship.

Methods: This prospective study included 241,786 participants without HF at baseline in UK Biobank.

Exploring biotechnology for plastic recycling, degradation and upcycling for a sustainable future.

The persistent demand for plastic commodities, inadequate recycling infrastructure, and pervasive environmental contamination due to plastic waste present a formidable global challenge. Recycling, degradation and upcycling are the three most important ways to solve the problem of plastic pollution. Sequential enzymatic and microbial degradation of mechanically and chemically pre-treated plastic waste can be orchestrated, followed by microbial conversion into value-added chemicals and polymers through mixed culture systems.

Unraveling the evolution of the ATB2 subgroup basic leucine zipper transcription factors in plants and decoding the positive effects of BdibZIP44 and BdibZIP53 on heat stress in Brachypodium distachyon.

In plants, basic region/leucine zipper motif (bZIP) transcription factors (TFs) stand as pivotal regulators in a broad spectrum of developmental mechanisms and adaptive strategies against environmental pressures. However, the ancestral origins and the evolutionary progression of their functional diversity across plant species have yet to be thoroughly illuminated. This study delved into the ATB2 subgroup bZIP homologs, tracing them back to the ancestral charophyte lineage predating land plant emergence, and categorized them into four distinct phylogenetic clusters (Clades A to D).

Folium Hibisci Mutabilis extract suppresses M1 macrophage polarization through mitochondrial function enhancement in murine acute gouty arthritis.

Background: Acute gout arthritis (AGA) is a common metabolic joint disease and urgently needs a safer alternative therapy due to the significant side effects from long-term use of primary medications. Folium Hibisci Mutabilis, a traditional medicinal herb, has demonstrated promising therapeutic efficacy in the clinical management of AGA, but its pharmacological mechanisms remain to be elucidated.

Methods: Folium Hibisci Mutabili was isolated and refined into the Folium Hibisci Mutabilis Extract (FHME).

Introgression of in maize hybrids enhances resistance to gray leaf spot disease without compromising yield.

Unlabelled: Gray leaf spot (GLS) is one of the most damaging foliar diseases in maize. In previous research, we identified the gene, which confers resistance to GLS. This study demonstrates the utility of in breeding resistant maize varieties.

Ligustilide covalently binds to Cys254 of the creatine kinase, M-type protein, ameliorating acute myocardial ischemia by enhancing the creatine phosphate level.

Background: Myocardial ischemia (MI) threatens the health of middle-aged and older adults by reducing cardiac oxygen supply and function. Current therapies, including vasodilation, thrombolysis, and interventions, focus on relieving symptoms and improving blood flow but do not adequately address underlying energy metabolism issues. Ligustilide exerts a protective effect on the cardiovascular system and holds the potential for ameliorating MI; however, there is currently no systematic elucidation of ligustilide's target and action mechanism for MI.

Exploration of Regulation of Alcohol Dehydrogenation Reaction of Dibenzimidazole-Based Ruthenium Complexes.

This work demonstrates the synthesis of a series of Ru(II) complexes with dibenzimidazole-based ligands and investigates the spatial relationship between ligand structure and complexes. It explores the effect of substituent changes on the N-Ru-N bond angle of the complexes, as well as the distance from the Ru center to the aryl group, and utilizes these complexes to catalyze benzyl alcohol dehydrogenation in toluene. It was found that the ligand [Ru]-complex with a spatial configuration of electron donating groups had a larger N-Ru-N bond angle and higher catalytic performance at the ruthenium center, with a yield of 91.

Harnessing prazosin for tumors: Liposome hybrid nanovesicles activate tumor immunotherapy via autophagy inhibition.

Prazosin (Prz), an antagonist of alpha-1 adrenergic receptors, is conventionally employed in the treatment of hypertension. Our study pioneers the exploration of Prz in oncology, examining its impact on cellular autophagy and its potential to trigger antitumor immune responses. We have developed a novel Prz-loaded liposome hybrid nanovesicle (Prz@LINV) system, integrating tumor-derived nanovesicles (TNV) with liposomes (LIP) to facilitate targeted Prz delivery to tumor sites.

Amino-β-cyclodextrin derivatives with different chain lengths as chiral selectors for separation of dansyl amino acid enantiomers by capillary electrophoresis.

To date, cyclodextrins (CDs) and their derivatives are recognized as highly effective chiral selectors in electrophoresis for enantiomer separation due to their numerous advantages. In this study, three amino-β-CD derivatives, including NH-β-CD-5 (Mono-(6-(tetraethylenepentamine)-6-deoxy)-beta-Cyclodextrin), NH-β-CD-3 (mono-(6-(diethylenetriamine)-6-deoxy)-β-Cyclodextrin), and NH-β-CD-1 (6-Monodeoxy-6-monoamino-beta-cyclodextrine) with varying amino substituent chain lengths were employed as chiral selectors in capillary electrophoresis (CE) to examine their chiral selectivity in the separation of five dansylated amino acid enantiomers, namely Dns-DL-Val, Dns-DL-Leu, Dns-DL-Thr, Dns-DL-Phe, and Dns-DL-Ser, as the chain length of the substituent group significantly impacts separation performance. Among the three amino-β-CD derivatives, NH-β-CD-5 exhibited superior performance by separating four dansylated amino acid enantiomers, whereas NH-β-CD-3 and NH-β-CD-1 separated only two and one enantiomers, respectively.

Integrated physiological and transcriptomic data revealed the cold-resistant mechanisms in reproductive organs of the 'Jinguang' pear cultivar.

The spp. (pears) are crucial for the fruit industry; however, low spring temperatures can cause frost damage to their reproductive organs, which poses challenges to the final yields. In this study, we evaluated the response of the flowers and young fruits of the 'Jinguang' pear cultivar to low temperatures from integrated phenotypic, physiological, and molecular approaches.

Effectiveness and Safety of Oral Compound Glycyrrhizin Followed by Phototherapy for the Treatment of Progressive Vitiligo in Children.

Childhood vitiligo, distinct from its adult counterpart, presents unique treatment challenges. Glycyrrhizin inhibits the release of high-mobility group box 1 (HMGB1) protein from keratinocytes, preventing melanocyte apoptosis and autophagy. Furthermore, the orally administered compound glycyrrhizin (OCG) effectively treats various autoimmune disorders, demonstrating long-term efficacy, safety, and tolerability.

Application of 2D polymerase chain reaction for single-tube detection of high-risk human papillomaviruses.

Purpose: The persistent infection of high-risk HPV (HR-HPV) is intricately linked to the onset and progression of cervical cancer. This research endeavored to develop a high-throughput 2D PCR method for closed-tube genotyping of 11 HR-HPVs.

Methods: Base-quenched probes were specifically designed for FAM, VIC, and CY5 channels.

Development of Recombinant Human Collagen-Based Porous Scaffolds for Skin Tissue Engineering: Enhanced Mechanical Strength and Biocompatibility.

Skin tissue engineering scaffolds should possess key properties such as porosity, degradability, durability, and biocompatibility to effectively facilitate skin cell adhesion and growth. In this study, recombinant human collagen (RHC) was used to fabricate porous scaffolds via freeze-drying, offering an alternative to animal-derived collagen where bovine collagen (BC)-based scaffolds were also prepared for comparison. The internal morphology of the RHC scaffolds were characterized by scanning electron microscopy (SEM) and the pore size ranged from 68.

The Role and Challenges of Investigator-Initiated Trials in the Cell and Gene Therapy Products Boom in Mainland China.

As cutting-edge technologies in biomedicine, cell and gene therapy (CGT) products demonstrate immense potential in treating cancer, rare diseases, and genetic disorders, thereby driving the importance of clinical research in this area. This study analyzes the growth trends and key characteristics of 1033 Investigator-Initiated Trials (IITs) conducted by mainland Chinese institutions in the CGT field. The results show that IITs have played a positive role in the early proof-of-concept of CGT products, helping to obtain preliminary safety and efficacy data, and exploring the combination of CGT products with other therapies.

High Ectoine Production from Lignocellulosic Hydrolysate by through Metabolic and Fermentation Engineering.

Ectoine, a major compatible solute in halophilic micro-organisms, shows great potential in cosmetics and pharmaceuticals areas owing to its water-binding properties and capability to prevent oxidative damage. In this study, the gene cluster responsible for the ectoine synthesis originated from halophilic bacterium was first assembled into . Subsequently, the gene in PTS was knocked out to further drive the metabolic flux from phosphoenolpyruvate to oxaloacetate, resulting in 1.

Separation and Purification of Ginsenosides and Flavonoids in From the Leaves and Stems of Panax quinquefolium by High-Speed Countercurrent Chromatography and Online-Storage Inner-Recycling Countercurrent Chromatography.

Study aimed to isolate and purify compounds from the stems and leaves of Panax quinquefolius. By employing a highly innovative separation technique that combined multistage countercurrent chromatography (MRCC), high-speed countercurrent chromatography (HSCCC), and an advanced online-storage inner-recycling countercurrent chromatography (OS-IRCCC) mode for the first time, 12 compounds were successfully isolated, including 10 ginsenosides and 2 flavonoids. First, the crude extract was fractionated into five parts using D101 MRCC, with HPLC analysis revealing that 40% and 60% ethanol eluate contained the highest compound diversity.