Oxygen-binding Properties of Complexes between Dextran and Pyridoxal 5-Phosphate-Modified Porcine Hemoglobin.

It was demonstrated by oxygen equilibrium curve that the pyridoxal 5-phosphate(PLP) modified porcine deoxyhemoglobin(pHbbeta) had lower oxygen affinity than that of stroma-free porcine hemoglobin(pHb). Accourding to analysis of SDS-PAGE, the porcine hemoglobin derivatives were not cross-linked between its subunits. A conjugate was synthesized between pHbbeta and p-toluenesolfonyl chloride-actived dextran.

Preparation of Inositol Tetrakisphosphate and Its Application in Modification of Porcine Hemoglobin.

Modification of hemoglobin using inositol tetrakisphosphate (IP(4)) can improve the oxygen affinity of hemoglubin. Phytase was extracted from wheat bran and purified by ammonium sulphate fractionation, followed by Sephadex G-50 gel filtration and Mono Q chromatography. The purified phytase was used for hydrolysis of phytic acid under controled conditions.

PEG and DBBF modified porcine hemoglobin and their oxygen-carrying capacity.

Modifications of proteins with polyethylene glycol (PEG) have been proven to enlarge molecule size of proteins, to prolong their retention time in the circulation as well as blunt immune or allergic reactions. Hemoglobin cross-linked with small molecular modifiers turns out to be more stable and to have better oxygen carrying capacity. In the present study, four derivatives of PEG with different activation groups, and several PEGs with different molecular weights were covalently bound to porcine hemoglobin (pHb).

Phenylethanolamine N-methyl transferase expression in mouse thymus and spleen.

Catecholamines usually are found in neurons and chromaffin cells of mammals. In this study, surprisingly high levels of the epinephrine synthesizing enzyme phenylethanolamine N-methyl transferase (PNMT) were detected in the thymus of young mice. Levels of PNMT activity in the thymus were comparable to levels in the brainstem and were suppressed by the PNMT inhibitor LY134046.

[A case of human thelaziasis occurred in both eyes].

On October 26, 1987, the authors encountered a case of human thelaziasis involving both eyes. The case in Chollapuk-do, Korea, a farmer aged 23, complained of mild lacrimation for 3 weeks followed by 1 week duration of foreign body sensation in both eyes prior to examination of eyes. The conjunctivae of both eyes appeared slightly reddened.

[Effect of aflatoxin B1 on ultrastructural changes of biliary epithelial cells in mice experimentally infected with Clonorchis sinensis].

The present study was carried out to examine the effect of a carcinogen, aflatoxin B1 on the ultrastructural changes of biliary epithelial cells in mice infected with Clonorchis sinensis. A total of 93 male albino mice(BALB/c strain) was divided into 3 groups; group I, treated with 1.0 ppm aflatoxin B1 for 12 weeks; group II, given 50 C.

[An Epidemiological Study On Zoonoses In Korea].

Helminthic zoonoses are of significant importance to the public health and to the socioeconomic consequences especially in lost-meat, animal products and animal labor. For past several decades in Korea, it has been recognized that endoparasitic infections among domestic animals are extremely common and many of the helminth parasites of man have been acquired from, or are shared with domestic animals. However, there was no survey of a nationwide scale on the prevalence of animal helminthiasis; and control measures are also not satisfactory.

[A Note On Intermediate Hosts Of Paragonimus In Discrict Of Seoul].

Recent results of mass stool examination for parasitic infections revealed 0.01~0.0001 percent of Paragonimus egg positive rate in Seoul school-children.

[Prevalence Of Capillaria Hepatica Among House Rat In Seoul].

During May-July, 1978 a survey on the prevalence of C. hepatica among house rat(Rattus norvegicus Erxl.) and wild mice (Mus musailus molossinus and Microtus mandarinus) in two districts in Seoul was carried out.

[Intestinal Perforation Due To Infection Of Sparganum Mansoni].

In May 1974, authors encountered a 37 year old Korean male who was suffering from very serious condition of acute abdomen. On exploratory laparotomy, a ruptured granulomatous mass in the proximal portion of the ileum showing extensive inflammatory and gangrenous changes was found and about 4 ft. long of the bowel was resected.

[Studies On The Inducing Possibility Of Human Visceral Larva Migrans Associated With Eating Habit Of Raw Liver Of Domestic Animals].

To observe the possibility of human visceral larva migrans due to eating of raw liver of domestic animals, especially of cattle, and also to serve as a good reference for adequate sanitary measures, the investigation survey was carried out from May 1975 to May 1976. From the subjects of a l,048 inhabitants (male 558, female 490) in five localities including two Provinces and three different cities, food habit was studied by questionnaire mannual. Larvae isolated from liver tissues of cattle, and pig were identified.

[Experimental Study On The Effect Of Cortisone In Mice Infected With Toxocara Canis: Histopathological Findings Of Granuloma In The Liver].

The present study was carried out to examine the effect of "Cortisone" on the worm burden migrated in the organs, and granulomatous change in the liver of mice infected with Toxocara canis eggs. Daily does of 0.5 mg "Cortisone" was administrated subcutaneously to Toxocara-infected mice with different schedule.

[Studies on the incidence of the intestinal protopozoa in the mountainous area of Chollapuk-do].

This study was undertaken in order to elucidate the prevalences of the intestinal protozoan infection according to localities and the mode of the infestation in the mountainous area of Chollapuk-do, Korea during the year 1970-1971. 8,508 of fecal samples were collected among the inhabitants of the mountainous area, which covered 18 Myens(=Township) from 6 Guns(=County) in the eastern part of Chollapuk-do. Most of the inhabitants of this area were engaged in farming and living under lower economic and poor sanitary conditions.

[Prevalence Of Intestinal Parasites In Korea].

A survey of intestinal parasites infection among Korean people has been carried out during July 1969 to December 1970. A total of 2,250 stool specimens (male 1,101, female 1,146) was collected from all the provinces and Seoul city in Korea. The specimens were examined routinely by direct fecal smear, zinc sulfate flotation and formalin-ether sedimentation techniques.