A novel soft sensor approach for estimating individual biomass in mixed cultures.

Due to many advantages associated with mixed cultures, their application in biotechnology has expanded rapidly in recent years. At the same time, many challenges remain for effective mixed culture applications. One obstacle is how to efficiently and accurately monitor the individual cell populations.

An improved dynamic method to measure kL a in bioreactors.

An accurate measurement or estimation of the volumetric mass transfer coefficient kL a is crucial for the design, operation, and scale up of bioreactors. Among different physical and chemical methods, the classical dynamic method is the most widely applied method to simultaneously estimate both kL a and cell's oxygen utilization rate. Despite several important follow-up articles to improve the original dynamic method, some limitations exist that make the classical dynamic method less effective under certain conditions.

Impact of pseudo-continuous fermentation on the ethanol tolerance of Scheffersomyces stipitis.

In this work we conducted the pseudo-continuous fermentation, i.e., continuous fermentation with cell retention, using Scheffersomyces stipitis, and studied its effect on ethanol tolerance of the strain.