Development of a duplex droplet digital PCR assay for the detection of complex and in bloodstream infections.

complex (BCC) and are nosocomial pathogens that cause various infections and exhibit high resistance to multiple antimicrobial agents. In this study, we aimed to develop a duplex droplet digital PCR (ddPCR) assay for detecting BCC and in bloodstream infections. We optimized the experimental conditions by setting the annealing temperature to 51°C and determining the optimal concentrations of primers and probes, as well as the thermal cycle numbers.

, gen. nov., sp. nov., a novel bacterial species of the family that could produce flexirubin type pigments.

Two Gram-stain-negative, non-spore-forming, rod-shaped, and obligately aerobic bacteria, designated strains CX-624 and cx-311, were isolated from soil samples in Qinghai Province, China. The two strains grew best at 28 °C on the plate with Tryptone soya agar (TSA). Cells formed circular, convex, translucent, smooth, and orange colonies with approximately 1.

Species-level microbiota of ticks and fleas from in the Qinghai-Tibet Plateau.

Introduction: Ticks and fleas, as blood-sucking arthropods, carry and transmit various zoonotic diseases. In the natural plague foci of China, monitoring of has been continuously conducted in and other host animals, whereas other pathogens carried by vectors are rarely concerned in the Qinghai-Tibet Plateau.

Methods: In this study, we investigated the microbiota of ticks and fleas sampling from in the Plateau, China by metataxonomics combined with metagenomic methods.

Concordance between Genotypic and Phenotypic Drug-Resistant Profiles of Isolates from Taiyuan City, Shanxi Province, China, 2005 to 2016.

Antimicrobial resistance in spp. is a global public health concern. In this study, the AMR phenotypic profiles of 10 kinds of antibiotics were compared with the genotypic profiles using genomic analysis of 218 isolates from Taiyuan City, Shanxi Province, China, 2005 to 2016.

Three-year-period nitrogen additions did not alter soil organic carbon content and lability in soil aggregates in a tropical forest.

Soil immobilizes a considerable proportion of carbon (C) as organic matter in terrestrial ecosystems and is thus critical to stabilize the global climate system. Atmospheric nitrogen (N) deposition could influence soil C storage and stabilization, but how N deposition changes soil organic C (SOC) fractions and lability remains elusive. We investigated the effects of 3-year-period N inputs on SOC fractions and lability along three soil depths (0-10, 10-20, and 20-40 cm) in a tropical forest of southern China.

Development and validation of an advanced fragment analysis-based assay for the detection of 22 pathogens in the cerebrospinal fluid of patients with meningitis and encephalitis.

Background: Meningitis and encephalitis (ME) are central nervous system (CNS) infections mainly caused by bacteria, mycobacteria, fungi, viruses, and parasites that result in high morbidity and mortality. The early, accurate diagnosis of pathogens in the cerebrospinal fluid (CSF) and timely medication are associated with better prognosis. Conventional methods, such as culture, microscopic examination, serological detection, CSF routine analysis, and radiological findings, either are time-consuming or lack sensitivity and specificity.

Clinical application of a multiplex genetic pathogen detection system remaps the aetiology of diarrhoeal infections in Shanghai.

Background: Culture-based diagnostic methods cannot achieve rapid and precise diagnoses for the identification of multiple diarrhoeal pathogens (DPs). A high-throughput multiplex genetic detection system (HMGS) was adapted and evaluated for the simultaneous identification and differentiation of infectious DPs and a broad analysis of DP infection aetiology.

Results: DP-HMGS was highly sensitive and specific for DP detection compared with culture-based techniques and was similar to singleplex real-time PCR.

Subcutaneous injection of hydrogen gas is a novel effective treatment for type 2 diabetes.

Aims/introduction: In previous studies, hydrogen gas (H administration has clearly shown effectiveness in inhibiting diabetes. Here, we evaluated whether subcutaneous injection of H shows enhanced efficacy against type 2 diabetes mellitus induced in mice by a high-fat diet and low-dose streptozotocin treatment.

Material And Methods: H was injected subcutaneously at a dose of 1 mL/mouse/week for 4 weeks.

Validation of a High-Throughput Multiplex Genetic Detection System for Helicobacter pylori Identification, Quantification, Virulence, and Resistance Analysis.

Helicobacter pylori (H. pylori) infection is closely related to various gastroduodenal diseases. Virulence factors and bacterial load of H.

Direct detection of Helicobacter pylori in biopsy specimens using a high-throughput multiple genetic detection system.

Aim: We evaluated the direct high-throughput multiple genetic detection system (dHMGS) for Helicobacter pylori in gastric biopsies.

Materials & Methods: One hundred and thirty-three specimens were concurrently analyzed by dHMGS, rapid urease test, culture and sequencing.

Results: dHMGS was highly sensitive and specific for H.

A high-throughput multiplex genetic detection system for Helicobacter pylori identification, virulence and resistance analysis.

Aim: We established a high-throughput multiplex genetic detection system (HMGS) for identification of Helicobacter pylori with concomitant analysis of virulence and drug resistance.

Materials & Methods: Confirmed 132 H. pylori cultures from gastric biopsies were screened by 20-gene site-HMGS, sequencing and E-test.

Multiple Genetic Analysis System-Based Antibiotic Susceptibility Testing in Helicobacter pylori and High Eradication Rate With Phenotypic Resistance-Guided Quadruple Therapy.

Antibiotics resistance in Helicobacter pylori (H. pylori) is the major factor for eradication failure. Molecular tests including fluorescence in situ hybridization, PCR-restriction fragment length polymorphism, and dual priming oligonucleotide-PCR (DPO-PCR) play critical roles in the detection of antibiotic susceptibility; however, limited knowledge is known about application of multiple genetic analysis system (MGAS) in the area of H.