Association between microbial flora and tissue abnormality around dental implants penetrating the skin in reconstructed oral cancer patients.

Purpose: Patients with skin-penetrating implants sometimes report episodic soft tissue reactions. This pilot clinical trial identified the oral microflora of the peri-implant sulcus of cancer patients with jaw reconstruction and compared sites with and without clinical signs of inflammation.

Materials And Methods: Ten patients were selected during routine follow-up of their implant-supported removable prostheses.

Effects of an antimicrobial additive to toothbrushes on residual periodontal pathogens.

Objective: Previous studies have reported the link between residual microbial contamination of toothbrushes and periodontal diseases. The goal of this pilot study was to evaluate the effects of an antimicrobial additive (Microban) to toothbrushes on residual retention of periodontal pathogens.

Methodology: Twenty patients had one side of their mouths brushed with a toothbrush containing the antimicrobial agent (experimental side), and the other side with a toothbrush containing no agent (control).

Rapid Methods and Automation: A Survey of Professional Microbiologists.

Participants of an international workshop on rapid methods and automation were surveyed concerning the numbers of total plate counts and coliform counts performed per year, the numbers and kinds of pathogen detection tests routinely performed, and the type of instruments and diagnostic kits routinely used in their laboratories. The candid opinions on what is needed in the near future and the general perceptions of the field of rapid methods and automation in microbiology and their wish list were also solicited. Responses from 55 professional practicing microbiologists were analyzed.

Evaluation of Bacterial Diagnostics Kits and Systems at an Instructional Workshop.

Ten commercial bacterial diagnostic systems (AMS, API 20E, AUTOBAC IDX, CATHRA, ENTERIC-TEK, ENTEROTUBE II, MICRO-ID, MINITEK 4 h, MINITEK 24 h and SPECTRUM 10) were evaluated by use of 12 coded enteric bacteria ( Arizona hinshawii , Citrobacter freundii , Enterobacter cloacae , Hafnia alvei , Klebsiella pneumoniae , Morganella morganii , Proteus mirabilis , Proteus vulgaris , Salmonella typhimurium , Serratia marcescens , Shigella dysenteriae and Shigella flexneri ) in two separate workshops (July, 1981 and July, 1982) consisting of 40 participants. Results indicated that most commercial systems provided satisfactory diagnosis (89% to 100%) of these organisms compared to conventional methods. The uniqueness of this study lies in the fact that a group of microbiologists from a variety of geographic locations, training and backgrounds were able to use these systems accurately after only a single exposure to many of the techniques in a workshop environment.

New Methods for Microbiological Analysis of Food.

This review article discusses the application and implications of automated or semi-automated instrumentation as well as miniaturized methods which can be used to detect and characterize microorganisms of importance in the food industry. The instrumentation section includes techniques involving turbidometry, radiometry, fluorometry, immunology, and chromatography. Miniaturized methods include various diagnostic kits and procedures.