Publications by authors named "Miles Mayer"
HIV-1 capsid protein (CA) is essential for viral replication and interacts with numerous host factors to facilitate successful infection. Thus, CA is an integral target for the study of virus-host dynamics and therapeutic development. The multifaceted functions of CA stem from the ability of CA to assemble into distinct structural components that come together to form the mature capsid core.
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- The HIV-1 capsid protein (CA) has different structural forms during replication, which have unique surfaces for interactions, but their specific functions are not well understood due to technical challenges in studying CA.
- Researchers developed CA-targeting aptamers through a branched SELEX approach, identifying subsets that bind specifically either to the CA lattice or the CA hexamer.
- The study evaluated these aptamers to understand binding mechanisms and showcased their ability to purify CA from cell lysates, highlighting their potential as valuable tools for investigating CA's diverse structures.
The HIV-1 capsid protein (CA) assumes distinct assembly forms during replication, each presenting unique, solvent-accessible surfaces that facilitate multifaceted functions and host factor interactions. However, contributions of individual CA assemblies remain unclear, as the evaluation of CA in cells presents several technical challenges. To address this need, we sought to identify CA assembly form-specific aptamers.
View Article and Find Full Text PDFWe report the development of an electrochemical aptamer-based sensor for real time detection of tumor necrosis factor-alpha. The focus of this study is to evaluate the effects of the redox label location on the overall sensor performance, including sensor stability, detection limit, reusability, and selectivity. Three aptamer probes, each labeled with methylene blue (MB) at a specific location, were designed and employed in the fabrication of the sensors.
View Article and Find Full Text PDFSolid supported colorimetric sensing arrays have the advantage of portability and ease of use when deployed in the field, such as crime scenes, disaster zones, or in war zones, but many sensor arrays require complex fabrication methods. Here, we report a practical method for the fabrication of 4 × 4 colorimetric sensor arrays, which are printed on nylon membranes, using a commercially available inkjet printer. In order to test the efficacy of the printed arrays, they were exposed to 43 analytes at concentrations ranging from 0.
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- The study investigates the use of RGB image analysis from colorimetric sensor arrays to identify and quantify different chemical analytes, leveraging image analysis software like ImageJ.!
- Nine chemometric methods were evaluated for their effectiveness in classifying various solutions of acetic acid, malonic acid, lysine, and ammonia, with LDA and KNN performing particularly well with over 90% accuracy.!
- This research expands on existing literature by comparing a broader range of chemometric techniques, highlighting that traditional methods such as PCA, LDA, HCA, and KNN are the most commonly used for colorimetric array analysis.